Publications by Simone Allazetta

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Stem cells play a key role in a wide range of biological processes, in large part due to their ability to self-renew or differentiate into specialized cell types in response to various biological cues. In vivo, stem cells reside in a complex microenvironme ...

Microfluidic gradient systems offer a very precise means to probe the response of cells to graded biomolecular signals in vitro, for example to model how morphogen proteins affect cell fate during developmental processes. However, existing gradient makers ...

Stem cells reside in complex niches in which their behaviour is tightly regulated by various biochemical and biophysical signals. In order to unveil some of the crucial stem cell-niche interactions and expedite the implementation of stem cells in clinical ...

Computer-controlled hydrodynamic flow focusing was utilized to generate tethered protein gradients of any user-defined shape on the surface of soft synthetic hydrogels. ...

As the field of tissue engineering develops, methods for screening combinations of signals for their effects on stem cell behavior are needed. We introduce a microgel-based screening platform for testing combinations of in situ-generated proteins on stem c ...

A microfluidic in vitro cell encapsulation platform to systematically test the effects of microenvironmental parameters on cell fate in 3D is developed. Multiple cell types including fibroblasts, embryonic stem cells, and cancer cells are incorporated in e ...

The invention relates to a scaffold material comprising a carrier and embedded microbeads for use in tissue engineering applications such as soft tissues therapeutic treatment. The scaffold provides a short-term bulking effect coupled with a long-term func ...

Simone Allazetta

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Microfluidic engineering of artificial stem cell niches

Patterning of cell-instructive hydrogels by hydrodynamic flow focusing

Stem cell niche engineering through droplet microfluidics

Programmable microfluidic patterning of protein gradients on hydrogels

High-throughput stem cell-based phenotypic screening through microniches

Cell-Instructive Microgels with Tailor-Made Physicochemical Properties

Natural polymer-derived scaffold material and methods for production thereof