Lecture

Microbial Growth and Stoichiometry

Graph Chat

Chat with Graph Search

Ask any question about EPFL courses, lectures, exercises, research, news, etc. or try the example questions below.

DISCLAIMER: The Graph Chatbot is not programmed to provide explicit or categorical answers to your questions. Rather, it transforms your questions into API requests that are distributed across the various IT services officially administered by EPFL. Its purpose is solely to collect and recommend relevant references to content that you can explore to help you answer your questions.

In course

ChE-311: Biochemical engineering

This course introduces the basic principles of bioprocess engineering and highlights the similarities and differences with chemical engineering. Without going into the fundamentals, it proposes an overview of the techniques for fermentation as well as prod ...

Description

This lecture explores the complex processes of cell growth and product formation, focusing on the kinetics and stoichiometry of intracellular reactions. It delves into the law of the minimum, stoichiometric models, medium evaluation, elemental balances, and energy yields in biochemical engineering. The instructor discusses the relationship between growth rates of biomass and substrate uptake, emphasizing the importance of stoichiometry in exponential cultures. Various microbial growth media types and their functions are examined, along with the elemental composition of cells. The lecture also covers the theoretical approach to designing a medium, systematics of elemental balancing for microbial reactions, and the degree of reduction in compounds. Additionally, it addresses the maximum possible yield, energy balance, and heat of reaction in processes with biomass production.

Instructor

Simon Crelier

Teaching & PhD Courses Biochemical engineering ChE-311 This course introduces the basic principles of bioprocess engineering and highlights the similarities and differences with chemical engineering. Without going into the fundamentals, it proposes an overview of the techniques for fermentation as well as product purification (DownStream Processing) Bioprocesses and downstream processing ChE-437 This course aims at a more advanced coverage of the basic aspects discussed in m

Official source

Related concepts (32)

Cell growth

Cell growth refers to an increase in the total mass of a cell, including both cytoplasmic, nuclear and organelle volume. Cell growth occurs when the overall rate of cellular biosynthesis (production of biomolecules or anabolism) is greater than the overall rate of cellular degradation (the destruction of biomolecules via the proteasome, lysosome or autophagy, or catabolism). Cell growth is not to be confused with cell division or the cell cycle, which are distinct processes that can occur alongside cell growth during the process of cell proliferation, where a cell, known as the mother cell, grows and divides to produce two daughter cells.

Growth factor

A growth factor is a naturally occurring substance capable of stimulating cell proliferation, wound healing, and occasionally cellular differentiation. Usually it is a secreted protein or a steroid hormone. Growth factors are important for regulating a variety of cellular processes. Growth factors typically act as signaling molecules between cells. Examples are cytokines and hormones that bind to specific receptors on the surface of their target cells. They often promote cell differentiation and maturation, which varies between growth factors.

Cell surface receptor

Cell surface receptors (membrane receptors, transmembrane receptors) are receptors that are embedded in the plasma membrane of cells. They act in cell signaling by receiving (binding to) extracellular molecules. They are specialized integral membrane proteins that allow communication between the cell and the extracellular space. The extracellular molecules may be hormones, neurotransmitters, cytokines, growth factors, cell adhesion molecules, or nutrients; they react with the receptor to induce changes in the metabolism and activity of a cell.

Epidermal growth factor

Epidermal growth factor (EGF) is a protein that stimulates cell growth and differentiation by binding to its receptor, EGFR. Human EGF is 6-kDa and has 53 amino acid residues and three intramolecular disulfide bonds. EGF was originally described as a secreted peptide found in the submaxillary glands of mice and in human urine. EGF has since been found in many human tissues, including platelets, submandibular gland (submaxillary gland), and parotid gland. Initially, human EGF was known as urogastrone.

Cell proliferation

Cell proliferation is the process by which a cell grows and divides to produce two daughter cells. Cell proliferation leads to an exponential increase in cell number and is therefore a rapid mechanism of tissue growth. Cell proliferation requires both cell growth and cell division to occur at the same time, such that the average size of cells remains constant in the population. Cell division can occur without cell growth, producing many progressively smaller cells (as in cleavage of the zygote), while cell growth can occur without cell division to produce a single larger cell (as in growth of neurons).

Related lectures (52)

Continuous Culture: General Mass Balance

Explores continuous culture using chemostats and plug flow reactors, covering mass balance equations, ideal chemostat operation, and batch vs. continuous culture.

Mechanobiology of Cell Growth

Explores the mechanobiology of cell growth, focusing on essential components, force curve-based sample characterization, and the impact of turgor pressure on growth.

Biomass Conversion: Thermochemical Processes

Explores the thermochemical conversion of biomass into biofuels and chemicals.

Sustained Proliferation: RTK/PI3K/mTOR Pathway in Cancer

Explores the RTK/PI3K/mTOR pathway in cancer, inhibitors, completed trials, and effects of Akt/PKB on survival and proliferation.

Irreducible Polynomials: Degree and Roots