Synthesis and characterization of bifunctional probes for the specific labeling of fusion proteins

Labeling proteins with synthetic probes is important for studying and characterizing protein function. We have recently introduced a general method for the specific in vivo and in vitro labeling of fusion proteins that is based on the reaction of O6-alkylguanine-DNA alkyltransferase (AGT) with O6-benzylguanine derivs. Here we report two complementary routes for the synthesis of O6-benzylguanine derivs., which allow for the labeling of AGT fusion proteins with bifunctional synthetic probes and demonstrate the specific labeling of AGT fusion proteins with these probes. These mols. should become useful tools for various applications in functional proteomics. [on SciFinder (R)]