David HackerExpertise
Animal cell cultivationTransient transfectionStable cell line generationExpression vector designProtein purification
Teaching & PhD Past EPFL PhD Students as codirector Agata Oberbek, Yashas Rajendra, Xiao Shen, Dominique Terry Monteil Jr, Sowmya Balasubramanian Courses Recombinant protein expression in animal cells for appli-cations in medicine and structural biology BIO-701 Cultivated animal cells are important hosts for the production of recombinant proteins for biochemical and
Jérôme Paul Rémy ThevenotExpertise
Biomechanics and biomedical engineeringMedical devices and smart wearablesSignal and medical images processingPrototyping, 3D modeling and finite element analysis3D printing, silicone moldingClinical trials and animal experimentation
Expertise
Biomechanics and biomedical engineeringMedical devices and smart wearablesSignal and medical images processingPrototyping, 3D modeling and finite element analysisClinical trials and animal experimentation
Education PhD Biomedicine | Thesis: Biomechanica
Kelvin Ka Ching LauExpertise
Protein expressionProtein purificationProtein characterizationProtein-protein interactionsX-ray crystallographyStructural biologyProtein biophysics
Mission
I am trained in biochemistry and structural biology and have keen interest in protein-protein interactions. Here to help solve your problems!
Education PhD | Biochemistry and Molecular Biology 2008 – 2014 University of British Columbia Directed by Filip van Petegem Bachelor of Science | Honours Bi
Yoan Désiré DuhooTeaching & PhD Courses Integrative structural biology for Life sciences BIO-643 Hands-on course in Biomolecular Integrative Structural Biology by SV experts in the field of X-ray crystallography, cryo-Electron Microscopy, Bio-NMR and protein modeling tools. No previous knowledge in Structural Biology or Bioinformatics is required. Recombinant protein expression in animal cells for appli-cations in medicine and structural biology BIO-701 Cultivated animal cells are important
Florence PojerExpertise
MER and Head of Unit - Protein Production and Structure Reseach Core Facility within the Life Sciences School - PTPSP: Expertise in Integrative protein Structural Biology (mainly X-ray Crystallography and Cryo-EM) and production of macromolecules in mammalian and bacterial cells. Innovate4Life team member at SV: i4L supports the next generation of entrepreneurs through education, mentorship, and funding, to translate life sciences discoveries into technologies that benefit society. Education and
Luciano Andres AbriataExpertise
Protein NMRNMR of paramagnetic systemsMetalloproteinsBiomolecular SpectroscopyStructural BiologyStructural BioinformaticsMolecular Modeling
ResearchPapers Link to Google Scholar
Teaching & PhD Courses Basic and advanced NMR - Level 1 A (EPFL) CH-601(x) Basic theoretical and experimental aspects of NMR will be taught. Students will be familarized with modern NMR spectrometers. Basic and advanced NMR - Level 2 (EPFL) CH-703 The goal is to give a sound theoretical an
Arne Seitz04/1996-06/2000 Scientific co-worker at Philipps-University of Marburg, Dep. of Physical Chemistry
07/2000-10/2002 Post Doc at Max-Planck Unit for Structural Molecular Biology in Hamburg
11/2002-11/2005 Post Doc at European Molecular Biolohy Laboratory (EMBL), Cell Biology and Cell Biophysics Programme
11/2005-03/2009 Staff Scientist at Advanced Light Microscopy Facility, EMBL
04/2009- Head of Bioimaging and Optics platforme (BIOP), Ecole Polytechnique Fédérale de Lausanne (EPFL)
Amine BenouhibaAmine Benouhiba graduated from the Preparatory School of Science and Technology, Algeria, in 2012. He received his engineering degree in material engineering from Ecole Nationale Polytechnique (ENP), Algeria, in 2015. He also received his master’s degree in innovative materials from Ecole centrale de Lyon (ECL) in 2016. He completed his Ph.D. degree in 2020 at the Bourgogne-Franche-Comté University. Currently, he is a postdoctoral researcher at Ecole polytechnique fédérale de Lausanne (EPFL). His research interests include the design and development of soft robotics, origami-inspired structures and microrobotics.
Yves PerriardYves Perriard was born in Lausanne in 1965. He received the M. Sc. in Microengineering from the Swiss Federal Institute of Technology - Lausanne (EPFL) in 1989 and the Ph D. degree in 1992. Co-founder of Micro-Beam SA, he was CEO of this company involved in high precision electric drive. Senior lecturer from 1998 and professor since 2003, he is currently director of Laboratory of Integrated Actuators. His research interests are in the field of new actuator design and associated electronic devices. In 2009, he is appointed Vice-Director of the Microengineering Institute in Neuchâtel until 2011. In 2013 the Federal Council has named him the the CTI commission in Bern. In 2014 he is appointed guest professor at Zhejiang University in China. In 2017, the lab is granted by the Werner Siemens Foundation of an amount of 12 millions CHF in order to set up a new Center for Artificial Muscules. Since 2018, he is Expert with Innosuisse, the new Swiss Innovation Agency. http://scholar.google.com/citations?hl=fr&user=V2onuO8AAAAJ https://actu.epfl.ch/news/a-12-million-franc-donation-to-create-a-center-for/ Yoan René Cyrille CivetTeaching & PhD PhD Students Simon Holzer Past EPFL PhD Students as codirector Dan Shi, Jasha Poliakine, Jonathan Chavanne, Quentin Philippe Mario De Menech Courses Electrotechnics I MICRO-100 The course covers the basics of electrical circuits composed of linear components, in direct current (DC) operation. A series of transforming methods are studied. At the end of the semester, alternate current (AC) systems are treated (complex numbers). Many examples and demos illustrate the course.
Giovanni De MicheliExpertise
Design technologies for integrated systems on silicon, such as synthesis, hw/sw codesign and low-power design, as well as systems on heterogeneous platforms including electrical and biological components.
Scientific Director of EcoCloud Center at EPFL
Directing activities towards creating sustainable computation including AI.Designing energy-savvy data centers and edge devices.
More information
Please read http://si2.epfl.ch/~demichel/
Giovanni De Micheli is a research scientist in electronics and Lucia Baldi Unser01/2021 - present Deputy to the Associate VP for Research / Deputy to the Associate VP for Centers & Platforms, EPFL06/2017 - 12/2020 Deputy to the Dean, School of Basic Sciences, Ecole Polytechnique Fédérale de Lausanne ;01/2015 - 11/2017 Lecturer/Communication and outreach manager at the School of Life Sciences, EPFL04/1998 - 03/2015: Research and Teaching Associate/Lecturer, Laboratory of Cellular Biotechnology, School of Life Sciences; 01/1994 - 07/1997: Visiting Fellow, Laboratory of Immunoregulation, NIAID, NIH, Bethesda, Maryland, USA;1991 - 1994: Research Fellow, Laboratory of Pediatric Oncology, G. Gaslini Scientific Institute, Genova, Italy;1987 - 1990: Research Fellow, Clinical Immunology and Immunogenetics Dept., Nat. Inst. for Cancer Research, Genova, Italy.
Henry MarkramHenry Markram started a dual scientific and medical career at the University of Cape Town, in South Africa. His scientific work in the 80s revealed the polymodal receptive fields of pontomedullary reticular formation neurons in vivo and how acetylcholine re-organized these sensory maps.
He moved to Israel in 1988 and obtained his PhD at the Weizmann Institute where he discovered a link between acetylcholine and memory mechanisms by being the first to show that acetylcholine modulates the NMDA receptor in vitro studies, and thereby gates which synapses can undergo synaptic plasticity. He was also the first to characterize the electrical and anatomical properties of the cholinergic neurons in the medial septum diagonal band.
He carried out a first postdoctoral study as a Fulbright Scholar at the NIH, on the biophysics of ion channels on synaptic vesicles using sub-fractionation methods to isolate synaptic vesicles and patch-clamp recordings to characterize the ion channels. He carried out a second postdoctoral study at the Max Planck Institute, as a Minerva Fellow, where he discovered that individual action potentials propagating back into dendrites also cause pulsed influx of Ca2 into the dendrites and found that sub-threshold activity could also activated a low threshold Ca2 channel. He developed a model to show how different types of electrical activities can divert Ca2 to activate different intracellular targets depending on the speed of Ca2 influx an insight that helps explain how Ca2 acts as a universal second messenger. His most well known discovery is that of the millisecond watershed to judge the relevance of communication between neurons marked by the back-propagating action potential. This phenomenon is now called Spike Timing Dependent Plasticity (STDP), which many laboratories around the world have subsequently found in multiple brain regions and many theoreticians have incorporated as a learning rule. At the Max-Planck he also started exploring the micro-anatomical and physiological principles of the different neurons of the neocortex and of the mono-synaptic connections that they form - the first step towards a systematic reverse engineering of the neocortical microcircuitry to derive the blue prints of the cortical column in a manner that would allow computer model reconstruction.
He received a tenure track position at the Weizmann Institute where he continued the reverse engineering studies and also discovered a number of core principles of the structural and functional organization such as differential signaling onto different neurons, models of dynamic synapses with Misha Tsodyks, the computational functions of dynamic synapses, and how GABAergic neurons map onto interneurons and pyramidal neurons. A major contribution during this period was his discovery of Redistribution of Synaptic Efficacy (RSE), where he showed that co-activation of neurons does not only alter synaptic strength, but also the dynamics of transmission. At the Weizmann, he also found the tabula rasa principle which governs the random structural connectivity between pyramidal neurons and a non-random functional connectivity due to target selection. Markram also developed a novel computation framework with Wolfgang Maass to account for the impact of multiple time constants in neurons and synapses on information processing called liquid computing or high entropy computing.
In 2002, he was appointed Full professor at the EPFL where he founded and directed the Brain Mind Institute. During this time Markram continued his reverse engineering approaches and developed a series of new technologies to allow large-scale multi-neuron patch-clamp studies. Markrams lab discovered a novel microcircuit plasticity phenomenon where connections are formed and eliminated in a Darwinian manner as apposed to where synapses are strengthening or weakened as found for LTP. This was the first demonstration that neural circuits are constantly being re-wired and excitation can boost the rate of re-wiring.
At the EPFL he also completed the much of the reverse engineering studies on the neocortical microcircuitry, revealing deeper insight into the circuit design and built databases of the blue-print of the cortical column. In 2005 he used these databases to launched the Blue Brain Project. The BBP used IBMs most advanced supercomputers to reconstruct a detailed computer model of the neocortical column composed of 10000 neurons, more than 340 different types of neurons distributed according to a layer-based recipe of composition and interconnected with 30 million synapses (6 different types) according to synaptic mapping recipes. The Blue Brain team built dozens of applications that now allow automated reconstruction, simulation, visualization, analysis and calibration of detailed microcircuits. This Proof of Concept completed, Markrams lab has now set the agenda towards whole brain and molecular modeling.
With an in depth understanding of the neocortical microcircuit, Markram set a path to determine how the neocortex changes in Autism. He found hyper-reactivity due to hyper-connectivity in the circuitry and hyper-plasticity due to hyper-NMDA expression. Similar findings in the Amygdala together with behavioral evidence that the animal model of autism expressed hyper-fear led to the novel theory of Autism called the Intense World Syndrome proposed by Henry and Kamila Markram. The Intense World Syndrome claims that the brain of an Autist is hyper-sensitive and hyper-plastic which renders the world painfully intense and the brain overly autonomous. The theory is acquiring rapid recognition and many new studies have extended the findings to other brain regions and to other models of autism.
Markram aims to eventually build detailed computer models of brains of mammals to pioneer simulation-based research in the neuroscience which could serve to aggregate, integrate, unify and validate our knowledge of the brain and to use such a facility as a new tool to explore the emergence of intelligence and higher cognitive functions in the brain, and explore hypotheses of diseases as well as treatments.
