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This lecture explores the essential role of vitamins in maintaining a healthy gut microbiome, discussing the symbiotic relationship between humans and beneficial microbes, the impact of diet on gut microbiota, and the synthesis of B-vitamins by the human gut microbiome. It also delves into the modulation of the microbiome through vitamin supplementation, the effects of vitamin B2, B3, B6, and B9 on microbial abundance, and the potential benefits of colon-delivered vitamins in enhancing gut health.