Lecture

Biosensors and Electronic Biochips

Graph Chat

Chat with Graph Search

Ask any question about EPFL courses, lectures, exercises, research, news, etc. or try the example questions below.

DISCLAIMER: The Graph Chatbot is not programmed to provide explicit or categorical answers to your questions. Rather, it transforms your questions into API requests that are distributed across the various IT services officially administered by EPFL. Its purpose is solely to collect and recommend relevant references to content that you can explore to help you answer your questions.

In course

EE-515: Fundamentals of biosensors and electronic biochips

The labels "biosensor" and "eBiochip" have been employed to refer to the most diverse systems and in several fields of application. The course is meant not only to provide means to dig into this sea

Description

This lecture covers the definition and working rationale of biosensors and electronic biochips, focusing on the detection of molecules on surfaces, examples of biochips, features describing a biosensor, output-measurand relationship, sensitivity, calibration curve, limit of detection, resolution, analyte selectivity, selectivity-specificity, prevention of non-specific adsorption, repeatability-reproducibility, hysteresis, precision-accuracy, sensor characterization, overload characteristics, and ambient conditions. The instructor discusses the tools used for bioanalytics, such as measuring physiological parameters and quantifying DNA/RNA content in samples, as well as advanced platforms like microarrays and microcantilevers.

Instructor

Carlotta Guiducci

Teaching & PhD PhD Students Micaela Siria Cristofori, Edoardo Bargis, Yide Jiao, Seyyed Hamid Sadat Mansoury Past EPFL PhD Students Giulia Cappi, Enrico Accastelli, Anna Ferretti, Enrica Rollo, Samuel Kilchenmann, Elena-Diana Bojescu, Kevin Keim, Pierre-Emmanuel Marie Thiriet, Saurabh Tomar, Gloria Porro, Till Jonathan Ryser Past EPFL PhD Students as codirector Yüksel Temiz Courses Electrical systems and electronics I EE-295 The course has the aim to provide the fundamental knowledge and e

Official source

Related concepts (12)

DNA microarray

A DNA microarray (also commonly known as DNA chip or biochip) is a collection of microscopic DNA spots attached to a solid surface. Scientists use DNA microarrays to measure the expression levels of large numbers of genes simultaneously or to genotype multiple regions of a genome. Each DNA spot contains picomoles (10−12 moles) of a specific DNA sequence, known as probes (or reporters or oligos). These can be a short section of a gene or other DNA element that are used to hybridize a cDNA or cRNA (also called anti-sense RNA) sample (called target) under high-stringency conditions.

Microarray

A microarray is a multiplex lab-on-a-chip. Its purpose is to simultaneously detect the expression of thousands of biological interactions. It is a two-dimensional array on a solid substrate—usually a glass slide or silicon thin-film cell—that assays (tests) large amounts of biological material using high-throughput screening miniaturized, multiplexed and parallel processing and detection methods. The concept and methodology of microarrays was first introduced and illustrated in antibody microarrays (also referred to as antibody matrix) by Tse Wen Chang in 1983 in a scientific publication and a series of patents.

Protein microarray

A protein microarray (or protein chip) is a high-throughput method used to track the interactions and activities of proteins, and to determine their function, and determining function on a large scale. Its main advantage lies in the fact that large numbers of proteins can be tracked in parallel. The chip consists of a support surface such as a glass slide, nitrocellulose membrane, bead, or microtitre plate, to which an array of capture proteins is bound. Probe molecules, typically labeled with a fluorescent dye, are added to the array.

Complementary DNA

In genetics, complementary DNA (cDNA) is DNA synthesized from a single-stranded RNA (e.g., messenger RNA (mRNA) or microRNA (miRNA)) template in a reaction catalyzed by the enzyme reverse transcriptase. cDNA is often used to express a specific protein in a cell that does not normally express that protein (i.e., heterologous expression), or to sequence or quantify mRNA molecules using DNA based methods (qPCR, RNA-seq). cDNA that codes for a specific protein can be transferred to a recipient cell for expression, often bacterial or yeast expression systems.

Molecular biology

Molecular biology məˈlɛkjʊlər is the study of chemical and physical structure of biological macromolecules. It is a branch of biology that seeks to understand the molecular basis of biological activity in and between cells, including biomolecular synthesis, modification, mechanisms, and interactions. Molecular biology was first described as an approach focused on the underpinnings of biological phenomena—uncovering the structures of biological molecules as well as their interactions, and how these interactions explain observations of classical biology.

Related lectures (6)

Microsystems for Biology: Nanopores and DNA Analysis

Delves into microsystems for biology, highlighting the use of nanopores for DNA analysis.

DNA & Protein Arrays

Explores DNA array applications, fabrication methods, SNP detection, protein array usage, array patterning, and microarray vs. RNA-Seq comparison.

Genomic Data Analysis: Techniques and Applications

Covers gene expression measurement techniques and analysis methods in genomics.

DNA Analysis Techniques

Explores the significance of DNA analysis techniques in healthcare and food industry.

Linear Regression: Basics