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The sustainable development of rivers requires a knowledge on the three-dimensional mean flow field and the turbulence in complex morphologies. In a future, the computational capacity will be sufficient to simulate numerically the fine details of the flow. Our physical knowledge, however, is at present insufficient: the overwhelming majority of experimental research concerns straight-uniform flow and even complex numerical models are based on straight-uniform-flow knowledge. A sound understanding of the relevant physical processes will always be essential in complicated problems such as the river management, which concern a variety of different fields, and this irrespective of the available computational capacity. This PhD investigates, mainly experimentally, the mean-flow field and the turbulence in open-channel bends; this situation is considered as a generic case for complex highly three-dimensional flow. The experimental investigation is rendered feasible by the availability of a powerful Acoustic Doppler Velocity Profiler (ADVP), developed in our laboratory. The principal objectives of this PhD are: To provide a high-quality data base on three-dimensional open-channel flow, including all three mean velocity components and all six Reynolds stresses on a fine grid. To document interesting features of the flow field and the turbulence, such as the multi-cellular pattern of secondary circulation, the curvature influence on the turbulence, etc. To gain insight in the relevant physical mechanisms and processes underlying these features. To apply the acquired knowledge in an engineering sense, mainly by evaluating, improving and developing numerical simulation techniques. First, a limited series of experiments was conducted in a small laboratory flume, with the aim of testing the feasibility of the project. Subsequently, extended series of experiments have been designed in a large and optimized laboratory flume. The small-flume experiments yielded results beyond all expectations and form the core of this dissertation. The large-flume experiment are intended to confirm those results and to investigate newly emerged questions. Only few large-flume results are included in this dissertation; more large-flume results will be reported in literature in the future. The structure of this dissertation follows the above-mentioned objectives. In PART I "Instrumentation and experimental set-up", the experimental set-up, the ADVP and the measuring strategy are presented. Furthermore, a method is proposed to improve acoustic turbulence measurements. PART II "Experimental observations" provides high-quality data on the mean flow and the turbulence and documents the most interesting features: The downstream velocity increases in outward direction and its vertical profiles are flatter (increased/decreased velocities in the lower/upper part of the flow depth) than in straight flow. A relatively small and weak outer-bank cell of secondary circulation exists besides