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Lucia Baldi Unser

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Related publications (55)

Transposon mediated co-integration and co-expression of transgenes in CHO-DG44 cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Sowmya Balasubramanian

2011

Reduced glutamine concentration improves protein production in growth-arrested CHO-DG44 and HEK-293E cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Divor Kiseljak, Yashas Rajendra

For most cultivated mammalian cells, glutamine is an essential medium component. However, glutamine consumption results in the production of ammonia, a cytotoxic byproduct. Here we investigated the effect of glutamine reduction on recombinant protein produ ...

Springer Netherlands2011

Innovative, Non-stirred Bioreactors in Scales from Milliliters up to 1000 Liters for Suspension Cultures of Cells using Disposable Bags and Containers – A Swiss Contribution

Florian Maria Wurm, Lucia Baldi Unser, Mathieu Benjamin Stettler, David Hacker, Stéphanie Tissot, Gilles Broccard, Xiaowei Zhang

Innovative mixing principles in bioreactors, for example using the rocking of a platform to induce a backwards and forwards 'wave' , or using orbital shaking to generate a 'wave' that runs round in a cylindrical container, have proved to be successful for ...

Schweizerische Chemische Gesellschaft2010

Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Sarah Wulhfard

Valproic acid (VPA) is a small molecule that inhibits histone deacetylase activity. Here we report that VPA increases recombinant mRNA and protein levels in transiently transfected CHO DG44 cells. In the presence of VPA, transient recombinant antibody yiel ...

Elsevier2010

Hyperosmolarity enhances transient recombinant protein yield in Chinese hamster ovary cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Xiaowei Zhang, Isabel Fernandez Garcia

The effect of hyperosmolarity on transient recombinant protein production in Chinese hamster ovary (CHO) cells was investigated. Addition of 90 mM NaCl to the production medium ProCHO5 increased the volumetric yield of recombinant antibody up to 4-fold rel ...

Springer Netherlands2010

Respiratory syncytial virus subunit vaccine based on a recombinant fusion protein expressed transiently in mammalian cells

Florian Maria Wurm, Lucia Baldi Unser, Sophie Nallet, David Hacker

Although respiratory syncytial virus (RSV) causes severe lower respiratory tract infection in infants and adults at risk, no RSV vaccine is currently available. In this report, efforts toward the generation of an RSV subunit vaccine using recombinant RSV f ...

2009

Recombinant therapeutic protein production in cultivated mammalian cells: current status and future prospects

Florian Maria Wurm, Lucia Baldi Unser, David Hacker

Recombinant therapeutic proteins produced in mammalian cells represent a major class of biopharmaceuticals. In recent years, their demand has increased dramatically and is now driving the development of a variety of improvements to maximize their expressio ...

Elsevier2008

Sustained activation and tumor targeting of NKT cells using a CD1d-anti-HER2-scFv fusion protein induce antitumor effects in mice

Florian Maria Wurm, Lucia Baldi Unser

Invariant NKT (iNKT) cells are potent activators of DCs, NK cells, and T cells, and their antitumor activity has been well demonstrated. A single injection of the high-affinity CD1d ligand α-galactosylceramide (αGalCer) leads to short-lived iNKT cell activ ...

2008

High-titer, serum-free production of adeno-associated virus vectors by polyethyleneimine-mediated plasmid transfection in mammalian suspension cells

Florian Maria Wurm, Lucia Baldi Unser, Mathieu Benjamin Stettler

Adeno-associated virus (AAV)-based vectors belong to the most promising gene transfer vectors in clinical studies. To provide vector for late-stage clinical trials as well as for a potential commercial phase, a scalable, cGMP-compliant process is required. ...

Springer, Springer Netherlands2007

Glass reflow on 3-dimensional micro-apertures for electrophysiological measurements on-chip

Thomas Lehnert, Martinus Gijs, Lucia Baldi Unser

Springer-Verlag2007

Recombinant protein production by large-scale transient gene expression in mammalian cells : state of the art and future perspectives

Florian Maria Wurm, Lucia Baldi Unser, David Hacker

The expansion of the biologics pipeline depends on the identification of candidate proteins for clinical trials. Speed is one of the critical issues, and the rapid production of high quality, research-grade material for preclinical studies by transient gen ...

Kluwer Academic Publishers, Springer2007

Transient gene expression in suspension HEK-293 cells: application to large-scale protein production

Philippe Pierre Girard, Florian Maria Wurm, Lucia Baldi Unser, Natalie Muller

Recent advances in genomics, proteomics, and structural biology raised the general need for significant amounts of pure recombinant protein (r-protein). Because of the difficulty in obtaining in some cases proper protein folding in bacteria, several method ...

2005

Reduction of adenovirus E1A mRNA by RNAi results in enhanced recombinant protein expression in transiently transfected HEK293 cells

Florian Maria Wurm, Lucia Baldi Unser, Martin Bertschinger, David Hacker

Human embryonic kidney 293 (HEK293) cells, a widely used host for large-scale transient expression of recombinant proteins, are transformed with the adenovirus E1A and E1B genes. Because the E1A proteins function as transcriptional activators or repressors ...

2004

Transient gene expression in suspension HEK-293 cells: application to large-scale protein production

Philippe Pierre Girard, Florian Maria Wurm, Lucia Baldi Unser, David Hacker

Springer2003

Transient gene expression: A novel mammalian cell-based technology for recombinant protein production. A historical and technical perspective

Philippe Pierre Girard, Florian Maria Wurm, Martin Jordan, Jason Wright, Lucia Baldi Unser, Frédéric Grosjean, Natalie Muller, David Hacker

Springer2002

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