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Concept

Stable isotope labeling by amino acids in cell culture

Related publications (11)

Progress and pitfalls of using isobaric mass tags for proteome profiling

Loïc Dayon

Introduction: Quantitative proteomics using mass spectrometry is performed via label-free or label-based approaches. Labeling strategies rely on the incorporation of stable heavy isotopes by metabolic, enzymatic, or chemical routes. Isobaric labeling uses ...

TAYLOR & FRANCIS LTD2020

Cryogenic infrared spectroscopy as a probe of structure and dynamics of biomolecules

Valeriu Scutelnic

The pivotal theory of molecular biology states that the structure of biomolecules is directly related to their function in living systems. In this way, the structural investigation of biological molecules allows understanding and intervening in the fundame ...

EPFL2018

Using NanoSIMS coupled with microfluidics to visualize the early stages of coral infection by Vibrio coralliilyticus

Anders Meibom, Isabelle Domart-Coulon, Emma Mary Gibbin

Background: Global warming has triggered an increase in the prevalence and severity of coral disease, yet little is known about coral/pathogen interactions in the early stages of infection. The point of entry of the pathogen and the route that they take on ...

2018

Identification of HcgC as a SAM-Dependent Pyridinol Methyltransferase in [Fe]-Hydrogenase Cofactor Biosynthesis

Xile Hu, Tao Xu, Lichen Bai

Previous retrosynthetic and isotope-labeling studies have indicated that biosynthesis of the iron guanylylpyridinol (FeGP) cofactor of [Fe]-hydrogenase requires a methyltransferase. This hypothetical enzyme covalently attaches the methyl group at the 3-pos ...

Wiley-Blackwell2016

Gas-phase probes of kinetically trapped peptides

Liudmila Voronina

Gas-phase techniques are promising for studying intrinsically disordered peptides and proteins, as they can be implemented in a conformer-selective way. One of the issues that prevent them from finding a broader use is the question of to which extent the s ...

EPFL2016

A nanoscale secondary ion mass spectrometry study of dinoflagellate functional diversity in reef-building corals

Anders Meibom, Isabelle Domart-Coulon

Nutritional interactions between corals and symbiotic dinoflagellate algae lie at the heart of the structural foundation of coral reefs. Whilst the genetic diversity of Symbiodinium has attracted particular interest because of its contribution to the sensi ...

Wiley-Blackwell2015

Redox variations and bioproductivity in the Ediacaran: Evidence from inorganic and organic geochemistry of the Corumba Group, Brazil

Mariluz Bagnoud

Stable isotope ratios combined with elemental compositions and molecular biomass data provide a powerful tool in Neoproterozoic palaeoenvironmental interpretations. Here, we report the results of an extensive organic and inorganic geochemical study perform ...

Elsevier2014

Closing the gap between T-cell life span estimates from stable isotope-labeling studies in mice and humans

Istvan Bartha

Quantitative knowledge of the turnover of different leukocyte populations is a key to our understanding of immune function in health and disease. Much progress has been made thanks to the introduction of stable isotope labeling, the state-of-the-art techni ...

Amer Soc Hematology2013

Quantification of biodegradation for o-xylene and naphthalene using first order decay models, Michaelis-Menten kinetics and stable carbon isotopes

At a former wood preservation plant severely contaminated with coal tar oil, in situ bulk attenuation and biodegradation rate constants for several monoaromatic (BTEX) and polyaromatic hydrocarbons (PAH) were determined using (1) classical first order deca ...

2009

Diffusion Coefficients of Biomolecules Using Long-Lived Spin States

Geoffrey Bodenhausen, Riddhiman Sarkar, Paul Romeo Vasos, Puneet Ahuja

We report the first observation of long-lived states (LLS) having lifetimes TLLS that exceed the corresponding spin−lattice relaxation times T1 by more than a factor 6 in a protein. Slow diffusion coefficients characteristic of large biomolecules can be de ...

2009

Kinetic studies on the first dihydrogen aquacomplex, [Ru(H2)(H2O)5]2+: Formation under H2 pressure and catalytic H/D isotope exchange in water

André Merbach, Lothar Helm, Gabor Laurenczy, Oleg Yazyev, Pascal Grundler

The ruthenium(II) hexaaqua complex [Ru(H2O)6]2+ reacts with dihydrogen under pressure to give the h2-dihydrogen ruthenium(II) pentaaqua complex [Ru(H2)(H2O)5]2+.The complex was characterized by 1H, 2H and 17O NMR: d H = -7.65 ppm, J HD = 31.2 Hz, d O = -80 ...

2006