Lecture

Single Molecule FRET

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In course

BIO-443: Fundamentals of biophotonics

This module serves as an introduction to the area of biophotonics. The approach is multidisciplinary .The course is mainly knowledge-based but students will benefit from the skills learned by carrying out problem solving and by completing the assignment. ...

Description

This lecture covers the fundamentals of Biophotonics, focusing on Single Molecule FRET (Förster Resonance Energy Transfer) with Total Internal Reflection Microscopy. It explains the non-radiative energy transfer between molecules, the conditions required for energy transfer, and the selection of appropriate fluorophores. The lecture also discusses the applications of FRET in studying protein structure, protein complexes, receptor interactions, and nucleic acids. It delves into the labeling of molecules for FRET experiments, the advantages of single molecule FRET, and the techniques for surface immobilization. Additionally, it explores the principles of Total Internal Reflection Fluorescence (TIRF) microscopy and its applications in imaging cells. The lecture concludes with a discussion on TIRF instrumentation configurations and practical examples of TIRF microscopy.

Instructor

Aleksandra Radenovic

From April 2021 Full Professor 2015 -2021 Associate Professor2008-2015 Tenure-Track Assistant Professor2004-2007 Postdoc at the University of California, Berkeley in the group of Prof.Liphardt2003 PhD student of Prof. Dietler in Laboratory of Physics of Living Matter, University of Lausanne 1999 Diploma thesis on the subject of the Raman spectroscopy of beta carotene1994-1999 Physics department at the University of Zagreb1994 baccalaureate, Classical gymnasium

Official source

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