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Concept

Hyperpolarization (biology)

Related publications (27)

Ricca's factors as mobile proteinaceous effectors of electrical signaling

Jian Wang, Satyam Tiwari

Leaf-feeding insects trigger high-amplitude, defense-inducing electrical signals called slow wave potentials (SWPs). These signals are thought to be triggered by the long-distance transport of low molecular mass elicitors termed Ricca's factors. We sought ...

CELL PRESS2023

Design and performance of a small bath cryostat with NMR capability for transport of hyperpolarized samples

Andrea Capozzi

As of today, dissolution Dynamic Nuclear Polarization (dDNP) is the only clinically available hyperpolarization technique for C-13-MRI. Despite the clear path towards personalized medicine that dDNP is paving as an alternative and/or complement to Positron ...

Nature Portfolio2022

Cell Type-Specific Membrane Potential Changes in Dorsolateral Striatum Accompanying Reward-Based Sensorimotor Learning

Carl Petersen, Sylvain Crochet, Tanya C Sippy

The striatum integrates sensorimotor and motivational signals, likely playing a key role in reward-based learning of goal-directed behavior. However, cell type-specific mechanisms underlying reinforcement learning remain to be precisely determined. Here, w ...

OXFORD UNIV PRESS2021

Scaling analyses for hyperpolarization transfer across a spin-diffusion barrier and into bulk solid media

David Lyndon Emsley, Arthur César Pinon

By analogy to heat and mass transfer film theory, a general approach is introduced for determining hyperpolarization transfer rates between dilute electron spins and a surrounding nuclear ensemble. These analyses provide new quantitative relationships for ...

2021

Deep learning-based reduced order models in cardiac electrophysiology

Alfio Quarteroni, Andrea Manzoni, Luca Dede'

Predicting the electrical behavior of the heart, from the cellular scale to the tissue level, relies on the numerical approximation of coupled nonlinear dynamical systems. These systems describe the cardiac action potential, that is the polarization/depola ...

2020

A stick-slip/inchworm hybrid rotary piezo motor based on a symmetric triangular driving mechanism

Dongdong Zheng

A stick-slip/inchworm hybrid rotary piezomotor based on a symmetric triangular driving mechanism, which can simultaneously achieve the benefits of both stick-slip and inchworm motors, was reported in this letter. It is based on the principle of stick-slip ...

2019

Nonlinear microscopy for temporally and spatially resolved label-free imaging of living cells

Marie Eve Pascale Didier

The human brain consists of approximately a hundred billion of neurons that communicate through a unique series of biochemical and electrical processes. When neuronal networks are solicited, electrochemical processes happen involving millions of cells with ...

EPFL2018

Transportable hyperpolarized metabolites

Geoffrey Bodenhausen, David Lyndon Emsley, Sami Jannin, Aurélien Bornet, Jonas Milani, Basile Vuichoud, Aaron James Rossini, Xiao Ji

Nuclear spin hyperpolarization of C-13-labelled metabolites by dissolution dynamic nuclear polarization can enhance the NMR signals of metabolites by several orders of magnitude, which has enabled in vivo metabolic imaging by MRI. However, because of the s ...

Nature Publishing Group2017

Intercellular ultrafast Ca2+ wave in vascular smooth muscle cells: numerical and experimental study

Jean-Jacques Meister, Niccolo Piacentini, Jairo Camilo Quijano Perez, Franck Claude Raynaud

Vascular smooth muscle cells exhibit intercellular Ca2+ waves in response to local mechanical or KCl stimulation. Recently, a new type of intercellular Ca2+ wave was observed in vitro in a linear arrangement of smooth muscle cells. The intercellular wave w ...

Nature Research2016

Filterable Agents for Hyperpolarization of Water, Metabolites, and Proteins

Geoffrey Bodenhausen, Sami Jannin, Aurélien Bornet, Jonas Milani, Pascal Miéville, Basile Vuichoud, Florian Patrick Marie Daniel De la Barre De Nanteuil, Estel Canet Martinez, Xiao Ji

Hyperpolarization is generated by dissolution dynamic nuclear polarization (d-DNP) using a polymer-based polarizing agent dubbed FLAP (filterable labeled agents for polarization). It consists of a thermo-responsive poly(N-isopropylacrylamide), also known a ...

Wiley-V C H Verlag Gmbh2016

Hyperpolarized Water to Study Protein-Ligand Interactions

Geoffrey Bodenhausen, Sami Jannin, Aurélien Bornet, Jonas Milani, Basile Vuichoud

The affinity between a chosen target protein and small molecules is a key aspect of drug discovery. Screening by popular NMR methods such as Water-LOGSY suffers from low sensitivity and from false positives caused by aggregated or denatured proteins. This ...

2015

On the Firing Rate Dependency of the Phase Response Curve of Rat Purkinje Neurons In Vitro

Michele Giugliano

Synchronous spiking during cerebellar tasks has been observed across Purkinje cells: however, little is known about the intrinsic cellular mechanisms responsible for its initiation, cessation and stability. The Phase Response Curve (PRC), a simple input-ou ...

Public Library Science2015

The human CFTR protein expressed in CHO cells activates aquaporin-3 in a cAMP-dependent pathway: study by digital holographic microscopy

Pierre Magistretti, Pierre Marquet, Sylvain Eric Lengacher, Pascal Jourdain

The transmembrane water movements during cellular processes and their relationship to ionic channel activity remain largely unknown. As an example, in epithelial cells it was proposed that the movement of water could be directly linked to cystic fibrosis t ...

Company of Biologists2014

Single-cell RT-PCR, a technique to decipher the electrical, anatomical, and genetic determinants of neuronal diversity

Henry Markram

The patch-clamp technique has allowed for detailed studies on the electrical properties of neurons. Dye loading through patch pipettes enabled characterizing the morphological properties of the neurons. In addition, the patch-clamp technique also allows fo ...

Springer2014

Command-line cellular electrophysiology for conventional and real-time closed-loop experiments

Michele Giugliano

Background: Current software tools for electrophysiological experiments are limited in flexibility and rarely offer adequate support for advanced techniques such as dynamic clamp and hybrid experiments, which are therefore limited to laboratories with a si ...

Elsevier Science Bv2014

Ultrafast Ca2+ wave in cultured vascular smooth muscle cells aligned on a micropatterned surface

Jean-Jacques Meister, Jean-Louis Bény, Benoît Vianay, Jairo Camilo Quijano Perez

Communication between vascular smooth muscle cells (SMCs) allows control of their contraction and so regulation of blood flow. The contractile state of SMCs is regulated by cytosolic Ca2+ concentration (Ca2+) which propagates as Ca2+ waves over a sign ...

Elsevier2013

Intercellular ultrafast calcium wave in vascular smooth muscle cells

Jairo Camilo Quijano Perez

Communication between vascular smooth muscle cells (SMCs) allows control of their contraction and so regulation of blood flow. The contractile state of SMCs is controled by cytosolic Ca2+ concentration ([Ca2+]i) which propagates as Ca2+ waves over a signif ...

EPFL2013

Nlgn4 knockout induces network hypo-excitability in juvenile mouse somatosensory cortex in vitro

Henry Markram, Vincent Delattre, Kamila Markram, Julie Meystre, Deborah La Mendola

Neuroligins (Nlgns) are postsynaptic cell adhesion molecules that form transynaptic complexes with presynaptic neurexins and regulate synapse maturation and plasticity. We studied the impact of the loss of Nlgn4 on the excitatory and inhibitory circuits in ...

Nature Publishing Group2013

Automated transfer and injection of hyperpolarized molecules with polarization measurement prior to in vivo NMR

Arnaud Comment, Josefina Adriana Maria Bastiaansen, Tian Cheng, Mor-Miri Mishkovsky

Hyperpolarized magnetic resonance via dissolution dynamic nuclear polarization necessitates the transfer of the hyperpolarized molecules from the polarizer to the imager prior to in vivo measurements. This process leads to unavoidable losses in nuclear pol ...

2013

A computer-assisted multi-electrode patch-clamp system

Henry Markram, Rodrigo de Campos Perin

The patch-clamp technique is today the most well-established method for recording electrical activity from individual neurons or their subcellular compartments. Nevertheless, achieving stable recordings, even from individual cells, remains a time-consuming ...

Journal of Visualized Experiments2013