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Concept

Reverse genetics

Related publications (10)

Diet modulates cecum bacterial diversity and physiological phenotypes across the BXD mouse genetic reference population

Johan Auwerx, Evan Graehl Williams

The BXD family has become one of the preeminent genetic reference populations to understand the genetic and environmental control of phenotypic variation. Here we evaluate the responses to different levels of fat in the diet using both chow diet (CD, 13-18 ...

2019

Genetic characterization of an adapted pandemic 2009 H1N1 influenza virus that reveals improved replication rates in human lung epithelial cells

Christian Sieben

The 2009 influenza pandemic originated from a swine-origin H1N1 virus, which, although less pathogenic than anticipated, may acquire additional virulence-associated mutations in the future. To estimate the potential risk, we sequentially passaged the isola ...

Academic Press Inc Elsevier Science2016

Dicer1 imparts essential survival cues in Notch-driven T-ALL via miR-21-mediated tumor suppressor Pdcd4 repression

Freddy Radtke, Ute Koch, Fabian Junker

The modulatory function of individual microRNAs (miRNAs) in Notch-driven T-cell acute lymphoblastic leukemias (T-ALLs) has recently been established. Although protumorigenic and tumor-suppressive miRNAs are implicated in disease onset in murine models of N ...

American Society of Hematology2015

The impact of host genetic variation on infection with HIV-1

Paul Jack Mc Laren

The outcome after infection with the human immunodeficiency virus type 1 (HIV-1) is a complex phenotype determined by interactions among the pathogen, the human host and the surrounding environment. An impact of host genetic variation on HIV-1 susceptibili ...

Nature Publishing Group2015

TransFLP - A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination

Melanie Blokesch

Several methods are available to manipulate bacterial chromosomes(1-3). Most of these protocols rely on the insertion of conditionally replicative plasmids (e.g. harboring pir-dependent or temperature-sensitive replicons(1,2)). These plasmids are integrate ...

2012

Genetic manipulation of Vibrio cholerae by combining natural transformation with FLP recombination

Melanie Blokesch

Even though Vibrio cholerae is a well-known human pathogen, it is also a normal member of aquatic habitats. Within this environment it often forms biofilms on the chitin-containing exoskeleton of crustaceans and their molts. Chitin not only serves as nutri ...

Elsevier2010

Layer- and column-specific knockout of NMDA receptors in pyramidal neurons of the mouse barrel cortex

Carl Petersen

Viral vectors injected into the mouse brain offer the possibility for localized genetic modifications in a highly controlled manner. Lentivector injection into mouse neocortex transduces cells within a diameter of approximately 200mum, which closely matche ...

Frontiers Research Foundation2007

Bio-mimetic Evolutionary Reverse Engineering of Genetic Regulatory Networks

Dario Floreano, Claudio Mattiussi, Daniel Marbach

The effective reverse engineering of biochemical networks is one of the great challenges of systems biology. The contribution of this paper is two-fold: 1) We introduce a new method for reverse engineering genetic regulatory networks from gene expression d ...

Springer-Verlag Berlin2007

Viral-based modelling and correction of neurodegenerative diseases by RNA interference

Patrick Aebischer

Experimental recapitulation of recessive human genetic neurodegenerative disease in rodents can be classically addressed through genetic disruption of the related gene. Although very informative, this specific gene targeting is restricted to mice and precl ...

2006

Fast directed evolution of non-immunoglobulin proteins by somatic hypermutation in immune cells

Kai Johnsson, Christian Heinis

A review. New, improved phenotypes. Somatic hypermutation in immune cells can be used for the fast directed evolution of proteins other than Igs (e.g., autofluorescent proteins). The target gene is inserted into the genome of activated B lymphocytes where ...

2005