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Christian Depeursinge

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Related publications (87)

Page 2 of 5

Enhanced robustness digital holographic microscopy for demanding environment of space biology

Christian Depeursinge, Jonas Kühn, Muhammed Fatih Toy

We describe an optimized digital holographic microscopy system (DHM) suitable for high-resolution visualization of living cells under conditions of altered macroscopic mechanical forces such as those that arise from changes in gravitational force. Experime ...

2012

Quantitative phase imaging in biomedicine

Christian Depeursinge

Quantitative phase imaging (QPI) has emerged as a valuable method for investigating cells and tissues. QPI operates on unlabelled specimens and, as such, is complementary to established fluorescence microscopy, exhibiting lower phototoxicity and no photobl ...

Nature Publishing Group2018

Realistic 3D coherent transfer function inverse filtering of complex fields

Christian Depeursinge, Daniel Boss, Muhammed Fatih Toy, Yann Jérôme Michel Pascal Cotte

We present a novel technique for three-dimensional (3D) image processing of complex fields. It consists in inverting the coherent image formation by filtering the complex spectrum with a realistic 3D coherent transfer function (CTF) of a high-NA digital ho ...

Optical Society of America2011

Clinical imaging fluorescence apparatus for the endoscopic photodetection of early cancers by use of Photofrin II

Christian Depeursinge, Hubert van den Bergh, Georges Wagnières, André Studzinski

A fluorescence imaging device applied to the detection of early cancer is described. The app. is based on the imaging of laser-induced fluorescence of a dye that localizes in a tumor with a higher concn. than in the surrounding normal tissue after i.v. inj ...

Optical Society of America1997

Spatial analysis of erythrocyte membrane fluctuations by digital holographic microscopy

Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Benjamin Rappaz, Daniel Boss

Red blood cell (RBC) membrane fluctuations provide important insights into cell states. We present a spatial analysis of red blood cell membrane fluctuations by using digital holographic Microscopy (DHM). This interferometric and dye-free technique, posses ...

Elsevier2009

Complex index refraction tomography with sub ?/6-resolution

Christian Depeursinge, Nicolas Pavillon, Yann Jérôme Michel Pascal Cotte

The present invention discloses a method to improve the image resolution of a microscope. This improvement is based on the mathematical processing of the complex field computed from the measurements with a microscope of the wave emitted or scattered by the ...

2011

Shot noise influence in reconstructed phase image SNR in digital holographic microscopy

Pierre Marquet, Christian Depeursinge, Tristan Colomb, Etienne Cuche, Florian Charrière, Frédéric Montfort

In digital holographic microscopy, shot noise is an intrinsic part of the recording process with the digital camera. We present a study based on simulations and real measurements describing the shot-noise influence in the quality of the reconstructed phase ...

2006

Measurement of absolute cell volume, osmotic membrane water permeability, and refractive index of transmembrane water and solute flux by digital holographic microscopy

Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Pascal Jourdain, Daniel Boss

A dual-wavelength digital holographic microscope to measure absolute volume of living cells is proposed. The optical setup allows us to reconstruct two quantitative phase contrast images at two different wavelengths from a single hologram acquisition. When ...

SPIE Society of Photo-optical Instrumentation Engineers2013

Sub-Rayleigh resolution by phase imaging

Christian Depeursinge, Nicolas Pavillon, Etienne Shaffer, Muhammed Fatih Toy, Yann Jérôme Michel Pascal Cotte

We report the experimental observation of systematically occurring phase singularities in coherent imaging of sub-Rayleigh distanced objects. A theory that relates the observation to the sub-Rayleigh distance is presented and compared with experimental mea ...

Optical Society of America2010

Nanoindentation and birefringence measurements on fused silica specimen exposed to low-energy femtosecond pulses

Christian Depeursinge, Yves Bellouard, Tristan Colomb

Femtosecond laser pulses used in a regime below the ablation threshold have two noticeable effects on Fused Silica (a-SiO2): they locally increase the material refractive index and modify its HF etching selectivity. The nature of the structural changes ind ...

2006

Spatially-Resolved Eigenmode Decomposition of Red Blood Cells Membrane Fluctuations Questions the Role of ATP in Flickering

Dimitri Nestor Alice Van De Ville, Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Benjamin Rappaz, Daniel Boss

Red blood cells (RBCs) present unique reversible shape deformability, essential for both function and survival, resulting notably in cell membrane fluctuations (CMF). These CMF have been subject of many studies in order to obtain a better understanding of ...

Public Library Science2012

Cell Morphology and Intracellular Ionic Homeostasis explored with a Multimodal Approach combining Epifluorescence and Digital Holographic Microscopy

Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Jonas Kühn, Nicolas Pavillon, Pascal Jourdain, Daniel Boss, Aleksandr Benke

The authors have developed a live-cell multimodality microscope combining epifluorescence with digital holographic microscopy; it has been implemented with a decoupling procedure allowing to separately measure from the quantitative phase important cell par ...

2010

Submicrometer tomography of cells by multiple-wavelength digital holographic microscopy in reflection

Pierre Marquet, Christian Depeursinge, Jonas Kühn, Tristan Colomb, Nicolas Pavillon, Benjamin Rappaz, Frédéric Montfort

We present first results on a method enabling mechanical scanning-free tomography with submicrometer axial resolution by multiple-wavelength digital holographic microscopy. By sequentially acquiring reflection holograms and summing 20 wavefronts equally sp ...

2009

Quantitative phase noise in a two-color low coherence Digital Holographic Microscope

Christian Depeursinge, Christophe Moser, Frédéric Montfort, Zahra Monemhaghdoust

In digital holographic microscopy (DHM), the long coherence length of laser light causes parasitic interferences due to multiple reflections in and by optical components in the optical path of the microscope and thus degrades the image quality. The parasit ...

Spie-Int Soc Optical Engineering2013

Off-axis digital holographic camera for quantitative phase microscopy

Christophe Moser, Christian Depeursinge, Frédéric Montfort, Zahra Monemhaghdoust

We propose and experimentally demonstrate a digital holographic camera which can be attached to the camera port of a conventional microscope for obtaining digital holograms in a self-reference configuration, under short coherence illumination and in a sing ...

Optical Soc Amer2014

On the complex three-dimensional amplitude point spread function of lenses and microscope objectives: theoretical aspects, simulations and measurements by digital holography

Pierre Marquet, Christian Depeursinge, Jonas Kühn, Tristan Colomb, Florian Charrière, Frédéric Montfort, Mihaela Marian

The point spread function is widely used to characterize the three-dimensional imaging capabilities of an optical system. Usually, attention is paid only to the intensity point spread function, whereas the phase point spread function is most often neglecte ...

2007

Real time, nanometric 3D-tracking of nanoparticles made possible by second harmonic generation digital holographic microscopy

Pierre Marquet, Christian Depeursinge, Etienne Shaffer

In the past decade, quantitative phase imaging gave a new dimension to optical microscopy, and the recent extension of digital holography techniques to nonlinear microscopy appears very promising, for the phase of nonlinear signal provides additional infor ...

Optical Society of America2010

Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy

Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Jonas Kühn, Tristan Colomb, Benjamin Rappaz, Viesturs Simanis

Digital holography microscopy (DHM) is an optical technique which provides phase images yielding quantitative information about cell structure and cellular dynamics. Furthermore, the quantitative phase images allow the derivation of other parameters, inclu ...

SPIE Society of Photo-optical Instrumentation Engineers2009

Marker-free phase nanoscopy

Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Nicolas Pavillon, Pascal Jourdain, Daniel Boss, Muhammed Fatih Toy, Yann Jérôme Michel Pascal Cotte

We introduce a microscopic method that determines quantitative optical properties beyond the optical diffraction limit and allows direct imaging of unstained living biological specimens. In established holographic microscopy, complex fields are measured us ...

Nature Publishing Group2013

Total aberrations compensation in digital holographic microscopy with a reference conjugated hologram

Pierre Marquet, Christian Depeursinge, Jonas Kühn, Tristan Colomb, Florian Charrière, Nicolas Aspert

In this paper we present a new method to achieve quantitative phase contrast imaging in Digital Holographic Microscopy (DHM) that allows to compensate for phase aberrations and image distortion by recording of a single reference hologram.We demonstrate tha ...

2006