Lecture

RNA-Protein Interaction: Experimental Analysis

Graph Chat

Chat with Graph Search

Ask any question about EPFL courses, lectures, exercises, research, news, etc. or try the example questions below.

DISCLAIMER: The Graph Chatbot is not programmed to provide explicit or categorical answers to your questions. Rather, it transforms your questions into API requests that are distributed across the various IT services officially administered by EPFL. Its purpose is solely to collect and recommend relevant references to content that you can explore to help you answer your questions.

Description

This lecture covers the experimental analysis of RNA-protein interactions, focusing on identifying novel human proteins that target a specific viral RNA. Topics include the use of small-molecule tools, in-gel fluorescence analysis, and the design of experimental workflows. The lecture also discusses the application of genetic code expansion to track proteolytic processing and trafficking of amyloid beta peptide in Alzheimer's disease.

This video is available exclusively on Mediaspace for a restricted audience. Please log in to MediaSpace to access it if you have the necessary permissions.

Watch on Mediaspace

Official source

Related concepts (32)

Virus

A virus is a submicroscopic infectious agent that replicates only inside the living cells of an organism. Viruses infect all life forms, from animals and plants to microorganisms, including bacteria and archaea. Since Dmitri Ivanovsky's 1892 article describing a non-bacterial pathogen infecting tobacco plants and the discovery of the tobacco mosaic virus by Martinus Beijerinck in 1898, more than 11,000 of the millions of virus species have been described in detail.

Respiratory syncytial virus

Respiratory syncytial virus (RSV), also called human respiratory syncytial virus (hRSV) and human orthopneumovirus, is a common, contagious virus that causes infections of the respiratory tract. It is a negative-sense, single-stranded RNA virus. Its name is derived from the large cells known as syncytia that form when infected cells fuse. RSV is a common cause of respiratory hospitalization in infants, and reinfection remains common in later life: it is a notable pathogen in all age groups.

Viral load

Viral load, also known as viral burden, is a numerical expression of the quantity of virus in a given volume of fluid, including biological and environmental specimens. It is not to be confused with viral titre or viral titer, which depends on the assay. When an assay for measuring the infective virus particle is done (Plaque assay, Focus assay), viral titre often refers to the concentration of infectious viral particles, which is different from the total viral particles. Viral load is measured using body fluids Sputum and blood plasma.

RNA editing

RNA editing (also RNA modification) is a molecular process through which some cells can make discrete changes to specific nucleotide sequences within an RNA molecule after it has been generated by RNA polymerase. It occurs in all living organisms and is one of the most evolutionarily conserved properties of RNAs. RNA editing may include the insertion, deletion, and base substitution of nucleotides within the RNA molecule. RNA editing is relatively rare, with common forms of RNA processing (e.g.

Amyloid beta

Amyloid beta (Aβ or Abeta) denotes peptides of 36–43 amino acids that are the main component of the amyloid plaques found in the brains of people with Alzheimer's disease. The peptides derive from the amyloid-beta precursor protein (APP), which is cleaved by beta secretase and gamma secretase to yield Aβ in a cholesterol-dependent process and substrate presentation. Aβ molecules can aggregate to form flexible soluble oligomers which may exist in several forms.

Related lectures (61)

Protein Aggregation in Neurodegenerative Diseases

Explores protein aggregation in neurodegenerative diseases, emphasizing the need to rethink treatment strategies and understand the role of post-translational modifications.

Parkinson's Disease Alpha-Synuclein: Amyloid Formation and Cross-reactivity

Explores Parkinson's alpha-synuclein cross-reactivity, amyloid formation, compound impact, and copper chaperones.

Explores SERF-related proteins' functions in amyloid acceleration and G-quadruplex binding, shedding light on their complex mechanisms.

Protein Synthesis: Recombinant Expression

Explores protein synthesis, genetic code, transcription, translation, and recombinant protein expression using vectors and plasmids.

Protein Production: Engineering Bacteria for Protein Synthesis