Loïc Dayon

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Related publications (31)

Proteomics of Cerebrospinal Fluid: Throughput and Robustness Using a Scalable Automated Analysis Pipeline for Biomarker Discovery

Cerebrospinal fluid (CSF) is a body fluid of high clinical relevance and an important source of potential biomarkers for brain-associated damages, such as traumatic brain injury and stroke, and for brain diseases, such as Alzheimer's and Parkinson's. Herei ...

Amer Chemical Soc2015

Proteomics and circadian rhythms: It's all about signaling!

Loïc Dayon, Martin Kussmann, Daniel Alain Dominique Marie Mauvoisin, Frédéric Bruno Martin Gachon

Proteomic technologies using MS offer new perspectives in circadian biology, in particular the possibility to study PTMs. To date, only very few studies have been carried out to decipher the rhythmicity of protein expression in mammals with large-scale pro ...

Wiley-Blackwell2015

Comprehensive and Scalable Highly Automated MS-Based Proteomic Workflow for Clinical Biomarker Discovery in Human Plasma

Loïc Dayon, Martin Kussmann

Over the past decade, mass spectrometric performance has greatly improved in terms of sensitivity, dynamic range, and speed. By contrast, only limited progress has been accomplished with regard to automation, throughput, and robustness of the proteomic sam ...

Amer Chemical Soc2014

Combination of Gas-Phase Fractionation and MS3 Acquisition Modes for Relative Protein Quantification with Isobaric Tagging

Loïc Dayon, Martin Kussmann

Relative quantification of peptides and proteins with isobaric tags such as iTRAQ or TMT is commonly used in comparative quantitative proteomics based on tandem mass spectrometry (MS/MS). Nonetheless, isobaric tagging inherently suffers from the cofragment ...

Amer Chemical Soc2012

Electrospray Micromixer Chip for On-Line Derivatization and Kinetic Studies

Hubert Girault, Niels Erik Lion, Loïc Dayon, Brice Perruche, Mélanie Abonnenc

An electrospray microchip for mass spectrometry comprising an integrated passive mixer to carry out on-chip chemical derivatizations is described. The microchip fabricated using UV-photoablation is composed of two microchannels linked together by a liquid ...

2008

Diagonal chromatographic selection of cysteinyl peptides modified with benzoquinones

Hubert Girault, Loïc Dayon

The derivatization of cysteine-containing peptides with benzoquinone compounds is rapid, quantitative and specific in acidic media. The conversion of cysteines into hydrophobic benzoquinone-adducted residues in peptides is used here to alter the chromatogr ...

Springer Verlag2007

Multitrack electrospray chips

Hubert Girault, Michel Prudent, Niels Erik Lion, Loïc Dayon, Mélanie Abonnenc

Multitrack electrospray chips (MTEC) were fabricated by UV-photoablation of polyethylene terephthalate (PET) substrates. They are composed of an array of up to six microchannels that are successively used as electrospray ionization (ESI) emitters for mass ...

Wiley-Blackwell2006

Thiol-targeted microspray mass spectrometry of peptides and proteins through on-line EC-tagging

Loïc Dayon

Modification strategies targeting specific amino acids in proteins are widespread in proteomic analysis. Cysteine residues have received deep consideration in view of their nucleophilic properties and their occurrence in the proteome. A recently developed ...

EPFL2006

Probing Cysteine Reactivity in Proteins by Mass Spectrometric EC-Tagging

Hubert Girault, Christophe Roussel, Loïc Dayon

The on-line electrochemical tagging (EC-tagging) of cysteine residues in proteins during mass spectrometry is studied to probe the cysteine environment. Benzoquinone probes electrogenerated at a microspray electrode react with the thiol functions of the pr ...

American Chemical Society2006

On-Line Counting of Cysteine Residues in Peptides during Electrospray Ionization by Electrogenerated Tags and Their Application to Protein Identification

Hubert Girault, Michel Prudent, Niels Erik Lion, Christophe Roussel, Loïc Dayon

The electrochemically induced mass spectrometric tagging of cysteines by substituted hydroquinones was studied for peptides in a classical electrospray solvent (i.e., MeOH/H2O/AcOH 50/49/1). The tagging efficiency was tested with different hydroquinone com ...

2005

Electrochemical Multi-Tagging of Cysteinyl Peptides during Microspray Mass Spectrometry: Numerical Simulation of Consecutive Reactions in a Microchannel

Hubert Girault, Loïc Dayon

On-line electrogeneration of mass tags in a microspray emitter is used to quantify the number of cysteine groups in a given peptide. A finite-element simulation of the multi-step process yields the relative distribution and concentration of tags, untagged ...

2005

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