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Concept

Green fluorescent protein

Related lectures (32)

RNA-based Probes: Engineering GFP-mimics

Explores RNA-based probes, focusing on engineering GFP-mimics for tracking RNA, discussing chromophore maturation, Spinach evolution, and binding considerations.

Chemical Biology Tools: Transcriptome Interrogation

Covers chemical biology tools for RNA study and explores the applications of GFP in biosciences.

Microscopy Image Analysis

Explores light microscopy principles, fluorescent proteins, optical slicing, and modulation transfer functions in cellular imaging.

Fluorescence: Principles and Applications

Explores fluorescence principles, fluorophores, biomolecular analysis, and genetic engineering applications.

Plasmonic Biomarkers

Explores the use of plasmonic nanoparticles as robust biomarkers in sensing and microscopy applications.

Frontiers in Chem Bio: Bioluminescent Sensor Proteins

Covers bioluminescent sensor proteins for drug monitoring and the development of semi-synthetic sensors with self-labeling tags.

Flow Cytometry: An Invaluable Tool for Research and Clinical Applications

Introduces Flow Cytometry as a technology for rapid multi-parametric analysis of single cells in various disciplines.

Protein Purification Methods

Covers the purification of expressed proteins using methods like transformation, lysis, IMAC, and protein analysis.

Circular Permutation in Protein Engineering

Delves into circular permutation in protein engineering, focusing on optimizing proteins by introducing new termini without changing the structure.

PCR Testing for COVID-19

Explains the process of PCR testing for COVID-19 through genetic material amplification and fluorescent marker detection.

Bacteria GFP Expression

Discusses bacteria images under different conditions and the expression level change of a GFP-tagged protein.

Fluorescence: Applications in Photomedicine

Explores the principles of fluorescence lifetime and quenching in photomedicine.

Protein Purification: IMAC

Covers the purification of proteins using IMAC chromatography and analysis methods.

Stem Cell Differentiation Tools

Explores tools for stem cell biology, such as immunofluorescence, RNA FISH, and fluorescent proteins.

Short-read Sequencing Techniques MOOC: Neuroscience Reconstructed: Genetics and Brain Development

Covers short-read sequencing techniques using fluorescent dye and pyrosequencing.

Enzyme Inhibitors: Drug Design and Tools Log in to Mediaspace to watch this video

Explores enzyme inhibitors as drugs, drug design, and protein function, including activity-based protein profiling and genome probing tools.

RNA-Protein Interactions: PAR-CLIP Technology Log in to Mediaspace to watch this video

Explores PAR-CLIP technology for probing RNA-protein interactions and RNA sequences using chemical biology toolsets and 'Spinach' probes.

Fluorescence: Proteins & Applications Log in to Mediaspace to watch this video

Explores the discovery and applications of fluorescent proteins, with a focus on the revolutionary green fluorescent protein (GFP).

Fluorescence in Microscopy: Important Concepts Log in to Mediaspace to watch this video

Introduces fundamental concepts of fluorescence in microscopy, covering terms like excitation and emission spectra, Stokes shift, quantum efficiency, brightness, and photobleaching.

RNA-based probes: Spinach aptamers Log in to Mediaspace to watch this video

Explores RNA-based probes, focusing on Spinach aptamers as GFP-mimics of RNAs and their applications in model systems.