Publications by Tristan Colomb

Related publications (27)

Submicrometer optical tomography by multiple-wavelength digital holographic microscopy

Pierre Marquet, Christian Depeursinge, Jonas Kühn, Tristan Colomb, Etienne Cuche, Florian Charrière, Frédéric Montfort

We present a method for submicrometer tomographic imaging using multiple wavelengths in digital holographic microscopy. This method is based on the recording, at different wavelengths equally separated in the k domain, in off- axis geometry, of the interfe ...

2006

Purely numerical compensation for microscope objective phase curvature in digital holographic microscopy: influence of digital phase mask position

Pierre Marquet, Christian Depeursinge, Tristan Colomb, Etienne Cuche, Florian Charrière, Frédéric Montfort

Introducing a microscope objective in an interferometric setup induces a phase curvature on the resulting wavefront. In digital holography, the compensation of this curvature is often done by introducing an identical curvature in the reference arm and the ...

2006

Polarization microscopy by use of digital holography: application to optical- fiber birefringence measurements

René Salathé, Hans Georg Limberger, Pierre Marquet, Christian Depeursinge, Tristan Colomb, Etienne Cuche, Florian Dürr

We present a digital holographic microscope that permits one to image polarization state. This technique results from the coupling of digital holographic microscopy and polarization digital holography. The interference between two orthogonally polarized re ...

2005

Characterization of microlenses by digital holographic microscopy

Pierre Marquet, Christian Depeursinge, Jonas Kühn, Tristan Colomb, Etienne Cuche, Florian Charrière, Frédéric Montfort

We demonstrate the use of digital holographic microscopy (DHM) as a metrological tool in micro-optics testing. Measurement principles are compared with those performed with Twyman-Green, Mach-Zehnder, and white-light interferometers. Measurements performed ...

2006

Influence of shot noise on phase measurement accuracy in digital holographic microscopy

Pierre Marquet, Christian Depeursinge, Jonas Kühn, Tristan Colomb, Benjamin Rappaz, Florian Charrière

Digital Holographic Microscopy (DHM) is a single shot interferometric technique, which provides quantitative phase images with subwavelength axial accuracy. A short hologram acquisition time (down to microseconds), allows DHM to offer a reduced sensitivity ...

2007

Shot noise influence in reconstructed phase image SNR in digital holographic microscopy

Pierre Marquet, Christian Depeursinge, Tristan Colomb, Etienne Cuche, Florian Charrière, Frédéric Montfort

In digital holographic microscopy, shot noise is an intrinsic part of the recording process with the digital camera. We present a study based on simulations and real measurements describing the shot-noise influence in the quality of the reconstructed phase ...

2006

Nanoindentation and birefringence measurements on fused silica specimen exposed to low-energy femtosecond pulses

Christian Depeursinge, Yves Bellouard, Tristan Colomb

Femtosecond laser pulses used in a regime below the ablation threshold have two noticeable effects on Fused Silica (a-SiO2): they locally increase the material refractive index and modify its HF etching selectivity. The nature of the structural changes ind ...

2006

Submicrometer tomography of cells by multiple-wavelength digital holographic microscopy in reflection

Pierre Marquet, Christian Depeursinge, Jonas Kühn, Tristan Colomb, Nicolas Pavillon, Benjamin Rappaz, Frédéric Montfort

We present first results on a method enabling mechanical scanning-free tomography with submicrometer axial resolution by multiple-wavelength digital holographic microscopy. By sequentially acquiring reflection holograms and summing 20 wavefronts equally sp ...

2009

Numerical aberrations compensation and polarization imaging in digital holographic microscopy

Tristan Colomb

In this thesis, we describe a method for the numerical reconstruction of the complete wavefront properties from a single digital hologram: the amplitude, the phase and the polarization state. For this purpose, we present the principle of digital holographi ...

EPFL2006

On the complex three-dimensional amplitude point spread function of lenses and microscope objectives: theoretical aspects, simulations and measurements by digital holography

Pierre Marquet, Christian Depeursinge, Jonas Kühn, Tristan Colomb, Florian Charrière, Frédéric Montfort, Mihaela Marian

The point spread function is widely used to characterize the three-dimensional imaging capabilities of an optical system. Usually, attention is paid only to the intensity point spread function, whereas the phase point spread function is most often neglecte ...

2007

Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy

Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Jonas Kühn, Tristan Colomb, Benjamin Rappaz, Viesturs Simanis

Digital holography microscopy (DHM) is an optical technique which provides phase images yielding quantitative information about cell structure and cellular dynamics. Furthermore, the quantitative phase images allow the derivation of other parameters, inclu ...

SPIE Society of Photo-optical Instrumentation Engineers2009

Total aberrations compensation in digital holographic microscopy with a reference conjugated hologram

Pierre Marquet, Christian Depeursinge, Jonas Kühn, Tristan Colomb, Florian Charrière, Nicolas Aspert

In this paper we present a new method to achieve quantitative phase contrast imaging in Digital Holographic Microscopy (DHM) that allows to compensate for phase aberrations and image distortion by recording of a single reference hologram.We demonstrate tha ...

2006

Amplitude point spread function measurement of high NA microscope objectives by digital holographic microscopy

Pierre Marquet, Christian Depeursinge, Tristan Colomb, Florian Charrière, Mihaela Marian

We present here a three-dimensional evaluation of the amplitude point-spread function (APSF) of a microscope objective (MO), based on a single holographic acquisition of its pupil wavefront. The aberration function is extracted from this pupil measurements ...

2007

Real-time dual-wavelength digital holographic microscopy with a single hologram acquisition

Pierre Marquet, Christian Depeursinge, Jonas Kühn, Tristan Colomb, Etienne Cuche, Florian Charrière, Frédéric Montfort

A technique to perform two-wavelengths digital holographic microscopy (DHM) measurements with a single hologram acquisition is presented. The vertical measurement range without phase ambiguity is extended to the micron-range, thanks to the resulting synthe ...

2007

Living specimen tomography by digital holographic microscopy: morphometry of testate amoeba

Pierre Marquet, Christian Depeursinge, Tristan Colomb, Nicolas Pavillon, Benjamin Rappaz, Florian Charrière

This paper presents an optical diffraction tomography technique based on digital holographic microscopy. Quantitative 2-dimensional phase images are acquired for regularly-spaced angular positions of the specimen covering a total angle of π, allowing to bu ...

2006

Digital holographic microscopy applied to life sciences

Pierre Marquet, Christian Depeursinge, Jonas Kühn, Tristan Colomb, Benjamin Rappaz, Florian Charrière

Digital holographic microscopy (DHM) is applied to life sciences applications and demonstrate its capability of real-time imaging and quantitative measurements of physiological parameters such as cell volume or mean cell hemoglobin concentration (MCHC) of ...

IEEE2007

Versatile spectral modulation of a broadband source for digital holographic microscopy

Christophe Moser, Tristan Colomb, Gaël Nardin

We demonstrate the potential of spatial light modulators for the spectral control of a broadband source in digital holographic microscopy. Used in a ‘pulse-shaping’ geometry, the spatial light modulator provides a versatile control over the bandwidth and w ...

Optical Society of America2016

Numerical parametric lens for shifting, magnification and complete aberration compensation in digital holographic microscopy

Pierre Marquet, Christian Depeursinge, Jonas Kühn, Tristan Colomb, Etienne Cuche, Florian Charrière, Nicolas Aspert, Frédéric Montfort, Stéphane Bourquin, Mihaela Marian

The concept of numerical parametric lenses (NPL) is introduced to achieve wavefront reconstruction in digital holography. It is shown that operations usually performed by optical components and described in ray geometrical optics, such as image shifting, m ...

2006

Method and apparatus to measure and compute the amplitude point spread function and associated parameters of a coherent optical imaging system

Pierre Marquet, Christian Depeursinge, Tristan Colomb, Florian Charrière, Mihaela Marian

The invention concerns a method of and an apparatus for the measurement of the Amplitude Point Spread Function (APSF), of an Optical Imaging System (OIS), wherein the APSF is defined as the complex field distribution of the beam delivered by the OIS and or ...

2008

Digital holographic reflectometry

Christian Depeursinge, Jonas Kühn, Tristan Colomb, Nicolas Pavillon, Ahmet Ata Akatay

Digital holographic microscopy (DHM) is an interferometric technique that allows real-time imaging of the entire complex optical wavefront (amplitude and phase) reflected by or transmitted through a sample. To our knowledge, only the quantitative phase is ...

2010