Tandem mass spectrometryTandem mass spectrometry, also known as MS/MS or MS2, is a technique in instrumental analysis where two or more mass analyzers are coupled together using an additional reaction step to increase their abilities to analyse chemical samples. A common use of tandem MS is the analysis of biomolecules, such as proteins and peptides. The molecules of a given sample are ionized and the first spectrometer (designated MS1) separates these ions by their mass-to-charge ratio (often given as m/z or m/Q).
18-electron ruleThe 18-electron rule is a chemical rule of thumb used primarily for predicting and rationalizing formulas for stable transition metal complexes, especially organometallic compounds. The rule is based on the fact that the valence orbitals in the electron configuration of transition metals consist of five (n−1)d orbitals, one ns orbital, and three np orbitals, where n is the principal quantum number. These orbitals can collectively accommodate 18 electrons as either bonding or non-bonding electron pairs.
Natural abundanceIn physics, natural abundance (NA) refers to the abundance of isotopes of a chemical element as naturally found on a planet. The relative atomic mass (a weighted average, weighted by mole-fraction abundance figures) of these isotopes is the atomic weight listed for the element in the periodic table. The abundance of an isotope varies from planet to planet, and even from place to place on the Earth, but remains relatively constant in time (on a short-term scale). As an example, uranium has three naturally occurring isotopes: 238U, 235U, and 234U.
Relative species abundanceRelative species abundance is a component of biodiversity and is a measure of how common or rare a species is relative to other species in a defined location or community. Relative abundance is the percent composition of an organism of a particular kind relative to the total number of organisms in the area. Relative species abundances tend to conform to specific patterns that are among the best-known and most-studied patterns in macroecology. Different populations in a community exist in relative proportions; this idea is known as relative abundance.
Abundance (ecology)In ecology, local abundance is the relative representation of a species in a particular ecosystem. It is usually measured as the number of individuals found per sample. The ratio of abundance of one species to one or multiple other species living in an ecosystem is referred to as relative species abundances. Both indicators are relevant for computing biodiversity. A variety of sampling methods are used to measure abundance. For larger animals, these may include spotlight counts, track counts and roadkill counts, as well as presence at monitoring stations.
Unix shellA Unix shell is a command-line interpreter or shell that provides a command line user interface for Unix-like operating systems. The shell is both an interactive command language and a scripting language, and is used by the operating system to control the execution of the system using shell scripts. Users typically interact with a Unix shell using a terminal emulator; however, direct operation via serial hardware connections or Secure Shell are common for server systems.
Secure ShellThe Secure Shell Protocol (SSH) is a cryptographic network protocol for operating network services securely over an unsecured network. Its most notable applications are remote login and command-line execution. SSH applications are based on a client–server architecture, connecting an SSH client instance with an SSH server. SSH operates as a layered protocol suite comprising three principal hierarchical components: the transport layer provides server authentication, confidentiality, and integrity; the user authentication protocol validates the user to the server; and the connection protocol multiplexes the encrypted tunnel into multiple logical communication channels.
OpenSSHOpenSSH (also known as OpenBSD Secure Shell) is a suite of secure networking utilities based on the Secure Shell (SSH) protocol, which provides a secure channel over an unsecured network in a client–server architecture. OpenSSH started as a fork of the free SSH program developed by Tatu Ylönen; later versions of Ylönen's SSH were proprietary software offered by SSH Communications Security. OpenSSH was first released in 1999 and is currently developed as part of the OpenBSD operating system.
Abundance of the chemical elementsThe abundance of the chemical elements is a measure of the occurrence of the chemical elements relative to all other elements in a given environment. Abundance is measured in one of three ways: by mass fraction (in commercial contexts often called weight fraction), by mole fraction (fraction of atoms by numerical count, or sometimes fraction of molecules in gases), or by volume fraction. Volume fraction is a common abundance measure in mixed gases such as planetary atmospheres, and is similar in value to molecular mole fraction for gas mixtures at relatively low densities and pressures, and ideal gas mixtures.
Mass spectral interpretationMass spectral interpretation is the method employed to identify the chemical formula, characteristic fragment patterns and possible fragment ions from the mass spectra. Mass spectra is a plot of relative abundance against mass-to-charge ratio. It is commonly used for the identification of organic compounds from electron ionization mass spectrometry. Organic chemists obtain mass spectra of chemical compounds as part of structure elucidation and the analysis is part of many organic chemistry curricula.
Reference materials for stable isotope analysisIsotopic reference materials are compounds (solids, liquids, gasses) with well-defined isotopic compositions and are the ultimate sources of accuracy in mass spectrometric measurements of isotope ratios. Isotopic references are used because mass spectrometers are highly fractionating. As a result, the isotopic ratio that the instrument measures can be very different from that in the sample's measurement. Moreover, the degree of instrument fractionation changes during measurement, often on a timescale shorter than the measurement's duration, and can depend on the characteristics of the sample itself.
Diatomic moleculeDiatomic molecules () are molecules composed of only two atoms, of the same or different chemical elements. If a diatomic molecule consists of two atoms of the same element, such as hydrogen () or oxygen (), then it is said to be homonuclear. Otherwise, if a diatomic molecule consists of two different atoms, such as carbon monoxide () or nitric oxide (), the molecule is said to be heteronuclear. The bond in a homonuclear diatomic molecule is non-polar.
