Restriction enzymeA restriction enzyme, restriction endonuclease, REase, ENase or restrictase is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. Restriction enzymes are one class of the broader endonuclease group of enzymes. Restriction enzymes are commonly classified into five types, which differ in their structure and whether they cut their DNA substrate at their recognition site, or if the recognition and cleavage sites are separate from one another.
Restriction digestA restriction digest is a procedure used in molecular biology to prepare DNA for analysis or other processing. It is sometimes termed DNA fragmentation, though this term is used for other procedures as well. In a restriction digest, DNA molecules are cleaved at specific restriction sites of 4-12 nucleotides in length by use of restriction enzymes which recognize these sequences. The resulting digested DNA is very often selectively amplified using polymerase chain reaction (PCR), making it more suitable for analytical techniques such as agarose gel electrophoresis, and chromatography.
Restriction fragment length polymorphismIn molecular biology, restriction fragment length polymorphism (RFLP) is a technique that exploits variations in homologous DNA sequences, known as polymorphisms, populations, or species or to pinpoint the locations of genes within a sequence. The term may refer to a polymorphism itself, as detected through the differing locations of restriction enzyme sites, or to a related laboratory technique by which such differences can be illustrated.
Restriction siteRestriction sites, or restriction recognition sites, are located on a DNA molecule containing specific (4-8 base pairs in length) sequences of nucleotides, which are recognized by restriction enzymes. These are generally palindromic sequences (because restriction enzymes usually bind as homodimers), and a particular restriction enzyme may cut the sequence between two nucleotides within its recognition site, or somewhere nearby. For example, the common restriction enzyme EcoRI recognizes the palindromic sequence GAATTC and cuts between the G and the A on both the top and bottom strands.
Injective functionIn mathematics, an injective function (also known as injection, or one-to-one function) is a function f that maps distinct elements of its domain to distinct elements; that is, x1 ≠ x2 implies f(x1) f(x2). (Equivalently, f(x1) = f(x2) implies x1 = x2 in the equivalent contrapositive statement.) In other words, every element of the function's codomain is the of one element of its domain. The term must not be confused with that refers to bijective functions, which are functions such that each element in the codomain is an image of exactly one element in the domain.
Injective objectIn mathematics, especially in the field of , the concept of injective object is a generalization of the concept of injective module. This concept is important in cohomology, in homotopy theory and in the theory of . The dual notion is that of a projective object. An in a is said to be injective if for every monomorphism and every morphism there exists a morphism extending to , i.e. such that . That is, every morphism factors through every monomorphism . The morphism in the above definition is not required to be uniquely determined by and .
Injective moduleIn mathematics, especially in the area of abstract algebra known as module theory, an injective module is a module Q that shares certain desirable properties with the Z-module Q of all rational numbers. Specifically, if Q is a submodule of some other module, then it is already a direct summand of that module; also, given a submodule of a module Y, any module homomorphism from this submodule to Q can be extended to a homomorphism from all of Y to Q. This concept is to that of projective modules.
