Lectures in course MICRO-560: BioMEMS

SensUs presentation Log in to Mediaspace to watch this video

Introduces the SensUs competition, where students design and test functional sensors.

SensUs Presentation: Organized Work and Technical Approach Log in to Mediaspace to watch this video

Explains the organization of the SensUs project and the use of Molecularly Imprinted Polymers in sensor technology.

DNA separation: Methods and Techniques Log in to Mediaspace to watch this video

Explores DNA separation methods, including gel and capillary electrophoresis, nanochannel sizing, and protein binding site observation.

DNA amplification Log in to Mediaspace to watch this video

Covers the principles and applications of DNA amplification techniques, focusing on Polymerase Chain Reaction (PCR) and digital PCR.

DNA Sequencing Methods Log in to Mediaspace to watch this video

Explores DNA sequencing methods like Sanger, pyrosequencing, and synthesis technologies.

Single Molecule Sequencing: Strategies and Technologies Log in to Mediaspace to watch this video

Explores single molecule sequencing strategies, including sequencing by synthesis and real-time sequencing based on zero mode waveguides, as well as DNA translocation through nanopores.

Single Cell mRNA Sequencing Log in to Mediaspace to watch this video

Explores the Drop Seq method for single-cell mRNA sequencing and compares it with RNA Microarray.

DNA & Protein Arrays Log in to Mediaspace to watch this video

Explores DNA array applications, fabrication methods, SNP detection, protein array usage, array patterning, and microarray vs. RNA-Seq comparison.

Protein Separation Log in to Mediaspace to watch this video

Explores protein separation techniques, including isoelectric focusing and capillary electrophoresis, for protein identification and profiling.

Immunoassays: Basics and Techniques Log in to Mediaspace to watch this video

Covers the basics and techniques of immunoassays, including reaction kinetics, ELISA, chemiluminescence, and multiplex assays.

Immunoassays: Kinetics and Limitations Log in to Mediaspace to watch this video

Explores the kinetics and limitations of immunoassays, including equilibrium surface capture and microchannel techniques.

Paper: Capillary Flow Progression Log in to Mediaspace to watch this video

Explores capillary flow progression in paper microfluidics, lateral flow assays, gold nanoparticles in bioassays, automated LFA readers, and test results evaluation.

Introduction: BioMEMS Overview Log in to Mediaspace to watch this video

Introduces BioMEMS, covering applications like DNA separation, protein analysis, and neuroelectronic implants, along with key numbers in biology and challenges in system integration.

Surface tension: Capillary Effects Log in to Mediaspace to watch this video

Explores surface tension effects in microfluidic channels and capillary stop valves.

Droplets: Formation and Manipulation Log in to Mediaspace to watch this video

Explores the formation, manipulation, and encapsulation of droplets in microfluidic systems, highlighting the role of surfactants and different techniques for droplet control.

Surfaces Functionalization Log in to Mediaspace to watch this video

Explores the modification of surfaces to change properties and attach new molecules.

Cell Adhesion Patterning Log in to Mediaspace to watch this video

Explores creating cell adhesion patterns using various molecules and techniques, including ECM molecules, RGD coatings, and PEG for cell repulsion.

Cell Traps: Microfluidic Devices and Arrays Log in to Mediaspace to watch this video

Explores microfluidic cell trapping, array technologies, and immunocapture methods for single cells, highlighting the importance of studying individual cell behavior.

Electrochemical Sensing Log in to Mediaspace to watch this video

Explores electrochemical biosensors, amperometry, glucose sensors, enzymatic biosensors, mediators, cyclic voltammetry, and electrochemiluminescence in biosensors.

Potentiometry: Fundamentals and Applications Log in to Mediaspace to watch this video

Explores potentiometry, ISFET technology, DNA detection, ion-selective electrodes, and wearable devices for biological fluid monitoring.