Lara Buscemi Estefanell

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Tracking hyperpolarized (HP) ¹³C labeling from either [1-¹³C] pyruvate or [1-¹³C] lactate is a useful tool to assess intermediary metabolism in vivo, which has already been translated from preclinical to clinical research. HP [1-¹³C] pyruvate and [1-¹³C] l ...

Myofibroblasts promote tissue contractures during fibrotic diseases. To understand how spontaneous changes in the intracellular calcium concentration, Ca2+, contribute to myofibroblast contraction, we analysed both Ca2+ and subcellular contractio ...

We propose a new technique to measure the volume of adherent migrating cells. The method is based on a negative staining where a fluorescent, non-cell-permeant dye is added to the extracellular medium. The specimen is observed with a conventional fluoresce ...

Repeated passaging in conventional cell culture reduces pluripotency and proliferation capacity of human mesenchymal stem cells (MSC). We introduce an innovative cell culture method whereby the culture surface is dynamically enlarged during cell proliferat ...

Background: TGF-beta 1 controls many pathophysiological processes including tissue homeostasis, fibrosis, and cancer progression. Together with its latency-associated peptide (LAP), TGF-beta 1 binds to the latent TGF-beta 1-binding protein-1 (LTBP-1), whic ...

Strain devices with expandable polydimethylsiloxane (PDMS) culture membranes are frequently used to stretch cells in vitro, mimicking mechanically dynamic tissue environments. To immobilize cell-adhesive molecules to the otherwise non-adhesive PDMS substra ...

Changes in the mechanical properties of dermis occur during skin aging or tissue remodeling and affect the activity of resident fibroblasts. With the aim to establish elastic culture substrates that reproduce the variable softness of dermis, we determined ...

Hyperpolarization of 13C by dissolution dynamic nuclear polarization (dDNP) boosts the sensitivity of magnetic resonance spectroscopy (MRS), making possible the monitoring in vivo and in real time of the biochemical reactions of exogenously infused 13C-lab ...

Cells with irregular shapes, numerous long thin filaments, and morphological similarities to the gastrointestinal interstitial cells of Cajal (ICCs) have been observed in the wall of some blood vessels. These ICC-like cells (ICC-LCs) do not correspond to t ...

Lara Buscemi Estefanell

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Tracking Hyperpolarized [1-¹³C] Pyruvate and [1-¹³C] L-Lactate Metabolism in the Healthy and Post-Stroke Mouse Brain

A New Lock-step Mechanism of Matrix Remodelling Based on Subcellular Contractile Events

Dynamic measurement of the height and volume of migrating cells by a novel fluorescence microscopy technique

A novel method of dynamic culture surface expansion improves mesenchymal stem cell proliferation and phenotype

The Single-Molecule Mechanics of the Latent TGF-beta 1 Complex

The covalent attachment of adhesion molecules to silicone membranes for cell stretching applications

The Nano-Scale Mechanical Properties of the Extracellular Matrix Regulate Dermal Fibroblast Function

Influence of DNP Polarizing Agents on Biochemical Processes: TEMPOL in Transient Ischemic Stroke

Identification and functional response of interstitial Cajal-like cells from rat mesenteric artery