Publications by Lisa Hunt | EPFL Graph Search

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There are currently two methods for maintaining cultured mammalian cells, continuous passage at 37 degrees C and freezing in small batches. We investigated a third approach, the "pausing" of cells for days or weeks at temperatures below 37 degrees C in a v ...

Fluorescent proteins expressed in mammalian cells can be quantified quickly and noninvasively with a standard fluorescence plate reader. We have previously exploited this quality in cell growth assessment (Hunt et al., 1999b). In this work, different CHO cell lines constitutively expressing fluorescent proteins were evaluated as model systems for process development and optimization. Our results demonstrate that the fluorescence of these cell lines quickly reveals conditions that might improve the overall p

This study correlates the fluorescent signal from stable recombinant CHO cell lines expressing the green fluorescent protein (GFP) at high levels with biomass or cell number, extending the use of fluorescent proteins to applications and assays where cell growth rates are important. Using a standard fluorometer, growth of these cells can be quantified noninvasively in multiwell plates, and because signals are obtained without preparation, the same culture samples can be measured repeatedly. Even with a small

Lisa Hunt

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Low-temperature pausing of cultivated mammalian cells

Fluorescent proteins in animal cells for process development: optimization of sodium butyrate treatment as an example

GFP-expressing mammalian cells for fast, sensitive, noninvasive cell growth assessment in a kinetic mode

The use of fluorescent proteins as a tool in process development for the production of recombinant proteins by mammalian cells

Pausing of mammalian cells by cold exposure, limits and opportunities

Na+ is responsible for intra-run variations of specific productivity of CHO cell lines producing human anti RhD-IgG in bioreactors

Sodium butyrate treatment: a study of critical parameters using CHO cell lines expressing the Green Fluorescent Protein

Growth assessment using fluorescent mammalian cells