Concept

Transdifferentiation

Related publications (11)

Nanodiamond‐Based Quantum Sensing Reveals Changes in Endo‐Lysosomal Activity of Stimulated Cardiac Fibroblasts in a Cellular Fibrosis Model

Despite a growing recognition of endo‐lysosomes as key regulators of cellular homeostasis, our understanding of their functional dynamics during cellular senescence, particularly in cardiac fibrosis, remains limited. Senescent cells exhibit profound changes in endo‐lysosomal redox status and iron dynamics, yet tracking these alterations in real time within living cells poses significant challenges. Conventional biochemical assays often lack spatial resolution and temporal sensitivity, while fluorescent prob

Wiley2026

Membrane Protein Design: From Reprogramming Functions to AI-Guided De Novo Design Approaches

Computational membrane protein design has rapidly evolved from early physics-based modeling into a mature discipline powered by high-throughput experimental screening and cutting-edge AI technologies. The convergence of these approaches is transforming our capacity to probe natural membrane protein systems and to engineer sophisticated cell-based functions. Membrane proteins, in particular, offer a uniquely fertile landscape for computational engineering, enabling the stabilization of challenging therapeuti

American Chemical Society2026

IAP retrotransposons contribute to the transcriptional diversity of the murine placenta

Transposable elements (TEs) have made important contributions to the evolution of the placenta, and are argued to have played a role in the wide inter-species diversification of this critical developmental organ. Co-option of TEs by host genomes has led to the genesis of important placental genes, as well as trophoblast-specific gene regulatory elements. In mice, past work has demonstrated how multiple species-specific TE subfamilies are used as transcriptional enhancers in trophoblast stem cells. However,

openRxiv2026

Dissecting the impact of transcription factor dose on cell reprogramming heterogeneity using scTF-seq

Reprogramming often yields heterogeneous cell fates, yet the underlying mechanisms remain poorly understood. To address this, we developed single-cell transcription factor sequencing (scTF-seq), a single-cell technique that induces barcoded, doxycycline-inducible TF overexpression and quantifies TF dose-dependent transcriptomic changes. Applied to mouse embryonic multipotent stromal cells, scTF-seq generated a gain-of-function atlas for 384 mouse TFs, identifying key regulators of lineage specification, cel

Springer Science and Business Media LLC2025

A genome-wide, CRISPR-based screen reveals new requirements for translation initiation and ubiquitination in driving adipogenic fate change

In response to excess nutrients, white adipose tissue expands by both generating new adipocytes and upregulating lipogenesis in existing adipocytes. Here, we performed a genome-wide functional CRISPR screen to identify regulators of adipogenesis in the mouse 3T3-L1 preadipocyte model. In this pooled screening strategy, we used FACS to isolate populations based on lipid content, gating for fluorescence intensity of lipophilic fluorescent BODIPY dye. Additionally, we categorized whether the gene functions pri

Cold Spring Harbor Laboratory2025

DMG-35. Rewriting cellular fate: reprogramming diffuse midline glioma cells into post-mitotic cells

Background Diffuse midline gliomas (DMGs) are amongst the most lethal pediatric brain tumors. Patients diagnosed with DMG face a dismal prognosis with an overall survival of 9-12 months from diagnosis. Somatic H3 K27M mutations drive global hypomethylation and maintains the stemness phenotype of these tumors. The lack of terminal differentiation provides the opportunity for pharmacological reprogramming of DMGs to non-malignant cells as shown in other cancers including breast cancer and glioblastoma. Here,

Oxford University Press (OUP)2025

Epithelial Reprogramming by OM-89 Enhances Antibiotic Clearance of Uropathogenic E. coli in a Bladder Organoid Model

Recurrent urinary tract infections (UTIs) are a major clinical burden, often driven by the ability of uropathogenic Escherichia coli (UPEC) to persist intracellularly within bladder epithelial cells, protected from antibiotics and host immune responses. While vaccine and antibiotic alternatives are being explored, strategies that enhance epithelial cell-intrinsic defense mechanisms remain sparse. Here, we show that OM-89 (Uro-Vaxom®), an E. coli lysate, reprograms epithelial antimicrobial responses to enhan

Cold Spring Harbor Laboratory2025

Pharmacological induction of a progenitor state for the efficient expansion of primary human hepatocytes

Didier Trono, Matthias Lütolf, Evaristo José Planet Letschert, Marc Samuel Friedli, Nathalie Brandenberg, Marco Cassano, Carmen Maria Unzu Ezquerro

The liver is an organ with strong regenerative capacity, yet primary hepatocytes have a low amplification potential in vitro, a major limitation for the cell-based therapy of liver disorders and for ex vivo biological screens. Induced pluripotent stem cell ...

2019

Machine Learning Identifies Stemness Features Associated with Oncogenic Dedifferentiation

Jia Liu, Jian Wang, Jun Li, Min Wang

Cancer progression involves the gradual loss of a differentiated phenotype and acquisition of progenitor and stem-cell-like features. Here, we provide novel stemness indices for assessing the degree of oncogenic dedifferentiation. We used an innovative one ...

2018

Bimodal CD40/Fas-Dependent Crosstalk between iNKT Cells and Tumor-Associated Macrophages Impairs Prostate Cancer Progression

Michele De Palma

Heterotypic cellular and molecular interactions in the tumor microenvironment (TME) control cancer progression. Here, we show that CD1d-restricted invariant natural killer (iNKT) cells control prostate cancer (PCa) progression by sculpting the TME. In a mo ...

2018

Non-integrating episomal plasmid-based reprogramming of human amniotic fluid stem cells into induced pluripotent stem cells in chemically defined conditions

Amniotic fluid stem cells (AFSC) represent an attractive potential cell source for fetal and pediatric cell-based therapies. However, upgrading them to pluripotency confers refractoriness toward senescence, higher proliferation rate and unlimited different ...

Taylor & Francis Inc2016

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Related publications (11)

Nanodiamond‐Based Quantum Sensing Reveals Changes in Endo‐Lysosomal Activity of Stimulated Cardiac Fibroblasts in a Cellular Fibrosis Model

Wiley2026

A genome-wide, CRISPR-based screen reveals new requirements for translation initiation and ubiquitination in driving adipogenic fate change

Cold Spring Harbor Laboratory2025

Bimodal CD40/Fas-Dependent Crosstalk between iNKT Cells and Tumor-Associated Macrophages Impairs Prostate Cancer Progression

Michele De Palma

2018

Non-integrating episomal plasmid-based reprogramming of human amniotic fluid stem cells into induced pluripotent stem cells in chemically defined conditions

Taylor & Francis Inc2016