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Lecture

Transmission Electron Microscopy: Weak Phase Object Approximation

In course

MSE-450: Electron microscopy: advanced methods

With this course, the student will learn about advanced methods in transmission electron microscopy, especially what is the electron optical setup involved in the acquisition, and how to interpret the data. After the course, students will be able to unders ...

Description

This lecture covers the principles of Transmission Electron Microscopy, focusing on the Weak Phase Object Approximation. It explains how a thin specimen affects the phase, the concept of the Weak Phase Object Approximation, and the Phase Contrast Transfer Function. The instructor concludes by summarizing the key points.

In MOOC

Transmission Electron Microscopy for Materials Sciences

Instructors (2)

Cécile Hébert

Cécile Hébert was born in France in 1970. She obtained her Ingeneer degree (physics) and her PhD degree (design of a new energy filter for transmission electron microscopy, under the direction of Prof. B. Jouffrey) at the Ecole Centrale in Paris. As a post doc with Prof. P. Schattschneider, she was working on the calculation of the fine structure of ionisation edges in EELS. As a research assistant at the Vienna University of Technology, she was one of the main participant to the CHIRALTEM project dealing with the measurement of magnetic circular dichroism in the electron microscope.

Duncan Thomas Lindsay Alexander

Duncan Alexander graduated with a PhD in Materials Science and Metallurgy at the University of Cambridge in 2003 for a thesis on the kinetics of metallurgical phase transformations under the direction of Lindsay Greer. From 2003 to 2007 he held a Royal Society Overseas Research Fellowship at the University of Sydney, and then post-doctoral positions at the University of Cambridge and Arizona State University, working first on materials electrochemistry with Carsten Schwandt and Derek Fray, and then the characterization of atmospheric aerosol particles with Peter Crozier and Jim Anderson. In 2007 he came to EPFL as a scientific collaborator in the group of László Forró, and from 2008 to 2017 he was a staff scientist at EPFL’s Interdisciplinary Centre for Electron Microscopy (CIME), specializing in the application of advanced transmission electron microscopy techniques and enabling electron microscopy research activities for groups across EPFL through teaching, training and support. In 2018 he joined the Electron Spectrometry and Microscopy Laboratory (LSME) as a full-time research scientist dedicated to advancing transmission electron microscopy techniques, and was promoted to the status of Research and Teaching Associate. In 2019, together with LSME director Cécile Hébert, he launched the first MOOC on transmission electron microscopy for materials science: https://www.coursera.org/learn/microscopy/For a complete list of peer-reviewed publications, see:https://orcid.org/0000-0003-4350-8587

Official source

Related concepts (14)

Transmission electron cryomicroscopy

Transmission electron cryomicroscopy (CryoTEM), commonly known as cryo-EM, is a form of cryogenic electron microscopy, more specifically a type of transmission electron microscopy (TEM) where the sample is studied at cryogenic temperatures (generally liquid-nitrogen temperatures). Cryo-EM is gaining popularity in structural biology. The utility of transmission electron cryomicroscopy stems from the fact that it allows the observation of specimens that have not been stained or fixed in any way, showing them in their native environment.

Scanning transmission electron microscopy

A scanning transmission electron microscope (STEM) is a type of transmission electron microscope (TEM). Pronunciation is [stɛm] or [ɛsti:i:ɛm]. As with a conventional transmission electron microscope (CTEM), images are formed by electrons passing through a sufficiently thin specimen. However, unlike CTEM, in STEM the electron beam is focused to a fine spot (with the typical spot size 0.05 – 0.2 nm) which is then scanned over the sample in a raster illumination system constructed so that the sample is illuminated at each point with the beam parallel to the optical axis.

Transmission electron microscopy

Transmission electron microscopy (TEM) is a microscopy technique in which a beam of electrons is transmitted through a specimen to form an image. The specimen is most often an ultrathin section less than 100 nm thick or a suspension on a grid. An image is formed from the interaction of the electrons with the sample as the beam is transmitted through the specimen. The image is then magnified and focused onto an imaging device, such as a fluorescent screen, a layer of photographic film, or a sensor such as a scintillator attached to a charge-coupled device.

Electron microscope

An electron microscope is a microscope that uses a beam of electrons as a source of illumination. They use electron optics that are analogous to the glass lenses of an optical light microscope. As the wavelength of an electron can be up to 100,000 times shorter than that of visible light, electron microscopes have a higher resolution of about 0.1 nm, which compares to about 200 nm for light microscopes.

Cryogenic electron microscopy

Cryogenic electron microscopy (cryo-EM) is a cryomicroscopy technique applied on samples cooled to cryogenic temperatures. For biological specimens, the structure is preserved by embedding in an environment of vitreous ice. An aqueous sample solution is applied to a grid-mesh and plunge-frozen in liquid ethane or a mixture of liquid ethane and propane. While development of the technique began in the 1970s, recent advances in detector technology and software algorithms have allowed for the determination of biomolecular structures at near-atomic resolution.

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