Publications by Arnaud Comment

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BackgroundThe development of nanoscale secondary ion mass spectrometry (NanoSIMS) has revolutionized the study of biological tissues by enabling, e.g., the visualization and quantification of metabolic processes at subcellular length scales. However, the a ...

Hyperpolarized γ-Glu-[1-13C]Gly provides a non-invasive means to detect γ-glutamyl transpeptidase (GGT) enzyme activity in vivo with potential for application in functional imaging. Since GGT is most abundant in the proximal tubules of the kidney, and since the properties of γ-Glu-[1-13C]Gly are suitable for in vivo hyperpolarized 13C metabolic analysis, it was proposed as a molecular probe to study kidney function. The aim of the present study is to identify the dose of γ-Glu-[1-13C]Gly that gives high NMR

The dynamics of L-lactate transport across the blood-brain barrier (BBB) and its cerebral metabolism are still subject to debate. We studied lactate uptake and intracellular metabolism in the mouse brain using hyperpolarized C-13 magnetic resonance spectro ...

Under normal conditions, the heart mainly relies on fatty acid oxidation to meet its energy needs. Changes in myocardial fuel preference are noted in the diseased and failing heart. The magnetic resonance signal enhancement provided by spin hyperpolarizati ...

Magnetic resonance elastography (MRE) is a powerful technique to assess the mechanical properties of living tissue. However, it suffers from reduced sensitivity in regions with short T-2 and T-2* such as in tissue with high concentrations of paramagnetic i ...

For most of the last forty years, the techniques of Dynamic Nuclear Polarization (DNP) have been confined to particle-physics laboratories building polarized targets, but recently it has been shown that samples similar to a solid target can be transformed ...

In standard Dynamic Nuclear Polarization (DNP) electron spins are polarized at low temperatures in a strong magnetic field and this polarization is transferred to the nuclear spins by means of a microwave field. To obtain high nuclear polarizations cryogen ...

BACKGROUND: The heart relies on continuous energy production and imbalances herein impair cardiac function directly. The tricarboxylic acid (TCA) cycle is the primary means of energy generation in the healthy myocardium, but direct noninvasive quantificati ...

Hyperpolarized substrates prepared via dissolution dynamic nuclear polarization have been proposed as magnetic resonance imaging (MRI) agents for cancer or cardiac failure diagnosis and therapy monitoring through the detection of metabolic impairments in v ...

Pyruvate membrane crossing and its lactate dehydrogenase-mediated conversion to lactate in cells featuring different levels of expression of membrane monocarboxylate transporters (MCT4) were probed by dissolution dynamic nuclear polarization-enhanced NMR. ...

The mammalian brain relies primarily on glucose as a fuel to meet its high metabolic demand. Among the various techniques used to study cerebral metabolism, C-13 magnetic resonance spectroscopy (MRS) allows following the fate of C-13-enriched substrates th ...

The tremendous polarization enhancement afforded by dissolution dynamic nuclear polarization (DNP) can be taken advantage of to perform preclinical in vivo molecular and metabolic imaging. Following the injection of molecules that are hyperpolarized via di ...

New low-temperature inserts compatible with an existing hyperpolarizer were developed to dynamically polarize nuclei in large samples. The performance of the system was tested on 8 ml glassy frozen solutions containing 13C-labeled molecules and doped with ...

Hyperpolarized molecular probes can be effectively used as pH markers. To date, the only two probes reported in vivo as extracellular pH sensors are 13C-bicarbonate, and [1,5-13C2]zymonic acid. Alaninamide is a derivative of alanine which is found to be sensitive to variations of pH in the physiological range. The aim of the present study was to assess the feasibility of using alaninamide as a pH probe in vivo. The alaninamide titration curve was determined by performing 13C NMR measurements at 9.4 T, 37° o

In recent years methods of creating 'hyperpolarized' substances have gained considerable attention in biomedical magnetic resonance and dynamic nuclear polarization (DNP) is one of the most promising, especially for imaging applications. Here we present re ...

The advent of dissolution dynamic nuclear polarization (DNP) led to the emergence of a new kind of magnetic resonance (MR) measurements providing the opportunity to probe metabolism in vivo in real time. It has been shown that, following the injection of h ...

Hyperpolarized carbon-13 magnetic resonance spectroscopy ((13)C MRS) enables the sensitive and noninvasive assessment of the metabolic changes occurring during myocardial ischemia-reperfusion. Ischemia-reperfusion models using hyperpolarized (13)C MRS are ...

The Krebs (or tricarboxylic acid (TCA)) cycle has a central role in the regulation of brain energy regulation and metabolism, yet brain TCA cycle intermediates have never been directly detected in vivo. This study reports the first direct in vivo observati ...

An infusion pump comprising a substance and pushing fluid inlet, a substance outlet, a central chamber communicating with said inlet and said outlet, and injecting means for injecting a substance into a biological object, wherein the infusion pump further ...

Lactate plays an important role in brain energetics and is essential for neuronal function. Alteration of its metabolism might be associated with mechanisms linked to neurodegenerative disorders1. The purpose of this study was to demonstrate the potential of of hyperpolarized [1-13C] lactate to sudy brain metabolism in mice. Following injection, hyperpolarized [1-13C] lactate is transferred through the blood brain barrier (BBB) via monocarboxylate transporters (MCTs), in particular MCT12. These transporters

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Correlated cryo-SEM and CryoNanoSIMS imaging of biological tissue

Assessing γ-glutamyl transpeptidase activity in kidney using hyperpolarized γ-Glu-[1-13C]Gly

Hyperpolarized C-13 Magnetic Resonance Spectroscopy Reveals the Rate-Limiting Role of the Blood-Brain Barrier in the Cerebral Uptake and Metabolism of L-Lactate in Vivo

Detection of myocardial medium‐chain fatty acid oxidation and tricarboxylic acid cycle activity with hyperpolarized [1– 13 C]octanoate

Overhauser-enhanced magnetic resonance elastography

Design and performance of a DNP prepolarizer coupled to a rodent MRI scanner

An apparatus for pulsed ESR and DNP experiments using optically excited triplet states down to liquid helium temperatures

Direct noninvasive estimation of myocardial tricarboxylic acid cycle flux in vivo using hyperpolarized 13C magnetic resonance

Hyperpolarization without persistent radicals for in vivo real-time metabolic imaging

Pyruvate cellular uptake and enzymatic conversion probed by dissolution DNP-NMR: the impact of overexpressed membrane transporters

Measuring glucose cerebral metabolism in the healthy mouse using hyperpolarized C-13 magnetic resonance

Dissolution DNP for in vivo preclinical studies

Producing over 100 ml of highly concentrated hyperpolarized solution by means of dissolution DNP

Probing renal pH using hyperpolarized [1-13C]alaninamide

Dynamic nuclear polarization of small labelled molecules in frozen water-alcohol solutions

Hyperpolarized MRS: New tool to study real-time brain function and metabolism

An Intact Small Animal Model of Myocardial Ischemia-Reperfusion: Characterization of Metabolic Changes by Hyperpolarized 13C MR Spectroscopy

In vivo detection of brain Krebs cycle intermediate by hyperpolarized magnetic resonance

Infusion pump with phase separator

Lactate brain metabolism in different mouse strains probed by hyperpolarized 13C MRS