Exploring the Mechanical Properties of Single Vimentin Intermediate Filaments by Atomic Force Microscopy

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Organelle abundance is a key microscopic readout of organelle formation and, in many cases, function. Quantification of organelle abundance using confocal microscopy requires estimating their area based on the fluorescence intensity of compartment-specific markers. This analysis usually depends on a user-defined intensity threshold to distinguish organelle regions from the surrounding cytoplasm, which introduces potential bias and variability. To address this issue, we present a machine learning–assisted al

Carbon membranes yielding high selectivity as well as high permeance are attractive to advance the membrane-based gas separation. Herein, we report ultrathin carbon membranes (UCMs) which deliver enhanced gas separation performance through oxygen-modulated pyrolysis of poly(4-vinylpyridine) precursor. We show that O 2 in pyrolysis environment, transforms the otherwise uniform carbon network featuring a ~ 3.9 Å characteristic interlayer spacing into disrupted UCMs (d-UCMs). These d-UCMs possess a multimodal

Structural and functional compromise of the cellular membrane is a central mechanism in the pathogenesis of numerous diseases, including neurodegenerative disorders such as Alzheimer’s and Parkinson’s disease. However, existing techniques for assessing membrane integrity often lack the ability to provide dynamic, whole-cell measurements and are limited to localized damage detection or population-level analysis. There is a growing need for methods capable of monitoring membrane integrity over time at the sin

Zeolitic imidazolate framework‐67 (ZIF‐67) is an important class of nanoporous materials for high‐performance membranes for gas separation, offering uniform‐sized gas‐selective pores. However, the fabrication of high‐quality sub‐100‐nm‐thick ZIF‐67 membranes, critical for high‐performance separation, has remained elusive. Herein, we report a facile strategy to fabricate ∼20‐nm‐thick ZIF‐67 membranes. We identify a simple and rapid route to prepare ∼1‐nm‐thick β‐Co(OH)2 nanosheets as a precursor to ZIF‐67. H

Hydrogels are promising materials for wearable electronic devices. However, maintaining the high sensitivity, self-healing, and excellent adhesion of hydrogels at subzero temperatures remains a challenge. The violet phosphorene-polydopamine hybrid (VP-PDA) was introduced into poly (vinyl alcohol)/ethylene glycol/sodium alginate/sodium tetraborate matrix to achieve an anti-freezing hydrogel with high strain sensitivity, extremely high stretchability (3539 %), rapid self-healing capability, and high adhesion

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In order to realize an implantable glucose sensing system designed within this project, the realization of a selective nanoporous membrane is essential. The final goal of this sub-project is establishing a fabrication process of a semi-permeable membrane consisting of nanoporous layer that has a function to allow diffusion of glucose while ensuring retention of larger molecules.

The development of nanodevices demands for patterning methods in the nanoscale. To bring nanodevices to the market, there is a need for fast, low-cost nanopatterning methods. In addition, an increased flexibility is important for the engineering of multimaterial and multifunctional nano/micro-electro-mechanical systems (NEMS/MEMS), such as polymer-based electronic and sensor devices, 3D microfluidic systems, and bio-analytical systems. A series of alternative surface micro/nanopatterning methods based on ME

Dense planar and tubular oxygen separation membranes of La 0.6Ca0.4Fe0.75Co0.25O3-d were investigated as reactors for the partial oxidation (POX) of methane to syngas. Disk-shaped membranes were prepared by compaction and tubes by extrusion. Their permeation properties were measured in an air/argon pO2 gradient as a function of temperature. At 900°C, the oxygen flux through a 1.26 mm thick membrane was 0.075 micromol/cm2s and through a 0.25 mm thick tube, 0.24 micromol/cm2s. For the POX measurements, a cata

In this study, we examine the parameters that govern the overall oxygen flux through a permeation membrane. The chemical diffusion (D) and the surface exchange (k) coefficients for oxygen in La0.6Sr0.4Fe0.8Co0.2O3-d were determined as a function of temperature using a specially designed electrochemical cell combined with impedance spectroscopy. Typically, D=1.7 10-5 cm2/s and k=3.6 10-4 cm/s in air at 900°C. These values were compared with literature 18O/16O isotope exchange data. Oxygen permeation measurem

A novel in situ product removal (ISPR) method that uses microcapsules to ext. inhibitory products from the reaction suspension is introduced into fermn. technol. More specifically, L-phenylalanine (L-phe) was transformed by Saccharomyces cerevisiae to 2-phenylethanol (PEA), which is inhibitory toward the yeast. In order to continuously remove PEA from the vicinity of the cells, the reaction suspension was brought into contact with capsules of 2.2-mm diam. that had a hydrophobic core of di-Bu sebacate and an

The analogous techniques of photoactivation of fluorescence (PAF) and fluorescence recovery after photobleaching (FRAP) have been applied previously to the study of actin dynamics in living cells. Traditionally, separate experiments estimate the mobility of actin monomer or the lifetime of actin filaments. A mathematical description of the dynamics of the actin cytoskeleton, however, predicts that the evolution of fluorescence in PAF and FRAP experiments depends simultaneously on the diffusion coefficient o

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Exploring the Mechanical Properties of Single Vimentin Intermediate Filaments by Atomic Force Microscopy

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Machine Learning-Assisted Quantification of Organelle Abundance

Oxygen-induced multimodal ultramicroporous structure in 10-nm-thick carbon membranes for enhanced hydrogen separation

A microfluidic platform for whole-membrane integrity profiling in live neuronal cells

A microfluidic platform for whole-membrane integrity profiling in live neuronal cells

Facile Synthesis of 20‐nm‐Thick ZIF‐67 Films for Hydrogen Sieving Using β‐Co(OH)2 Precursor Nanosheets

Subzero fast self-healing and adhesive violet phosphorene incorporated sodium alginate-based hydrogel as strain sensors

Towards a kinetic model of the phospholipids biosynthesis of the yeast Saccharomyces cerevisiae

Small strain mechanical properties of latex-based nanocomposite films

Implantable Glucose Sensor, IGLUS III – Membrane Technology –

Emerging nanopatterning methods based on MEMS tools

Planar and Tubular perovskite-type membrane reactors for the partial oxidation of methane to syngas

Oxygen transport through dense La0.6Sr0.4Fe0.8Co0.2O3-d perovskite-type permeation membranes

xxvel type of in situ extraction: Use of solvent containing microcapsules for the bioconversion of 2-phenylethanol from L-phenylalanine by Saccharomyces cerevisiae

Simultaneous measurements of actin filament turnover, filament fraction, and monomer diffusion in endothelial cells

Viscoelastic properties of soft tissues : application to knee ligaments and tendons