Mathieu Benjamin Stettler

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We present a new approach for biomass assessment in cell culture using a disposable microcentrifuge tube. The specially designed tube is fitted with an upper chamber for sample loading and a lower 5 microL capillary for cell collection during centrifugatio ...

The objective of industrial cell culture technology is to produce high value-added therapeutic proteins that are well suited for the treatment of infectious diseases, cancer, and autoimmune diseases. Nowadays, an important focus is the reduction of the tim ...

NMR spectra of mammalian cells are taken using surface microprobes that are based on microfabricated planar coils. The surface microprobe resembles a miniaturized Petri dish commonly used in biological research. The diameter of the planar coils is 1 mm. Ch ...

Lactate production is monitored in industrial processes as a crucial metabolite for cultured mammalian cells. Typically lactate is strongly produced during the exponential growth phase, while its net consumption is frequently observed when cells enter into ...

NMR-microprobes based on solenoids and Helmholtz coils have been microfabricated and NMR-spectra of mammalian cells have successfully been taken. The microfabrication technology developed for these probes consists of three electroplated copper levels for l ...

A new scalable reactor was developed by applying a novel mixing principle that allows the large-scale cultivation of mammalian cells simply with surface aeration using air owing to increased liquid–gas transfer compared to standard stirred-tank bioreactors. In the cylindrical vessels (50 mL–1500 L) with a helical track attached to the inside wall, the liquid moved upward onto the track as the result of orbital shaking to increase the liquid–gas interface area significantly. This typically resulted in a 5–10

Large-scale transient gene expression in mammalian cells is being developed for the rapid production of recombinant proteins for biochemical and preclinical studies. Here, the scalability of transient production of a recombinant human antibody in Chinese h ...

Driven by the commercial success of recombinant biopharmaceuticals, there is an increasing demand for novel mammalian cell culture bioreactor systems for the rapid production of biologicals that require mammalian protein processing. Recently, orbitally sha ...

Innovative mixing principles in bioreactors, for example using the rocking of a platform to induce a backwards and forwards 'wave' , or using orbital shaking to generate a 'wave' that runs round in a cylindrical container, have proved to be successful for ...

Adeno-associated virus (AAV)-based vectors belong to the most promising gene transfer vectors in clinical studies. To provide vector for late-stage clinical trials as well as for a potential commercial phase, a scalable, cGMP-compliant process is required. ...

Mathieu Benjamin Stettler

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New disposable tubes for rapid and precise biomass assessment for suspension cultures of mammalian cells

Bioreactor processes based on disposable materials for the production of recombinant proteins from mammalian cells

NMR spectroscopy and perfusion of mammalian cells using surface microprobes

Lactate metabolism shift in CHO cell culture: the role of mitochondrial oxidative activity

Microfabricated solenoids and Helmholtz coils for NMR spectroscopy of mammalian cells

Shaken helical track bioreactors: Providing oxygen to high-density cultures of mammalian cells at volumes up to 1000L by surface aeration with air

Novel orbital shake bioreactors for transient production of CHO derived IgGs

Use of Orbital Shaken Disposable Bioreactors for Mammalian Cell Cultures from the Milliliter-Scale to the 1,000-Liter Scale

Innovative, Non-stirred Bioreactors in Scales from Milliliters up to 1000 Liters for Suspension Cultures of Cells using Disposable Bags and Containers – A Swiss Contribution

High-titer, serum-free production of adeno-associated virus vectors by polyethyleneimine-mediated plasmid transfection in mammalian suspension cells