Lecture

DNA Replication: Eukaryotes

Graph Chat

Chat with Graph Search

Ask any question about EPFL courses, lectures, exercises, research, news, etc. or try the example questions below.

DISCLAIMER: The Graph Chatbot is not programmed to provide explicit or categorical answers to your questions. Rather, it transforms your questions into API requests that are distributed across the various IT services officially administered by EPFL. Its purpose is solely to collect and recommend relevant references to content that you can explore to help you answer your questions.

In course

BIOENG-110: General Biology

Le but du cours est de fournir un aperçu général de la biologie des cellules et des organismes. Nous en discuterons dans le contexte de la vie des cellules et des organismes, en mettant l'accent sur les principes de réglementation que vous rencontrerez dan ...

Description

This lecture covers the process of DNA replication in eukaryotic cells, focusing on the formation of pre-replicative complexes at replication origins, the activation and inactivation of key proteins such as Cdk and APC/C, and the mechanisms that limit DNA replication to once per cell cycle. The lecture also discusses the role of licensing factors, the assembly of replication initiation complexes, and the regulation of replication through phosphorylation and proteolysis.

Instructors (5)

Freddy Radtke

Freddy Radtke obtained his Ph.D. in Molecular Biology from the University of Zürich in 1994. In 1995, he started his postdoctoral research in the laboratory of Michel Aguet at Genentech, Inc. (San Francisco, USA). In 1997, he returned to Switzerland with Michel Aguet and finished his postdoctoral fellowship at the Swiss Institute for Experimental Cancer Research (ISREC) in Lausanne. From 1999‑2005, he was a group leader and Associate Member at the Ludwig Institute for Cancer Research. Freddy Radtke then joined ISREC in January 2006 as a senior scientist and in July 2006, he was appointed Associate Professor at the EPFL School of Life Sciences

Viesturs Simanis

Viesturs Simanis was awarded a degree in Biochemistry from Imperial College London. He carried out his doctoral studies with David Lane at Imperial College, London University, and postdoctoral studies with Paul Nurse (London and Oxford). He has been a group leader at ISREC since 1988. In 2006 he was appointed Associate Professor at the EPFL School of Life Sciences

Kristina Schoonjans

Expertise Bile acid signalingNutrient sensingIntermediary metabolismMetabolic disorders Teaching & PhD PhD Students Adrien Faure Past EPFL PhD Students Pan Xu, Ece Yildiz, Nadia Bresciani, Yu Sun, Francesca Pontanari, Alejandro Alonso Calleja Past EPFL PhD Students as codirector Mitsunori Nomura, Pan Xu, Katyayanee Neopane, Lucie Camille Chanvillard, Jakob Josef Langer, Bilge Sen Elçi Courses General Biology BIOENG-110 The purpose of the course is to provide a general overview of the biolo

Johannes Gräff

Academic appointments 2013- Tenure Track Assistant Professor, Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland, School of Life Sciences, Brain Mind Institute. 2009-2013 Postdoctoral fellow, Massachusetts Institute of Technology (MIT), Cambridge, USA, Picower Institute for Learning and Memory. 2009 Postdoctoral associate, Swiss Federal Institute of Technology (ETHZ), Zürich, Switzerland, Brain Research Institute. Education 2009 PhD in Neurosciences, Swiss Federal Institute of Technology (ETHZ), Zürich, Switzerland, Department of Biology, Brain Research Institute. 2005 Diploma (M.Sc. equivalent) in Biological Sciences, University of Lausanne, Switzerland, Faculty of Biology and Medicine, Department of Ecology and Evolution. 1998 Matura, Gymnasium Type B, Kantonsschule am Burggraben St. Gallen, Switzerland.

Official source

Ontological neighbourhood

Biology

Cell biology: Cell cycle

Related concepts (32)

Origin of replication

The origin of replication (also called the replication origin) is a particular sequence in a genome at which replication is initiated. Propagation of the genetic material between generations requires timely and accurate duplication of DNA by semiconservative replication prior to cell division to ensure each daughter cell receives the full complement of chromosomes. This can either involve the replication of DNA in living organisms such as prokaryotes and eukaryotes, or that of DNA or RNA in viruses, such as double-stranded RNA viruses.

Cell cycle checkpoint

Cell cycle checkpoints are control mechanisms in the eukaryotic cell cycle which ensure its proper progression. Each checkpoint serves as a potential termination point along the cell cycle, during which the conditions of the cell are assessed, with progression through the various phases of the cell cycle occurring only when favorable conditions are met. There are many checkpoints in the cell cycle, but the three major ones are: the G1 checkpoint, also known as the Start or restriction checkpoint or Major Checkpoint; the G2/M checkpoint; and the metaphase-to-anaphase transition, also known as the spindle checkpoint.

Phosphorylation

In biochemistry, phosphorylation is the attachment of a phosphate group to a molecule or an ion. This process and its inverse, dephosphorylation, are common in biology. Protein phosphorylation often activates (or deactivates) many enzymes. Phosphorylation is essential to the processes of both anaerobic and aerobic respiration, which involve the production of adenosine triphosphate (ATP), the "high-energy" exchange medium in the cell.

Cohesin

Cohesin is a protein complex that mediates sister chromatid cohesion, homologous recombination, and DNA looping. Cohesin is formed of SMC3, SMC1, SCC1 and SCC3 (SA1 or SA2 in humans). Cohesin holds sister chromatids together after DNA replication until anaphase when removal of cohesin leads to separation of sister chromatids. The complex forms a ring-like structure and it is believed that sister chromatids are held together by entrapment inside the cohesin ring.

Oxidative phosphorylation

Oxidative phosphorylation (UK ɒkˈsɪd.ə.tɪv, US ˈɑːk.sɪˌdeɪ.tɪv ) or electron transport-linked phosphorylation or terminal oxidation is the metabolic pathway in which cells use enzymes to oxidize nutrients, thereby releasing chemical energy in order to produce adenosine triphosphate (ATP). In eukaryotes, this takes place inside mitochondria. Almost all aerobic organisms carry out oxidative phosphorylation. This pathway is so pervasive because it releases more energy than alternative fermentation processes such as anaerobic glycolysis.

Related lectures (59)

DNA Replication: Sister Chromatids Cohesion

Explores DNA replication in eukaryotes and the cohesion of sister chromatids.

Cellular Biology: Mitosis and Meiosis Overview

Covers cellular biology, focusing on mitosis, meiosis, and the cell cycle processes.

DNA Replication: Mechanisms and Errors

Explores DNA replication mechanisms, including speed, errors, proofreading, and semiconservative nature, emphasizing the importance of maintaining DNA fidelity through mismatch repair.

DNA Structure and Function

Explores DNA structure, histone interactions, polymerase function, nucleosome organization, chromatin compaction, cell cycle phases, FISH analysis, and telomere structure.

DNA Structure and Replication