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Pierre Marquet

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Related publications (64)

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Quantitative phase restoration by direct inversion using the optical transfer function

Pierre Marquet, Christian Depeursinge

Quantitative phase recovery of phase objects is achieved by a direct inversion using the defocused weak object transfer function. The presented method is noniterative and is based on partially coherent principles. It also takes into account the optical pro ...

Optical Society of America2011

Time-domain optical coherence tomography with digital holographic microscopy

Pierre Marquet, Christian Depeursinge, Etienne Cuche, Florian Charrière, Pia Massatsch

We show that digital holography can be combined easily with optical coherence tomography approach. Varying the reference path length is the means used to acquire a series of holograms at different depths, providing after reconstruction images of slices at ...

2005

Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy

Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Jonas Kühn, Tristan Colomb, Benjamin Rappaz, Viesturs Simanis

Digital holography microscopy (DHM) is an optical technique which provides phase images yielding quantitative information about cell structure and cellular dynamics. Furthermore, the quantitative phase images allow the derivation of other parameters, inclu ...

SPIE Society of Photo-optical Instrumentation Engineers2009

Submicrometer tomography of cells by multiple-wavelength digital holographic microscopy in reflection

Pierre Marquet, Christian Depeursinge, Jonas Kühn, Tristan Colomb, Nicolas Pavillon, Benjamin Rappaz, Frédéric Montfort

We present first results on a method enabling mechanical scanning-free tomography with submicrometer axial resolution by multiple-wavelength digital holographic microscopy. By sequentially acquiring reflection holograms and summing 20 wavefronts equally sp ...

2009

Digital holographic microscopy applied to life sciences

Pierre Marquet, Christian Depeursinge, Jonas Kühn, Tristan Colomb, Benjamin Rappaz, Florian Charrière

Digital holographic microscopy (DHM) is applied to life sciences applications and demonstrate its capability of real-time imaging and quantitative measurements of physiological parameters such as cell volume or mean cell hemoglobin concentration (MCHC) of ...

IEEE2007

Living specimen tomography by digital holographic microscopy: morphometry of testate amoeba

Pierre Marquet, Christian Depeursinge, Tristan Colomb, Nicolas Pavillon, Benjamin Rappaz, Florian Charrière

This paper presents an optical diffraction tomography technique based on digital holographic microscopy. Quantitative 2-dimensional phase images are acquired for regularly-spaced angular positions of the specimen covering a total angle of π, allowing to bu ...

2006

Total aberrations compensation in digital holographic microscopy with a reference conjugated hologram

Pierre Marquet, Christian Depeursinge, Jonas Kühn, Tristan Colomb, Florian Charrière, Nicolas Aspert

In this paper we present a new method to achieve quantitative phase contrast imaging in Digital Holographic Microscopy (DHM) that allows to compensate for phase aberrations and image distortion by recording of a single reference hologram.We demonstrate tha ...

2006

Cell refractive index tomography by digital holographic microscopy

Pierre Marquet, Christian Depeursinge, Jonas Kühn, Tristan Colomb, Etienne Cuche, Florian Charrière, Frédéric Montfort, Mihaela Marian

For what we believe to be the first time, digital holographic microscopy is applied to perform optical diffraction tomography of a pollen grain. Transmission phase images with nanometric axial accuracy are numerically reconstructed from holograms acquired ...

2006

Real-time dual-wavelength digital holographic microscopy with a single hologram acquisition

Pierre Marquet, Christian Depeursinge, Jonas Kühn, Tristan Colomb, Etienne Cuche, Florian Charrière, Frédéric Montfort

A technique to perform two-wavelengths digital holographic microscopy (DHM) measurements with a single hologram acquisition is presented. The vertical measurement range without phase ambiguity is extended to the micron-range, thanks to the resulting synthe ...

2007

Early Cell Death Detection with Digital Holographic Microscopy

Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Nicolas Pavillon, Pascal Jourdain

Background: Digital holography provides a non-invasive measurement of the quantitative phase shifts induced by cells in culture, which can be related to cell volume changes. It has been shown previously that regulation of cell volume, in particular as it r ...

Public Library of Science2012

Exploring Neural Cell Dynamics with Digital Holographic Microscopy

Pierre Magistretti, Pierre Marquet, Christian Depeursinge

In this review, we summarize how the new concept of digital optics applied to the field of holographic microscopy has allowed the development of a reliable and flexible digital holographic quantitative phase microscopy (DH-QPM) technique at the nanoscale p ...

Annual Reviews2013

Real time, nanometric 3D-tracking of nanoparticles made possible by second harmonic generation digital holographic microscopy

Pierre Marquet, Christian Depeursinge, Etienne Shaffer

In the past decade, quantitative phase imaging gave a new dimension to optical microscopy, and the recent extension of digital holography techniques to nonlinear microscopy appears very promising, for the phase of nonlinear signal provides additional infor ...

Optical Society of America2010

Cell Morphology and Intracellular Ionic Homeostasis explored with a Multimodal Approach combining Epifluorescence and Digital Holographic Microscopy

Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Jonas Kühn, Nicolas Pavillon, Pascal Jourdain, Daniel Boss, Aleksandr Benke

The authors have developed a live-cell multimodality microscope combining epifluorescence with digital holographic microscopy; it has been implemented with a decoupling procedure allowing to separately measure from the quantitative phase important cell par ...

2010

Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy

Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Tristan Colomb, Benjamin Rappaz, Etienne Cuche

We have developed a digital holographic microscope (DHM), in a transmission mode, especially dedicated to the quantitative visualization of phase objects such as living cells. The method is based on an original numerical algorithm presented in detail elsew ...

2005

Label-free second harmonic phase imaging of biological specimen by digital holographic microscopy

Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Etienne Shaffer

We have previously developed a new way for nonscanning second-harmonic generation (SHG) microscopy [Opt. Lett. 34, 2450 (2009)]. Based on digital holography, this technique captures, in single-shot hologram acquisition, both the amplitude and the phase of ...

Optical Society of America2010

Digital holographic microscopy for study cellular dynamics

Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Benjamin Rappaz, Etienne Cuche

Based on an original numerical reconstruction algorithm (E. Cuche et al. Appl. Opt. 38, 6994 1999), we have developed a Digital Holographic Microscope (DHM), in a transmission mode, allowing to investigate noninvasively cellular structures and dynamics. DH ...

SPIE2005

WAVE FRONT SENSING METHOD AND APPARATUS

Pierre Marquet, Christian Depeursinge, Tristan Colomb, Etienne Cuche, Florian Charrière, Nicolas Aspert, Frédéric Montfort, Stéphane Bourquin, Mihaela Marian

A new way of mixing instrumental and digital means is described for the general field of wave front sensing. The present invention describes the use, the definition and the utility of digital operators, called digital wave front operators (DWFO) or ... A n ...

2006

Pierre Marquet

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Quantitative phase restoration by direct inversion using the optical transfer function

Time-domain optical coherence tomography with digital holographic microscopy

Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy

Submicrometer tomography of cells by multiple-wavelength digital holographic microscopy in reflection

Digital holographic microscopy applied to life sciences

Living specimen tomography by digital holographic microscopy: morphometry of testate amoeba

Total aberrations compensation in digital holographic microscopy with a reference conjugated hologram

Cell refractive index tomography by digital holographic microscopy

Real-time dual-wavelength digital holographic microscopy with a single hologram acquisition

Early Cell Death Detection with Digital Holographic Microscopy

Exploring Neural Cell Dynamics with Digital Holographic Microscopy

Real time, nanometric 3D-tracking of nanoparticles made possible by second harmonic generation digital holographic microscopy

Cell Morphology and Intracellular Ionic Homeostasis explored with a Multimodal Approach combining Epifluorescence and Digital Holographic Microscopy

Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy

Label-free second harmonic phase imaging of biological specimen by digital holographic microscopy

Digital holographic microscopy for study cellular dynamics

WAVE FRONT SENSING METHOD AND APPARATUS

Synergistic interaction of L-Lactate and Glycine to potentiate NMDA receptor activity: a new form of meta-potentiation?

Red blood cell structure and dynamics explored with digital holographic microscopy

Loss of Protein Tyr-phosphorylation During in vitro Storage of Human Erythrocytes: Impact on RBC Morphology