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This lecture covers special topics in indoor air quality and ventilation, focusing on recent studies using culture-independent techniques to understand the microbial ecology of the built environment. It discusses case studies on indoor bacteria sources, human microbial emissions, and the transmission modes of respiratory pathogens like SARS-CoV-2. The lecture also explores the impact of respiratory activities on aerosol transmission, emphasizing the importance of particle size in virus transmission. Additionally, it delves into the efficiency of masks in blocking aerosols of different sizes from reaching the lower lungs. Various visualization techniques and research findings are presented to illustrate these concepts.