Concept

Embryo quality

Related publications (22)

Artificial embryonic node elucidates the role of flow in left-right symmetry breaking in vertebrates

During the embryonic development of vertebrates, initially, symmetric embryos develop asymmetrically arranged organs. The asymmetry initiates with the formation of a small fluid-filled cavity on the embryo called the embryonic node, which contains motile cilia that generate specific flow patterns. The mechanism by which this nodal flow is sensed and causes asymmetry development has remained elusive despite major experimental and computational efforts. Existing hypotheses focus on either mechanical or chemic

American Association for the Advancement of Science (AAAS)2026

Reconstituting Mouse Embryogenesis Ex Utero from Gastrulation to Fetal Development Reveals Maternally Independent Metabolic Programs

Mammalian development takes place inside the maternal uterus, creating technological constraints that make difficult the study of embryogenesis in live developing embryos. A central challenge for understanding the role of metabolism in mammalian development is discriminating placental and uterine-regulated signals from embryo-intrinsic processes independent of maternal influence, a process that until now has remained inseparable during gastrulation and organogenesis 1–3 . Ex utero culture systems allowing c

bioRxiv2026

Unified mass imaging maps the lipidome of vertebrate development

Embryo development entails the formation of anatomical structures with distinct biochemical compositions. Compared with the wealth of knowledge on gene regulation, our understanding of metabolic programs operating during embryogenesis is limited. Mass spectrometry imaging (MSI) has the potential to map the distribution of metabolites across embryo development. Here we established uMAIA, an analytical framework for the joint analysis of large MSI datasets, which enables the construction of multidimensional m

Nature Publishing Group2025

C. elegans SAS-1 ensures centriole integrity and ciliary function, and operates with SSNA-1

Centrioles are microtubule-based organelles critical for signaling, motility and division. The microtubule-binding protein SAS-1 is homologous to the human ciliopathy component C2CD3 and contributes to centriole integrity in C. elegans , but how this function is exerted is incompletely understood. Here, through the generation of a null allele and analysis with U-Ex-STED, we establish that SAS-1 is dispensable for the onset of centriole assembly, but essential for organelle integrity during oogenesis, sperma

Public Library of Science (PLoS)2025

DPPA2 and DPPA4 are necessary to establish a 2C‐like state in mouse embryonic stem cells

Didier Trono, Julien Léonard Duc, Alexandre Coudray, Alberto De Iaco

After fertilization of the transcriptionally silent oocyte, expression from both parental chromosomes is launched through zygotic genome activation (ZGA), occurring in the mouse at the 2-cell (2C) stage. Among the first elements to be transcribed are the D ...

2019

Segmentation of Developing Human Embryo in Time-lapse Microscopy

Mathieu Salzmann

Being able to efficiently segment a developing embryo from background clutter constitutes an important step in automated monitoring of human embryonic cells. State-of-the-art automatic segmentation methods remain ill-suited to handle the complex behavior a ...

IEEE2016

Genetic depletion of Polo-like kinase 1 leads to embryonic lethality due to mitotic aberrancies

Polo-like kinase 1 (PLK1) is a serine/threonine kinase that plays multiple and essential roles during the cell division cycle. Its inhibition in cultured cells leads to severe mitotic aberrancies and cell death. Whereas previous reports suggested that Plk1 ...

Wiley-Blackwell2016

Multi-dimensional imaging and phenotyping of C. elegans embryos via an automated microfluidic device

Martinus Adela Maria Gijs, Thomas Lehnert

We introduce a microfluidic platform for automated on-chip worm culture, creation of synchronized embryo microarrays, and long-term parallel live imaging of Caenorhabditis elegans embryos. Our device allows studying well-defined embryo populations at unprecedented spatio-temporal resolution, via fully automated multi-dimensional imaging, covering six independent dimensions: the 3 spatial coordinates, development time, exposure duration/type (brightfield, fluorescent), and embryo number in the microarray. We

2015

Biomechanical forces promote blood development through prostaglandin E2 and the cAMP-PKA signaling axis

Olaia Maria Naveiras Torres-Quiroga, Nan Li

Blood flow promotes emergence of definitive hematopoietic stem cells (HSCs) in the developing embryo, yet the signals generated by hemodynamic forces that influence hematopoietic potential remain poorly defined. Here we show that fluid shear stress endows ...

2015

TRIM28 repression of retrotransposon-based enhancers is necessary to preserve transcriptional dynamics in embryonic stem cells

TRIM28 is critical for the silencing of endogenous retroviruses (ERVs) in embryonic stem (ES) cells. Here, we reveal that an essential impact of this process is the protection of cellular gene expression in early embryos from perturbation by cis-acting activators contained within these retroelements. In TRIM28-depleted ES cells, repressive chromatin marks at ERVs are replaced by histone modifications typical of active enhancers, stimulating transcription of nearby cellular genes, notably those harboring biv

2013

The transcriptional landscape of hematopoietic stem cell ontogeny

Olaia Maria Naveiras Torres-Quiroga

Transcriptome analysis of adult hematopoietic stem cells (HSCs) and their progeny has revealed mechanisms of blood differentiation and leukemogenesis, but a similar analysis of HSC development is lacking. Here, we acquired the transcriptomes of developing ...

2012

Atopic dermatitis-like disease and associated lethal myeloproliferative disorder arise from loss of notch signaling in the murine skin

BACKGROUND: The Notch pathway is essential for proper epidermal differentiation during embryonic skin development. Moreover, skin specific loss of Notch signaling in the embryo results in skin barrier defects accompanied by a B-lymphoproliferative disease. However, much less is known about the consequences of loss of Notch signaling after birth. METHODOLOGY AND PRINCIPAL FINDINGS: To study the function of Notch signaling in the skin of adult mice, we made use of a series of conditional gene targeted mice th

Public Library of Science2010

Quantitative analysis of gene regulation in Drosophila

In this project, characterization of the MAPK signaling cascade was done using the terminal patterning of Drosophila embryo as an experimental system. MAPK acts downstream of the Torso receptor tyrosine kinase (RTK) whose activation is limited to the poles of the embryo. This results in a localized activation of MAPK which is essential for the specification of the embryonic termini. Active MAPK controls the expression of the tailless (tll) and huckebein (hkb) gap genes by downregulating a transcriptional re

2010

Exploration of signaling cycles using dynamic optimization

Dominique Bonvin

One of the basic characteristics of every living system is the ability to respond to extracellular signals. This is carried out through a limited number of protein-based signaling networks, whose function is not based only on simple transmission of the received signals, but incorporates the processing, encoding and integration of both, external and internal signals. The results than lead to different changes in gene expression, regulate cell growth, differentiation, embryo development, and stress responses

2010

Exploration of Signaling Cycles using Dynamic Optimization

Dominique Bonvin

One of the basic characteristics of every living system is the ability to respond to extracellular signals. This is carried out through a limited number of protein-based signaling networks, whose function is not only based on simple transmission of the received signals, but incorporates the processing, encoding and integration of both external and internal signals. The results than lead to different changes in gene expression, regulate cell growth, mitogenesis, differentiation, embryo development, and stres

2010

Vascular effects induced by anti-VEGF agents in the CAM model: Effect of the DMSO

Hubert van den Bergh, Georges Wagnières

The chicken embryo's chorioallantoic membrane (CAM) is widely used as an in vivo model to study the vascular effects induced by agents administrated topically or intravenously. Hence, in the vascular plexus of this respiratory membrane, angiogenic and anti-angiogenic agents, as well as phototoxic effects have been studied. The main goal of this study was to characterize the capillary network of the CAM after topical administration of dimethyl sulfoxid (DMSO), a frequently used solvent of lipophylic drugs, i

2009

Characterization of pip5k3 fleck corneal dystrophy-linked gene in zebrafish

PIKfyve is a kinase encoded by pip5k3 involved in phosphatidylinositols (PdtIns) pathways. These lipids building cell membranes have structural functions and are involved in complex intracellular regulations. Mutations in human PIP5K3 are associated with F ...

2008

Zyg-11 and cul-2 regulate progression through meiosis II and polarity establishment in C. elegans

The mechanisms that ensure coupling between meiotic cell cycle progression and subsequent developmental events, including specification of embryonic axes, are poorly understood. Here, we establish that zyg-11 and the cullin cul-2 promote the metaphase-to-anaphase transition and M phase exit at meiosis II in Caenorhabditis elegans. Our results indicate that ZYG-11 acts with a CUL-2-based E3 ligase that is essential at meiosis II and that functions redundantly with the anaphase-promoting complex/cyclosome at

2004

RIC-8 is required for GPR-1/2-dependent Galpha function during asymmetric division of C. elegans embryos

Heterotrimeric G proteins are crucial for asymmetric cell division, but the mechanisms of signal activation remain poorly understood. Here, we establish that the evolutionarily conserved protein RIC-8 is required for proper asymmetric division of one-cell stage C. elegans embryos. Spindle severing experiments demonstrate that RIC-8 is required for generation of substantial pulling forces on astral microtubules. RIC-8 physically interacts with GOA-1 and GPA-16, two Galpha subunits that act in a partially red

2004

Differential activation of the DNA replication checkpoint contributes to asynchrony of cell division in C. elegans embryos

BACKGROUND: Acquisition of lineage-specific cell cycle duration is a central feature of metazoan development. The mechanisms by which this is achieved during early embryogenesis are poorly understood. In the nematode Caenorhabditis elegans, differential cell cycle duration is apparent starting at the two-cell stage, when the larger anterior blastomere AB divides before the smaller posterior blastomere P(1). How anterior-posterior (A-P) polarity cues control this asynchrony remains to be elucidated.RESULTS:

2003

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Related publications (22)

Artificial embryonic node elucidates the role of flow in left-right symmetry breaking in vertebrates

Yves Bellouard

American Association for the Advancement of Science (AAAS)2026

Reconstituting Mouse Embryogenesis Ex Utero from Gastrulation to Fetal Development Reveals Maternally Independent Metabolic Programs

bioRxiv2026

Unified mass imaging maps the lipidome of vertebrate development

Nature Publishing Group2025

C. elegans SAS-1 ensures centriole integrity and ciliary function, and operates with SSNA-1

Public Library of Science (PLoS)2025

DPPA2 and DPPA4 are necessary to establish a 2C‐like state in mouse embryonic stem cells

Didier Trono, Julien Léonard Duc, Alexandre Coudray, Alberto De Iaco

2019

Segmentation of Developing Human Embryo in Time-lapse Microscopy

Mathieu Salzmann

IEEE2016

Genetic depletion of Polo-like kinase 1 leads to embryonic lethality due to mitotic aberrancies

Wiley-Blackwell2016

Multi-dimensional imaging and phenotyping of C. elegans embryos via an automated microfluidic device

Martinus Adela Maria Gijs, Thomas Lehnert

2015

Biomechanical forces promote blood development through prostaglandin E2 and the cAMP-PKA signaling axis

Olaia Maria Naveiras Torres-Quiroga, Nan Li

2015

TRIM28 repression of retrotransposon-based enhancers is necessary to preserve transcriptional dynamics in embryonic stem cells

2013

The transcriptional landscape of hematopoietic stem cell ontogeny

Olaia Maria Naveiras Torres-Quiroga

2012

Atopic dermatitis-like disease and associated lethal myeloproliferative disorder arise from loss of notch signaling in the murine skin

Public Library of Science2010

Quantitative analysis of gene regulation in Drosophila

2010

Exploration of signaling cycles using dynamic optimization

Dominique Bonvin

2010

Exploration of Signaling Cycles using Dynamic Optimization

Dominique Bonvin

One of the basic characteristics of every living system is the ability to respond to extracellular signals. This is carried out through a limited number of protein-based signaling networks, whose function is not only based on simple transmission of the received signals, but incorporates the processing, encoding and integration of both external and internal signals. The results than lead to different changes in gene expression, regulate cell growth, mitogenesis, differentiation, embryo development, and stres

2010

Differential activation of the DNA replication checkpoint contributes to asynchrony of cell division in C. elegans embryos

2003