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Johanna Generosi

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Related publications (5)

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Biological samples studied by optical nanospectroscopy

Johanna Generosi

The different parts of the electromagnetic spectrum result in diverse effects upon interaction with matter: according to the wavelength, the radiation has energy appropriate for the excitation of a specific physical process. X-rays can be used as a tool to ...

EPFL2009

Infrared scanning near-field optical microscopy investigates order and clusters in model membranes

Giorgio Margaritondo, Johanna Generosi

Due to its surface sensitivity and high spatial resolution, scanning near-field optical microscopy (SNOM) has a significant potential to study the lateral organization of membrane domains and clusters. Compared to other techniques, infrared near-field micr ...

2008

AMPA receptor imaging by infrared scanning near-field optical microscopy

Giorgio Margaritondo, Kai Johnsson, Harald Hirling, Johanna Generosi

Infrared scanning near-field optical microscopy is used to detect AMPA-type glutamate receptor clusters in hippocampal neuron cells. AMPA's GluR2-subunits were marked with the fluorophore Alexa488 and high-resolution micrographs were taken at 6.25 µm wavel ...

Wiley2008

Photobleaching-free infrared near-field microscopy localizes molecules in neurons

Giorgio Margaritondo, Kai Johnsson, Harald Hirling, Johanna Generosi

High-resolution detection of specific molecules in cells is a major challenge in biology. We show that infrared scanning near-field microscopy can detect the spatial distribution of alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid–type glutamate r ...

2008

Spectroscopic infrared near-field microscopy and x-ray reflectivity studies of order and clustering in lipid membranes

Giorgio Margaritondo, Johanna Generosi

Lipid membranes were studied by infrared scanning near-field optical microscopy at several wavelengths and by x-ray reflectivity. Together with the x-ray data, the optical images indicate the formation of locally ordered multiple bilayers, and the topograp ...

AIP American Institute of Physics2006