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Graham Knott

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Related publications (97)

Page 1 of 5

Nano-imaging trace elements at organelle levels in substantia nigra overexpressing alpha-synuclein to model Parkinson's disease

Philippe André Guy Colin, Bernard Schneider, Graham Knott

Sub-cellular trace element quantifications of nano-heterogeneities in brain tissues offer unprecedented ways to explore at elemental level the interplay between cellular compartments in neurodegenerative pathologies. We designed a quasi-correlative method ...

NATURE PUBLISHING GROUP2020

Nigrostriatal overabundance of alpha-synuclein leads to decreased vesicle density and deficits in dopamine release that correlate with reduced motor activity

Patrick Aebischer, Marco Cantoni, Jean-Charles Bensadoun, Graham Knott, Ralf Schneggenburger, Özgür Genç, Wojciech Janusz Bobela

alpha-Synuclein (alpha-syn) is a presynaptic protein present at most nerve terminals, but its function remains largely unknown. The familial forms of Parkinson's disease associated with multiplications of the alpha-syn gene locus indicate that overabundanc ...

Springer-Verlag2012

Impairment of Glycolysis-Derived L-Serine Production in Astrocytes Contributes to Cognitive Deficits in Alzheimer's Disease

Graham Knott

Alteration of brain aerobic glycolysis is often observed early in the course of Alzheimer's disease (AD). Whether and how such metabolic dysregulation contributes to both synaptic plasticity and behavioral deficits in AD is not known. Here, we show that th ...

Cell Press2020

Diversity of Cortico-descending Projections: Histological and Diffusion MRI Characterization in the Monkey

Jean-Philippe Thiran, Graham Knott, Giorgio Innocenti, Maxime Descoteaux

The axonal composition of cortical projections originating in premotor, supplementary motor (SMA), primary motor (a4), somatosensory and parietal areas and descending towards the brain stem and spinal cord was characterized in the monkey with histological ...

OXFORD UNIV PRESS INC2019

Combined deletion of Glut1 and Glut3 impairs lung adenocarcinoma growth

Bernard Lanz, Graham Knott, Rolf Gruetter, Stéphanie Clerc, Nadine Fournier, Caroline Anne-Lyse Contat, Mario Gaetano Lepore, Etienne Meylan, Anders Meibom, Stéphane Laurent Escrig, Justine Pascual, Louise Helene Soegaard Jensen, Pierre-Benoit Bernard Ancey, Silvia Sabatino

Glucose utilization increases in tumors, a metabolic process that is observed clinically by F-18-fluorodeoxyglucose positron emission tomography (F-18-FDG-PET). However, is increased glucose uptake important for tumor cells, and which transporters are impl ...

2020

Mechanisms of axoneme and centriole elimination in Naegleria gruberi

Graham Knott, Pierre Gönczy, Mary-Claude Croisier-Coeytaux, Coralie Busso, Friso Edsger Douma, Gabriela Garcia Rodriguez, Alexander Woglar

The early branching eukaryote Naegleria gruberi can transform transiently from an amoeboid life form lacking centrioles and flagella to a flagellate life form where these elements are present, followed by reversion to the amoeboid state. The mechanisms imp ...

SPRINGERNATURE2024

Mitofusin-2 in nucleus accumbens regulates anxiety and depression-like behaviors through mitochondrial and neuronal actions

Graham Knott, Maria del Carmen Sandi Perez

Background Emerging evidence points at a central role of mitochondria in psychiatric disorders. However, little is known about the molecular players that regulate mitochondria in neural circuits regulating anxiety and depression, and how they impact on neu ...

2021

GABA Signaling Promotes Synapse Elimination and Axon Pruning in Developing Cortical Inhibitory Interneurons

Graham Knott

Accumulating evidence indicates that GABA acts beyond inhibitory synaptic transmission and regulates the development of inhibitory synapses in the vertebrate brain, but the underlying cellular mechanism is not well understood. We have combined live imaging ...

2012

PGC-1 alpha activity in nigral dopamine neurons determines vulnerability to alpha-synuclein

Bernard Schneider, Graham Knott, Wojciech Janusz Bobela, Lu Zheng

Introduction: Mitochondrial dysfunction and oxidative stress are critical factors in the pathogenesis of age-dependent neurodegenerative diseases. PGC-1 alpha, a master regulator of mitochondrial biogenesis and cellular antioxidant defense, has emerged as ...

2015

Analysis of centriole elimination during C. elegans oogenesis

Graham Knott, Pierre Gönczy, Tamara Mikeladze-Dvali, Petr Strnad, Lukas von Tobel

Centrosomes are the principal microtubule organizing centers (MTOCs) of animal cells and comprise a pair of centrioles surrounded by pericentriolar material (PCM). Centriole number must be carefully regulated, notably to ensure bipolar spindle formation an ...

2012

Automated Detection and Segmentation of Synaptic Contacts in Nearly Isotropic Serial Electron Microscopy Images

Marco Cantoni, Graham Knott

We describe a protocol for fully automated detection and segmentation of asymmetric, presumed excitatory, synapses in serial electron microscopy images of the adult mammalian cerebral cortex, taken with the focused ion beam, scanning electron microscope (F ...

2011

ADAMTS18(+) villus tip telocytes maintain a polarized VEGFA signaling domain and fenestrations in nutrient-absorbing intestinal blood vessels

Graham Knott

The small intestinal villus tip is the first point of contact for lumen-derived substances including nutrients and microbial products. Electron microscopy studies from the early 1970s uncovered unusual spatial organization of small intestinal villus tip bl ...

Nature Portfolio2022

Rapid Functional Maturation of Nascent Dendritic Spines

Graham Knott

Spine growth and retraction with synapse formation and elimination plays an important role in shaping brain circuits during development and in the adult brain, yet the temporal relationship between spine morphogenesis and the formation of functional synaps ...

2009

FOXO3 determines the accumulation of alpha-synuclein and controls the fate of dopaminergic neurons in the substantia nigra

Juan-Carlos Sarria, Bernard Schneider, Graham Knott, Emilda Pino, Lu Zheng

Parkinson's disease (PD) is characterized by the selective degeneration of neuronal populations presumably due to pathogenic interactions between aging and predisposing factors such as increased levels of a-synuclein. Here, we genetically modulate the acti ...

Oxford University Press2014

A protocol for preparing GFP-labeled neurons previously imaged in vivo and in slice preparations for light and electron microscopic analysis

Graham Knott

In vivo imaging of green fluorescent protein (GFP)-labeled neurons in the intact brain is being used increasingly to study neuronal plasticity. However, interpreting the observed changes as modifications in neuronal connectivity needs information about syn ...

Nature Publishing Group2009

Pansynaptic Enlargement at Adult Cortical Connections Strengthened by Experience

Graham Knott

Behavioral experience alters the strength of neuronal connections in adult neocortex. These changes in synaptic strength are thought to be central to experience-dependent plasticity, learning, and memory. However, it is not known how changes in synaptic tr ...

Oxford University Press2014

Synaptic proximity enables NMDAR signalling to promote brain metastasis

Graham Knott, Douglas Hanahan, Mohammad Sadegh Saghafinia, Iacovos Michael, Qiqun Zeng, Brian Donal McCabe, Wei Jiao

Metastasis-the disseminated growth of tumours in distant organs-underlies cancer mortality. Breast-to-brain metastasis (B2BM) is a common and disruptive form of cancer and is prevalent in the aggressive basal-like subtype, but is also found at varying freq ...

2019

NeuroMorph: A Software Toolset for 3D Analysis of Neurite Morphology and Connectivity

Graham Knott, Jérôme Blanc, Anne Alison Jorstad

The geometries of axons, dendrites and their synaptic connections provide important information about their functional properties. These can be collected directly from measurements made on serial electron microscopy images. However, manual and automated se ...

2018

Dysfunction of homeostatic control of dopamine by astrocytes in the developing prefrontal cortex leads to cognitive impairments

Nicole Déglon, Graham Knott, Corrado Cali

Astrocytes orchestrate neural development by powerfully coordinating synapse formation and function and, as such, may be critically involved in the pathogenesis of neurodevelopmental abnormalities and cognitive deficits commonly observed in psychiatric dis ...

2020

Stable isotope labeling and ultra-high-resolution NanoSIMS imaging reveal alpha-synuclein-induced changes in neuronal metabolism in vivo

Bernard Schneider, Graham Knott, Bohumil Maco, Anders Meibom, Stéphane Laurent Escrig, Sofia Carmen Suzanne Spataro, Louise Helene Soegaard Jensen

In Parkinson's disease, pathogenic factors such as the intraneuronal accumulation of the protein alpha-synuclein affect key metabolic processes. New approaches are required to understand how metabolic dysregulations cause degeneration of vulnerable subtype ...

2023