Lecture

Polymerase Chain Reaction: Basics and Process

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In course

BIO-109: Introduction to life sciences (for IC)

Ce cours présente les principes fondamentaux à l'œuvre dans les organismes vivants. Autant que possible, l'accent est mis sur les contributions de l'Informatique aux progrès des Sciences de la Vie. ...

Description

This lecture covers the basics and process of Polymerase Chain Reaction (PCR), including denaturation, annealing, and extension steps, as well as the role of polymerase, primers, and free nucleotides in DNA replication. The lecture also discusses the collaboration involved in creating the content.

Instructor

Romain Zufferey

Teaching & PhD Courses Biology for engineers MSE-212 This course consists of an introduction to biology and more particularly to biology as a multidisciplinary field, emphasizing natural examples of materials engineering. It should therefore allow engineering students to find inspiration from biology in future materials research problems. Cellular biology and biochemistry for engineers BIO-105 Basic course in biochemistry as well as cellular and molecular biology for non-life

Official source

Related concepts (29)

Real-time polymerase chain reaction

A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR. Real-time PCR can be used quantitatively and semi-quantitatively (i.e., above/below a certain amount of DNA molecules).

Reverse transcription polymerase chain reaction

Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique combining reverse transcription of RNA into DNA (in this context called complementary DNA or cDNA) and amplification of specific DNA targets using polymerase chain reaction (PCR). It is primarily used to measure the amount of a specific RNA. This is achieved by monitoring the amplification reaction using fluorescence, a technique called real-time PCR or quantitative PCR (qPCR).

Origin of replication

The origin of replication (also called the replication origin) is a particular sequence in a genome at which replication is initiated. Propagation of the genetic material between generations requires timely and accurate duplication of DNA by semiconservative replication prior to cell division to ensure each daughter cell receives the full complement of chromosomes. This can either involve the replication of DNA in living organisms such as prokaryotes and eukaryotes, or that of DNA or RNA in viruses, such as double-stranded RNA viruses.

Field extension

In mathematics, particularly in algebra, a field extension is a pair of fields such that the operations of K are those of L restricted to K. In this case, L is an extension field of K and K is a subfield of L. For example, under the usual notions of addition and multiplication, the complex numbers are an extension field of the real numbers; the real numbers are a subfield of the complex numbers. Field extensions are fundamental in algebraic number theory, and in the study of polynomial roots through Galois theory, and are widely used in algebraic geometry.

Abelian extension

In abstract algebra, an abelian extension is a Galois extension whose Galois group is abelian. When the Galois group is also cyclic, the extension is also called a cyclic extension. Going in the other direction, a Galois extension is called solvable if its Galois group is solvable, i.e., if the group can be decomposed into a series of normal extensions of an abelian group. Every finite extension of a finite field is a cyclic extension.

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