Publications by Romain Zufferey

Related publications (14)

Organ distribution of gene expression after intravenous infusion of targeted and untargeted lentiviral vectors

Lentiviral vectors represent an attractive technology platform from which to develop a targetable injectable gene delivery system for transduction of specific cell populations in vivo, irrespective of their cell cycle status. Targeted HIV-1-based lentivira ...

2001

Self-inactivating lentiviral vectors with enhanced transgene expression as potential gene transfer system in Parkinson's disease

Patrick Aebischer, Didier Trono, Jean-Charles Bensadoun, Romain Zufferey

Glial cell line-derived neurotrophic factor (GDNF) is able to protect dopaminergic neurons against various insults and constitutes therefore a promising candidate for the treatment of Parkinson's disease. Lentiviral vectors that infect quiescent neuronal c ...

2000

A third-generation lentivirus vector with a conditional packaging system

Didier Trono, Romain Zufferey

Vectors derived from human immunodeficiency virus (HIV) are highly efficient vehicles for in vivo gene delivery. However, their biosafety is of major concern. Here we exploit the complexity of the HIV genome to provide lentivirus vectors with novel biosafe ...

American Society for Microbiology1998

Woodchuck hepatitis virus posttranscriptional regulatory element enhances expression of transgenes delivered by retroviral vectors

Didier Trono, Romain Zufferey

The expression of genes delivered by retroviral vectors is often inefficient, a potential obstacle for their widespread use in human gene therapy. Here, we explored the possibility that the posttranscriptional regulatory element of woodchuck hepatitis viru ...

American Society for Microbiology1999

Induction of A9 dopaminergic neurons from neural stem cells improves motor function in an animal model of Parkinsons disease

Romain Zufferey

Neural stem cells (NSCs) are widely endorsed as a cell source for replacement strategies in neurodegenerative disease. However, their usefulness is currently limited by the inability to induce specific neurotransmitter phenotypes in these cells. In order t ...

Oxford University Press2008

Self-inactivating lentivirus vector for safe and efficient in vivo gene delivery

Didier Trono, Romain Zufferey

In vivo transduction of nondividing cells by human immunodeficiency virus type 1 (HIV-1)-based vectors results in transgene expression that is stable over several months. However, the use of HIV-1 vectors raises concerns about their safety. Here we describ ...

American Society for Microbiology1998

Lentivirus vector gene expression during ES cell-derived hematopoietic development in vitro

Didier Trono, Romain Zufferey

The murine embryonal stem (ES) cell virus (MESV) can express transgenes from the long terminal repeat (LTR) promoter/enhancer in undifferentiated ES cells, but expression is turned off upon differentiation to embryoid bodies (EBs) and hematopoietic cells i ...

American Society for Microbiology2000

Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo

Didier Trono, Romain Zufferey

Retroviral vectors derived from lentiviruses such as HIV-1 are promising tools for human gene therapy because they mediate the in vivo delivery and long-term expression of transgenes in nondividing tissues. We describe an HIV vector system in which the vir ...

1997

A stable system for the high-titer production of multiply attenuated lentiviral vectors

Didier Trono, Romain Zufferey

Lentiviral vectors open exciting perspectives for the genetic treatment of a wide array of inherited and acquired diseases, owing to their ability to govern the efficient delivery, integration, and long-term expression of transgenes into nondividing cells ...

2000

Lentivirus-mediated Bcl-2 expression in betaTC-tet cells improves resistance to hypoxia and cytokine-induced apoptosis while preserving in vitro and in vivo control of insulin secretion

Didier Trono, Romain Zufferey

betaTC-tet cells are conditionally immortalized pancreatic beta cells which can confer long-term correction of hyperglycemia when transplanted in syngeneic streptozocin diabetic mice. The use of these cells for control of type I diabetes in humans will req ...

1999

Lentiviral vector-mediated genetic modification of human neural progenitor cells for ex vivo gene therapy

Patrick Aebischer, Bernard Schneider, Romain Zufferey

Human neural progenitor cells (hNPC) hold great potential as an ex vivo system for delivery of therapeutic proteins to the central nervous system. When cultured as aggregates, termed neurospheres, hNPC are capable of significant in vitro expansion. In the ...

2007

Response of aged parkinsonian monkeys to in vivo gene transfer of GDNF

Patrick Aebischer, Romain Zufferey

This study assessed the potential for functional and anatomical recovery of the diseased aged primate nigrostriatal system, in response to trophic factor gene transfer. Aged rhesus monkeys received a single intracarotid infusion of MPTP, followed one week ...

2009

Salivary glands and gene therapy: the mouth waters

Patrick Aebischer, Romain Zufferey

2004

Lentiviral gene transfer into primary and secondary NOD/SCID repopulating cells

Didier Trono, Romain Zufferey

The ability of lentiviral vectors to transfer genes into human hematopoietic stem cells was studied, using a human immunodeficiency virus 1 (HIV-1)-derived vector expressing the green fluorescence protein (GFP) downstream of the phosphoglycerate kinase (PG ...

American Society of Hematology2000