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This lecture explores the mechanisms of speciation, focusing on the barriers that prevent gene flow between populations. It covers pre-zygotic and post-zygotic barriers such as ecological, temporal, ethological, mechanical, and gametic isolation. Examples are provided to illustrate these concepts, emphasizing the importance of understanding the nature of each barrier. The lecture also discusses hybrid zones and the outcomes of hybridization, including reinforcement, fusion, and stability. It concludes with a summary of speciation, highlighting the role of reproductive isolation in the formation of new species.