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Concept

Primary immunodeficiency

Related publications (25)

Hyperbranched Polylysine: A Versatile, Biodegradable Transfection Agent for the Production of Recombinant Proteins by Transient Gene Expression and the Transfection of Primary Cells

Florian Maria Wurm, Harm-Anton Klok, David Hacker, Lucia Baldi Unser, Yashas Rajendra, Zuzana Kadlecova

The feasibility of a new transfection agent, HBPL, for the production of recombinant IgG antibody via TGE as well as for the transfection of primary cells is studied. Under the conditions investigated, transfection of CHO-DG44 cells using HBPL results in I ...

2012

Cell viability assessment by flow cytometry using yeast as cell model

Philippe Renaud, Niccolo Piacentini, Nina Buffi

This paper reports the new combination of cell sorting and counting capabilities on a single device. Most state-of-the-art devices combining these technologies use optical techniques requiring complicated experimental setups and labeled samples. The use of ...

Elsevier2011

Gene Delivery with Hyperbranched Polylysine

Zuzana Kadlecova

The delivery of exogenous nucleic acids into mammalian cells is a valuable technique for basic research studies on the expression, regulation and function of genes and proteins. At the same time, gene delivery to cultivated mammalian cells has become funda ...

EPFL2011

Identification of genetic insulator elements to increase the safety of viral gene therapy vectors

Haematopoeitic system diseases, both acquired and inherited, can be currently cured by allogeneic haematopoietic stem cell transplantation. This treatment provides highly successful immune function recovery for patients receiving grafts of HLA-compatible d ...

EPFL2010

Elucidating the Role of Quaternary Structure in Modulating Macrophage Migration Inhibitory Factor (MIF) Functions

Macrophage Migration Inhibitory Factor (MIF) is a multifunctional protein and a major mediator of innate immunity. The paramount importance of MIF in the pathogenesis of many inflammatory, infectious, auto-immune and oncogenic diseases led to its emergence ...

EPFL2010