DNA sequencingDNA sequencing is the process of determining the nucleic acid sequence – the order of nucleotides in DNA. It includes any method or technology that is used to determine the order of the four bases: adenine, guanine, cytosine, and thymine. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery. Knowledge of DNA sequences has become indispensable for basic biological research, DNA Genographic Projects and in numerous applied fields such as medical diagnosis, biotechnology, forensic biology, virology and biological systematics.
SequencingIn genetics and biochemistry, sequencing means to determine the primary structure (sometimes incorrectly called the primary sequence) of an unbranched biopolymer. Sequencing results in a symbolic linear depiction known as a sequence which succinctly summarizes much of the atomic-level structure of the sequenced molecule. DNA sequencing DNA sequencing is the process of determining the nucleotide order of a given DNA fragment. So far, most DNA sequencing has been performed using the chain termination method developed by Frederick Sanger.
Shotgun sequencingIn genetics, shotgun sequencing is a method used for sequencing random DNA strands. It is named by analogy with the rapidly expanding, quasi-random shot grouping of a shotgun. The chain-termination method of DNA sequencing ("Sanger sequencing") can only be used for short DNA strands of 100 to 1000 base pairs. Due to this size limit, longer sequences are subdivided into smaller fragments that can be sequenced separately, and these sequences are assembled to give the overall sequence.
Sanger sequencingSanger sequencing is a method of DNA sequencing that involves electrophoresis and is based on the random incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication. After first being developed by Frederick Sanger and colleagues in 1977, it became the most widely used sequencing method for approximately 40 years. It was first commercialized by Applied Biosystems in 1986. More recently, higher volume Sanger sequencing has been replaced by next generation sequencing methods, especially for large-scale, automated genome analyses.
Whole genome sequencingWhole genome sequencing (WGS), also known as full genome sequencing, complete genome sequencing, or entire genome sequencing, is the process of determining the entirety, or nearly the entirety, of the DNA sequence of an organism's genome at a single time. This entails sequencing all of an organism's chromosomal DNA as well as DNA contained in the mitochondria and, for plants, in the chloroplast. Whole genome sequencing has largely been used as a research tool, but was being introduced to clinics in 2014.
Exome sequencingExome sequencing, also known as whole exome sequencing (WES), is a genomic technique for sequencing all of the protein-coding regions of genes in a genome (known as the exome). It consists of two steps: the first step is to select only the subset of DNA that encodes proteins. These regions are known as exons—humans have about 180,000 exons, constituting about 1% of the human genome, or approximately 30 million base pairs. The second step is to sequence the exonic DNA using any high-throughput DNA sequencing technology.
Massive parallel sequencingMassive parallel sequencing or massively parallel sequencing is any of several high-throughput approaches to DNA sequencing using the concept of massively parallel processing; it is also called next-generation sequencing (NGS) or second-generation sequencing. Some of these technologies emerged between 1993 and 1998 and have been commercially available since 2005. These technologies use miniaturized and parallelized platforms for sequencing of 1 million to 43 billion short reads (50 to 400 bases each) per instrument run.
LandscapeA landscape is the visible features of an area of land, its landforms, and how they integrate with natural or man-made features, often considered in terms of their aesthetic appeal. A landscape includes the physical elements of geophysically defined landforms such as (ice-capped) mountains, hills, water bodies such as rivers, lakes, ponds and the sea, living elements of land cover including indigenous vegetation, human elements including different forms of land use, buildings, and structures, and transitory elements such as lighting and weather conditions.
Cultural landscapeCultural landscape is a term used in the fields of geography, ecology, and heritage studies, to describe a symbiosis of human activity and environment. As defined by the World Heritage Committee, it is the "cultural properties [that] represent the combined works of nature and of man" and falls into three main categories: "a landscape designed and created intentionally by man" an "organically evolved landscape" which may be a "relict (or fossil) landscape" or a "continuing landscape" an "associative cultural landscape" which may be valued because of the "religious, artistic or cultural associations of the natural element.
Physical geographyPhysical geography (also known as physiography) is one of the three main branches of geography. Physical geography is the branch of natural science which deals with the processes and patterns in the natural environment such as the atmosphere, hydrosphere, biosphere, and geosphere. This focus is in contrast with the branch of human geography, which focuses on the built environment, and technical geography, which focuses on using, studying, and creating tools to obtain,analyze, interpret, and understand spatial information.
GeographyGeography (from Greek: γεωγραφία, geographia. Combination of Greek words 'Geo' (The Earth) and 'Graphien' (to describe), literally "earth description") is a field of science devoted to the study of the lands, features, inhabitants, and phenomena of Earth. Geography is an all-encompassing discipline that seeks an understanding of Earth and its human and natural complexities—not merely where objects are, but also how they have changed and come to be.
Projective moduleIn mathematics, particularly in algebra, the class of projective modules enlarges the class of free modules (that is, modules with basis vectors) over a ring, by keeping some of the main properties of free modules. Various equivalent characterizations of these modules appear below. Every free module is a projective module, but the converse fails to hold over some rings, such as Dedekind rings that are not principal ideal domains.
EconomyAn economy is an area of the production, distribution and trade, as well as consumption of goods and services. In general, it is defined as a social domain that emphasize the practices, discourses, and material expressions associated with the production, use, and management of scarce resources. A given economy is a set of processes that involves its culture, values, education, technological evolution, history, social organization, political structure, legal systems, and natural resources as main factors.
Landscape ecologyLandscape ecology is the science of studying and improving relationships between ecological processes in the environment and particular ecosystems. This is done within a variety of landscape scales, development spatial patterns, and organizational levels of research and policy. Concisely, landscape ecology can be described as the science of "landscape diversity" as the synergetic result of biodiversity and geodiversity.
CultureCulture (ˈkʌltʃər) is an umbrella term which encompasses the social behavior, institutions, and norms found in human societies, as well as the knowledge, beliefs, arts, laws, customs, capabilities, and habits of the individuals in these groups. Culture is often originated from or attributed to a specific region or location. Humans acquire culture through the learning processes of enculturation and socialization, which is shown by the diversity of cultures across societies.
Massive open online courseA massive open online course (MOOC muːk) or an open online course is an online course aimed at unlimited participation and open access via the Web. In addition to traditional course materials, such as filmed lectures, readings, and problem sets, many MOOCs provide interactive courses with user forums or social media discussions to support community interactions among students, professors, and teaching assistants (TAs), as well as immediate feedback to quick quizzes and assignments.
Module (mathematics)In mathematics, a module is a generalization of the notion of vector space in which the field of scalars is replaced by a ring. The concept of module generalizes also the notion of abelian group, since the abelian groups are exactly the modules over the ring of integers. Like a vector space, a module is an additive abelian group, and scalar multiplication is distributive over the operation of addition between elements of the ring or module and is compatible with the ring multiplication.
SocietyA society ( səˈsaɪəti) is a group of individuals involved in persistent social interaction or a large social group sharing the same spatial or social territory, typically subject to the same political authority and dominant cultural expectations. Societies are characterized by patterns of relationships (social relations) between individuals who share a distinctive culture and institutions; a given society may be described as the sum total of such relationships among its constituent members.
Free moduleIn mathematics, a free module is a module that has a basis, that is, a generating set consisting of linearly independent elements. Every vector space is a free module, but, if the ring of the coefficients is not a division ring (not a field in the commutative case), then there exist non-free modules. Given any set S and ring R, there is a free R-module with basis S, which is called the free module on S or module of formal R-linear combinations of the elements of S. A free abelian group is precisely a free module over the ring Z of integers.
Landscape archaeologyLandscape archaeology, a sub-discipline of archaeology and archaeological theory, is the study of the ways in which people in the past constructed and used the environment around them. It is also known as archaeogeography (from the Greek ἀρχαίος "ancient", and γεωγραφία "earth study"). Landscape archaeology is inherently multidisciplinary in its approach to the study of culture, and is used by pre-historical, classic, and historic archaeologists.
