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Birgit Mollwitz

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Related publications (5)

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Structural Studies and Protein Engineering of Human O6-Alkylguanine-DNA Alkyltransferase

Birgit Mollwitz

The specific labeling of proteins with synthetic probes is a powerful approach to study protein function and protein tags have been widely used for this purpose. A well-established example for a self-labeling protein tag is SNAP-tag. It specifically reacts ...

EPFL2012

Visualizing Biochemical Activities in Living Cells through Chemistry

Kai Johnsson, Luc Reymond, Rudolf Griss, Alberto Schena, Grazvydas Lukinavicius, Matthias Albert Brun, Karolina Bojkowska, Birgit Mollwitz, Anastasiya Masharina

The development of molecular probes to visualize cellular processes is an important challenge in chemical biology. One possibility to create such cellular indicators is based on the selective labeling of proteins with synthetic probes in living cells. Over ...

2011

Mechanism to trigger unfolding in alkylguanine alkyltransferase

Ursula Röthlisberger, Elizabeth Claire Brunk, Birgit Mollwitz

Amer Chemical Soc2013

Directed evolution of the suicide protein O⁶-alkylguanine-DNA alkyltransferase for increased reactivity results in an alkylated protein with exceptional stability

Ursula Röthlisberger, Kai Johnsson, Florence Pojer, Elizabeth Claire Brunk, Birgit Mollwitz, Simone Schmitt

Here we present a biophysical, structural, and computational analysis of the directed evolution of the human DNA repair protein O-6-alkylguanine-DNA alkyltransferase (hAGT) into SNAP-tag, a self-labeling protein tag. Evolution of hAGT led not only to incre ...

2012

Mechanism to Trigger Unfolding in O6--Alkylguanine-DNA Alkyltransferase

Ursula Röthlisberger, Elizabeth Claire Brunk, Birgit Mollwitz

O6-alkylguanine-DNA alkyltransferase (AGT) adopts a non-enzymatic suicide mechanism for the repair of methylated guanine bases by transferring the methyl adduct to itself, thereby initiating unfolding and fast degradation. Classical molecular dynamics simu ...

Wiley-Blackwell2013