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Concept

Restriction digest

Related publications (14)

PSGL-1 is an evolutionarily conserved antiviral restriction factor

Ying Liu

The arms race between viruses and their hosts shaped the evolutionary history and the genome composition of both parties. Restriction factors are the first-line antiviral effectors encoded by the host genomes and are often conserved through evolution to pr ...

AMER SOC MICROBIOLOGY2023

Establishing a Ternary System for Optical Monitoring of DNA-Protein Interactions with Single-Walled Carbon Nanotubes

Shang-Jung Wu

DNA-protein interactions lie at the crux of life's essential processes. As such, various technologies have been developed to characterize these interactions. The distinct advantages of these technologies can be leveraged to study different facets of these ...

EPFL2020

High-content, cell-by-cell assessment of HER2 overexpression and amplification: a tool for intratumoral heterogeneity detection in breast cancer

Martinus Adela Maria Gijs, Daniel Migliozzi, Huu Tuan Nguyen

Immunohistochemistry and fluorescence in situ hybridization are the two standard methods for human epidermal growth factor receptor 2 (HER2) assessment. However, they have severe limitations to assess quantitatively intratumoral heterogeneity (ITH) when mu ...

NATURE PUBLISHING GROUP2019

Restriction endonuclease AgeI is a monomer which dimerizes to cleave DNA

Virginija Jovaisaite

Although all Type II restriction endonucleases catalyze phosphodiester bond hydrolysis within or close to their DNA target sites, they form different oligomeric assemblies ranging from monomers, dimers, tetramers to higher order oligomers to generate a dou ...

Oxford University Press (OUP)2017

A Dirichlet problem involving the divergence operatord

Bernard Dacorogna, Gyula Csató

We consider the problem {div u + (a; u) = f in Omega u = u(0) on partial derivative Omega. We show that if curl a (x(0)) not equal for some x(0) epsilon Omega, then the problem is solvable without restriction on f. We also discuss the regularity of the sol ...

Elsevier Science Bv2016

Integrating Gene Synthesis and MITOMI for Rapid Protein Engineering

Matthew Christopher Blackburn

The research described within this thesis is primarily motivated by two fields of science with reversed, yet complementary, approaches to addressing the same essential objective: manipulating and understanding the complex program of life. Whereas synthetic ...

EPFL2016

Distributed Programming via Safe Closure Passing

Philipp Haller, Heather Christine Klug

Programming systems incorporating aspects of functional programming, e.g., higher-order func- tions, are becoming increasingly popular for large-scale distributed programming. New frameworks such as Apache Spark leverage functional techniques to provide hi ...

2015

Optimized distributed implementation of multiparty interactions with Restriction

Joseph Sifakis

Using high level coordination primitives allows enhanced expressiveness of component-based frameworks to cope with the inherent complexity of present-day systems designs. Nonetheless, their distributed implementation raises multiple issues, regarding both ...

Elsevier2015

Global Solutions to a Non-Local Diffusion Equation with Quadratic Non-Linearity

Joachim Krieger

In this paper we prove the global in time well-posedness of the following non-local diffusion equation with

\alpha\, \varepsilon [0, 2/3)

\partial_tu=\{(-\Delta)^{-1}u\}\Delta u+\alpha u^2, \,\, u(t=0)=u_0

. The initial condition

u_0

is positive, rad ...

2012

Assessing the Bacterial Ecology of Organohalide Respiration for the Design of Bioremediation Strategies

Noam Shani

Groundwater is essential for human activities and is sometimes referred to as a non-renewable resource in the same way as oil and gas. During the past century, precious groundwater reserves worldwide have been threatened by anthropogenic release of chemica ...

EPFL2012

The Interplay between Cytidine Deaminases, HIV and Domesticated Genetic Parasites

"There is more virus in us than us in us". John Coffin's famous sentence illustrates that particular nucleic acid sequences related to exogenous viruses, called retrotransposons, constitute almost half of the human genome and largely exceeds the amount of ...

EPFL2010

Innate Barriers to Genome Invasion : how TRIM5a Restricts Retroelements

Over the course of evolution, retroviruses and their hosts have been in constant conflict, resulting in a long-term evolutionary "arms race" in which host and pathogen alternately gain selective advantages through functional innovations. On the host side, ...

EPFL2010

Interfering residues narrow the spectrum of MLV restriction by human TRIM5alpha

Didier Trono, Priscilla Turelli, Séverine Reynard

TRIM5alpha is a restriction factor that limits infection of human cells by so-called N- but not B- or NB-tropic strains of murine leukemia virus (MLV). Here, we performed a mutation-based functional analysis of TRIM5alpha-mediated MLV restriction. Our resu ...

Public Library of Science2007

BTA, a novel reagent for DNA attachment on glass and efficient generation of solid-phase amplified DNA colonies

Gerardo Turcatti

The tricarboxylate reagent benzene-1,3,5-triacetic acid (BTA) was used to attach 5'-aminated DNA primers and templates on an aminosilanized glass surface for subsequent generation of DNA colonies by in situ solid-phase amplification. We have characterized ...

Oxford University Press2006