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Self-assembled melamine microlens arrays for immunofluorescence enhancement

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Enhanced Room-Temperature Photoluminescence Quantum Yield in Morphology Controlled J-Aggregates

Frank Nüesch, Jakob Heier, Surendra Babu Anantharaman

Supramolecular assemblies from organic dyes forming J-aggregates are known to exhibit narrowband photoluminescence with full-width at half maximum of approximate to 9 nm (260 cm(-1)). Applications of these high color purity emitters, however, are hampered ...

2021

Self-Assembly of Microscopic Rods Due to Depletion Interaction

Ignacio Pagonabarraga Mora

In this article, using numerical simulations we investigate the self-assembly of rod-like particles in suspension due to depletion forces which naturally emerge due to the presence of smaller spherical depletant particles. We characterize the type of clust ...

2020

Monitoring alpha-synuclein oligomerization and aggregation using bimolecular fluorescence complementation assays: What you see is not always what you get

Hilal Lashuel, Nathan Alain Denis Riguet

Bimolecular fluorescence complementation (BiFC) was introduced a decade ago as a method to monitor alpha-synuclein (alpha-syn) oligomerization in intact cells. Since then, several alpha-syn BiFC cellular assays and animal models have been developed based o ...

Wiley-Blackwell2021

Chemistry and Analysis of Nanoparticle-Modified Living Cells

Tanja Thomsen

Conventional therapeutics are often limited by their targeting ability, resulting in harmful and potential fatal side-effects for the patients. Recently, new strategies have been developed to improve target specificity of drugs in order to generate more ef ...

EPFL2020

A general strategy to develop cell permeable and fluorogenic probes for multicolour nanoscopy

Kai Johnsson, Lu Wang, Lin Xue

It is difficult to develop suitable fluorescent probes for live-cell nanoscopy, but a general strategy is now reported that can transform regular fluorophores into fluorogenic probes with excellent cell permeability and low unspecific background signals. U ...

NATURE PUBLISHING GROUP2020

Active illumination and computational Methods for Temporal and Spectral Super-Resolution Microscopy

Christian Jaques

Light microscopy is a tool of paramount importance for biologists and has been constantly improved for the past four centuries. Despite many recent developments, microscopy techniques still require improvement, especially to reach better temporal and spect ...

EPFL2020

Temporal resolution doubling in fluorescence light-sheet microscopy via a hue-encoded shutter and regularization

Michael Stefan Daniel Liebling, Christian Jaques

Studying dynamic biological processes, such as heart development and function in zebrafish embryos, often relies on multi-channel fluorescence labeling to distinguish multiple anatomical features, yet also demands high frame rates to capture rapid cell mot ...

2020

Fluorogenic probes for live-cell imaging

Aleksandar Salim

The development of novel fluorogenic probes and their use in live-cell imaging lead to a plethora of discoveries in biological research. Herein I report on novel red fluorogenic probes for live-cell super-resolution microscopy of various cellular organelle ...

EPFL2020

Finite element modeling of dynamic frictional rupture with rate and state friction

Jean-François Molinari, Fabian Barras, Roozbeh Rezakhani

Numerous laboratory experiments have demonstrated the dependence of the friction coefficient on the interfacial slip rate and the contact history, a behavior generically called rate and state friction. Although numerical models have been widely used for an ...

PERGAMON-ELSEVIER SCIENCE LTD2020

Photoactivatable Silicon Rhodamines for Super-Resolution Microscopy

Michelle Susanne Frei

Fluorescence microscopy is the method of choice to monitor dynamic processes in living cells due to its non-invasive nature. A variety of different fluorophores and labeling systems are currently used to selectively visualise structures or biomolecules of ...

EPFL2020

Fluorescence lifetime imaging with a megapixel SPAD camera and neural network lifetime estimation

Edoardo Charbon, Claudio Bruschini, Ming-Lo Wu, Kazuhiro Morimoto

Fluorescence lifetime imaging microscopy (FLIM) is a key technology that provides direct insight into cell metabolism, cell dynamics and protein activity. However, determining the lifetimes of different fluorescent proteins requires the detection of a rela ...

NATURE RESEARCH2020

Routes for Efficiency Enhancement in Fluorescent TADF Exciplex Host OLEDs Gained from an Electro-Optical Device Model

Frank Nüesch, Markus Regnat

Fluorescence-based organic light-emitting diodes (OLEDs) using thermally activated delayed fluorescence (TADF) have increasingly attracted attention in research and industry. One method to implement TADF is based on an emitter layer composed of an exciplex ...

WILEY2020

Single-Photon, Time-Gated, Phasor-Based Fluorescence Lifetime Imaging through Highly Scattering Medium

Edoardo Charbon, Claudio Bruschini, Arin Can Ülkü

Fluorescence lifetime imaging (FLI) is increasingly recognized as a powerful tool for biochemical and cellular investigations, including in vivo applications. Fluorescence lifetime is an intrinsic characteristic of any fluorescent dye which, to a large ext ...

AMER CHEMICAL SOC2020

Single-molecule studies of amyloid proteins: from biophysical properties to diagnostic perspectives

Chan Cao

In neurodegenerative diseases, a wide range of amyloid proteins or peptides such as amyloid-beta and alpha-synuclein fail to keep native functional conformations, followed by misfolding and self-assembling into a diverse array of aggregates. The aggregates ...

CAMBRIDGE UNIV PRESS2020

Propagation of fluid driven fractures in transversely isotropic material

Fatima-Ezzahra Moukhtari

The propagation of fluid driven fractures is used in a number of industrial applications (well stimulation of unconventional reservoirs, development of deep geothermal systems) but also occurs naturally (magmatic dyke intrusion). While the mechanics of hyd ...

EPFL2020