Concept

Glutamine synthetase

Related publications (20)

A conditional glutamatergic synaptic vesicle marker for Drosophila

Glutamate is a principal neurotransmitter used extensively by the nervous systems of all vertebrate and invertebrate animals. It is primarily an excitatory neurotransmitter that has been implicated in nervous system development, as well as a myriad of brai ...

OXFORD UNIV PRESS INC2022

Magnetic Resonance Spectroscopy in Schizophrenia: Evidence for Glutamatergic Dysfunction and Impaired Energy Metabolism

Lijing Xin, João Miguel das Neves Duarte

In the past couple of decades, major efforts were made to increase reliability of metabolic assessments by magnetic resonance methods. Magnetic resonance spectroscopy (MRS) has been valuable for providing in vivo evidence and investigating biomarkers in ne ...

2019

Evolution of the neurochemical profiles in the G93A-SOD1 mouse model of amyotrophic lateral sclerosis

Rolf Gruetter, Patrick Aebischer, Hongxia Lei, Bernard Schneider, Carole Poitry-Yamate, Elisabeth Anne Dirren

In vivo (1)H magnetic resonance spectroscopy ((1)H-MRS) investigations of amyotrophic lateral sclerosis (ALS) mouse brain may provide neurochemical profiles and alterations in association with ALS disease progression. We aimed to longitudinally follow neur ...

2019

Alterations of Brain Energy Metabolism in Type 2 Diabetic Goto-Kakizaki Rats Measured In Vivo by (13)C Magnetic Resonance Spectroscopy

João Miguel das Neves Duarte, Rolf Gruetter, Sarah Catherine Sonnay

Type 2 diabetes (T2D) is associated with deterioration of brain structure and function. Here, we tested the hypothesis that T2D induces a reorganization of the brain metabolic networks that support brain function. For that, alterations of neuronal and glia ...

2019

Self-organization of parS centromeres by the ParB CTP hydrolase

Paolo De Los Rios, Stefano Zamuner

ParABS systems facilitate chromosome segregation and plasmid partitioning in bacteria and archaea. ParB protein binds centromeric parS DNA sequences and spreads to flanking DNA. We show that ParB is an enzyme that hydrolyzes cytidine triphosphate (CTP) to ...

American Association for the Advancement of Science2019

Glucocorticoid deficiency causes transcriptional and post-transcriptional reprogramming of glutamine metabolism

Benjamin Dieter Weger, Meltem Weger

Background: Deficient glucocorticoid biosynthesis leading to adrenal insufficiency is life-threatening and is associated with significant co-morbidities. The affected pathways underlying the pathophysiology of co-morbidities due to glucocorticoid deficienc ...

2018

Calcium phosphate formation on titanium-based surfaces: bioactivity evaluated by in vitro methods

Weitian Zhao

The prediction of implant behaviors in vivo by the use of easy-to-perform in vitro methods is of great interest in biomaterials research. In the field of bone implants, it is commonly accepted that a spontaneous formation of a hydroxyapatite (HA) layer on ...

EPFL2018

Feasibility of in vivo measurement of glucose metabolism in the mouse hypothalamus by (1) H-[(13) C] MRS at 14.1T

Rolf Gruetter, Hongxia Lei, João Miguel das Neves Duarte, Bernard Lanz, Antoine Timothée Cherix, Blanca Lizarbe Serra

PURPOSE: Determine the feasibility of (1) H-[(13) C] MRS in the mouse hypothalamus using a 14.1T magnet. METHODS: We optimized the design of a (1) H-[(13) C] surface coil to maximize the signal-to-noise ratio of (1) H-[(13) C] MRS in the mouse hypothalamus ...

Wiley2018

Energy metabolism in the rat cortex under thiopental anaesthesia measured

João Miguel das Neves Duarte, Rolf Gruetter, Nathalie Just, Sarah Catherine Sonnay

Barbiturates, commonly used as general anaesthetics, depress neuronal activity and thus cerebral metabolism. Moreover, they are likely to disrupt the metabolic support of astrocytes to neurons, as well as the uptake of nutrients from circulation. By employ ...

Wiley-Blackwell2017

MRS studies of neuroenergetics and glutamate/glutamine exchange in rats: Extensions to hyperammonemic models

Cristina Ramona Cudalbu Moldovan, Bernard Lanz, Veronika Rackayová

In vivo Magnetic Resonance Spectroscopy is a useful tool to characterize brain biochemistry as well as its alteration in a large number of major central nervous system diseases. The present review will focus on the study of the glutamate-glutamine cycle, a ...

Elsevier2017

Adenosine A(1) receptors control the metabolic recovery after hypoxia in rat hippocampal slices

João Miguel das Neves Duarte

Adenosine is a neuromodulator that protects neurons from hypoxia. This effect is attributed to the ability of adenosine A(1) receptors (A(1)R) to inhibit excitatory synaptic transmission. However, A(1)R activation also protects non-brain tissues from hypox ...

Wiley-Blackwell2016

Refined Analysis of Brain Energy Metabolism Using In Vivo Dynamic Enrichment of 13C Multiplets

João Miguel das Neves Duarte, Rolf Gruetter, Bernard Lanz, Nicolas Kunz, Masoumeh Dehghani Moghadam

Carbon-13 nuclear magnetic resonance spectroscopy in combination with the infusion of (13)C-labeled precursors is a unique approach to study in vivo brain energy metabolism. Incorporating the maximum information available from in vivo localized (13)C spect ...

Sage Publications Ltd2016

Assessment of metabolic fluxes in the mouse brain in vivo using (1)H-[(13)C] NMR spectroscopy at 14.1 Tesla

Rolf Gruetter, Lijing Xin, Hongxia Lei, Bernard Lanz

(13)C magnetic resonance spectroscopy (MRS) combined with the administration of (13)C labeled substrates uniquely allows to measure metabolic fluxes in vivo in the brain of humans and rats. The extension to mouse models may provide exclusive prospect for t ...

Nature Publishing Group2015

Optimized MEGA-SPECIAL for in vivo glutamine detection in the rat brain at 14.1 T

Rolf Gruetter, Cristina Ramona Cudalbu Moldovan, Mélanie Craveiro

Glutamine has multiple roles in brain metabolism and its concentration can be altered in various pathological conditions. An accurate knowledge of its concentration is therefore highly desirable to monitor and study several brain disorders in vivo. However ...

Wiley-Blackwell2014

Ammonia toxicity to the brain

Cristina Ramona Cudalbu Moldovan

Hyperammonemia can be caused by various acquired or inherited disorders such as urea cycle defects. The brain is much more susceptible to the deleterious effects of ammonium in childhood than in adulthood. Hyperammonemia provokes irreversible damage to the ...

Springer Verlag2013

Glutamatergic and GABAergic energy metabolism measured in the rat brain by 13C NMR spectroscopy at 14.1 T

João Miguel das Neves Duarte, Rolf Gruetter

Energy metabolism supports both inhibitory and excitatory neurotransmission processes. This study investigated the specific contribution of astrocytic metabolism to γ-aminobutyric acid (GABA) synthesis and inhibitory GABAergic neurotransmission that remain ...

Wiley-Blackwell2013

Development and implementation of ¹H magnetic resonance techniques for an improved in vivo assessment of models of cerebral metabolic disorders

Mélanie Craveiro

Nuclear magnetic resonance (NMR) is a physical phenomenon that is widely used in the biomedical field due to its non-invasive and non-destructive properties, which make it an optimal tool for the in vivo investigation of living organs such as the brain. Th ...

EPFL2013

Cerebral glutamine metabolism under hyperammonemia determined in vivo by localized H-1 and N-15 NMR spectroscopy

Rolf Gruetter, Cristina Ramona Cudalbu Moldovan, João Miguel das Neves Duarte, Bernard Lanz, Yves Pilloud

Brain glutamine synthetase (GS) is an integral part of the glutamate-glutamine cycle and occurs in the glial compartment. In vivo Magnetic Resonance Spectroscopy (MRS) allows noninvasive measurements of the concentrations and synthesis rates of metabolites ...

Nature Publishing Group2012

Reduced glutamine concentration improves protein production in growth-arrested CHO-DG44 and HEK-293E cells

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Yashas Rajendra, Divor Kiseljak

For most cultivated mammalian cells, glutamine is an essential medium component. However, glutamine consumption results in the production of ammonia, a cytotoxic byproduct. Here we investigated the effect of glutamine reduction on recombinant protein produ ...

Springer Netherlands2011

Effect of manganese chloride on the neurochemical profile of the rat hypothalamus

Rolf Gruetter, Cristina Ramona Cudalbu Moldovan, Hongxia Lei, Nathalie Just

Manganese (Mn 2+)-enhanced magnetic resonance imaging studies of the neuronal pathways of the hypothalamus showed that information about the regulation of food intake and energy balance circulate through specific hypothalamic nuclei. The dehydration-induce ...

Nature Publishing Group2011