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Lecture

Biophotonics Fundamentals

In course

BIO-443: Fundamentals of biophotonics

This module serves as an introduction to the area of biophotonics. The approach is multidisciplinary .The course is mainly knowledge-based but students will benefit from the skills learned by carrying out problem solving and by completing the assignment. ...

Description

This lecture covers the fundamentals of biophotonics, focusing on the Franck-Condon rule, absorption/transmittance, Beer-Lambert Law, fluorescence, phosphorescence, and the principles of spectroscopy. It explains the influence of energy level populations, temperature, and energy level spacing on absorption. The Franck-Condon principle is discussed in relation to electronic transitions and vibrational states. The lecture also delves into the quantum yield of fluorescence, fluorescence intensity, and the role of chromophores and fluorophores in biological systems. Additionally, it explores the absorption and emission spectra of biological chromophores, nucleic acids, and lipids, as well as the use of NADH and FAD as metabolic indicators.

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Instructor

Aleksandra Radenovic

From April 2021 Full Professor 2015 -2021 Associate Professor2008-2015 Tenure-Track Assistant Professor2004-2007 Postdoc at the University of California, Berkeley in the group of Prof.Liphardt2003 PhD student of Prof. Dietler in Laboratory of Physics of Living Matter, University of Lausanne 1999 Diploma thesis on the subject of the Raman spectroscopy of beta carotene1994-1999 Physics department at the University of Zagreb1994 baccalaureate, Classical gymnasium

Official source

Ontological neighbourhood

Chemistry

Physical chemistry: Spectroscopy

Related concepts (32)

Absorption spectroscopy

Absorption spectroscopy refers to spectroscopic techniques that measure the absorption of electromagnetic radiation, as a function of frequency or wavelength, due to its interaction with a sample. The sample absorbs energy, i.e., photons, from the radiating field. The intensity of the absorption varies as a function of frequency, and this variation is the absorption spectrum. Absorption spectroscopy is performed across the electromagnetic spectrum.

Absorbance

Absorbance is defined as "the logarithm of the ratio of incident to transmitted radiant power through a sample (excluding the effects on cell walls)". Alternatively, for samples which scatter light, absorbance may be defined as "the negative logarithm of one minus absorptance, as measured on a uniform sample". The term is used in many technical areas to quantify the results of an experimental measurement. While the term has its origin in quantifying the absorption of light, it is often entangled with quantification of light which is “lost” to a detector system through other mechanisms.

Spectroscopy

Spectroscopy is the field of study that measures and interprets the electromagnetic spectra that result from the interaction between electromagnetic radiation and matter as a function of the wavelength or frequency of the radiation. Matter waves and acoustic waves can also be considered forms of radiative energy, and recently gravitational waves have been associated with a spectral signature in the context of the Laser Interferometer Gravitational-Wave Observatory (LIGO).

Transmittance

Transmittance of the surface of a material is its effectiveness in transmitting radiant energy. It is the fraction of incident electromagnetic power that is transmitted through a sample, in contrast to the transmission coefficient, which is the ratio of the transmitted to incident electric field. Internal transmittance refers to energy loss by absorption, whereas (total) transmittance is that due to absorption, scattering, reflection, etc.

Emission spectrum

The emission spectrum of a chemical element or chemical compound is the spectrum of frequencies of electromagnetic radiation emitted due to an electron making a transition from a high energy state to a lower energy state. The photon energy of the emitted photon is equal to the energy difference between the two states. There are many possible electron transitions for each atom, and each transition has a specific energy difference. This collection of different transitions, leading to different radiated wavelengths, make up an emission spectrum.

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