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Research methods

Related publications (29)

Associations between in vitro, in vivo and in silico cell classes in mouse primary visual cortex

Werner Alfons Hilda Van Geit

The brain consists of many cell classes yet in vivo electrophysiology recordings are typically unable to identify and monitor their activity in the behaving animal. Here, we employed a systematic approach to link cellular, multi-modal in vitro properties f ...

Nature Research2023

In vivo and ex vivo range of motion in the fire salamander Salamandra salamandra

Enrico Alexander Eberhard

Joint range of motion (RoM) analyses are fundamental to our understanding of how an animal moves throughout its ecosystem. Recent technological advances allow for more detailed quantification of this RoM (e.g. including interaction of degrees of freedom) b ...

2022

In vitro and in vivo NIR fluorescence lifetime imaging with a time-gated SPAD camera

Edoardo Charbon, Claudio Bruschini, Arin Can Ülkü

Near-infrared (NIR) fluorescence lifetime imaging (FLI) provides a unique contrast mechanism to monitor biological parameters and molecular events in vivo. Single-photon avalanche diode (SPAD) cameras have been recently demonstrated in FLI microscopy (FLIM ...

Optica Publishing Group2022

Novel trehalose-based excipients for stabilizing nebulized anti-SARS-CoV-2 antibody

Sandrine Gerber, Solène Marcelle Françoise Marie Passemard, François Rémi Pierre Noverraz

COVID-19 is caused by the infection of the lungs by SARS-CoV-2. Monoclonal antibodies, such as sotrovimab, showed great efficiency in neutralizing the virus before its internalization by lung epithelial cells. However, parenteral routes are still the prefe ...

2023

Determination of Aortic Characteristic Impedance and Total Arterial Compliance From Regional Pulse Wave Velocities Using Machine Learning: An in-silico Study

Nikolaos Stergiopoulos, Vasiliki Bikia, Georgios Rovas, Stamatia Zoi Pagoulatou

In-vivo assessment of aortic characteristic impedance (Zao) and total arterial compliance (CT) has been hampered by the need for either invasive or inconvenient and expensive methods to access simultaneous recordings of aortic pressure and flow, wall thick ...

2021

Insights gained from a comprehensive all-against-all transcription factor binding motif benchmarking study

Philipp Bucher, Giovanna Ambrosini, Romain Fernand Pietro Groux

BackgroundPositional weight matrix (PWM) is a de facto standard model to describe transcription factor (TF) DNA binding specificities. PWMs inferred from in vivo or in vitro data are stored in many databases and used in a plethora of biological application ...

2020

Extending the Concept of Predicting Fish Acute Toxicity In Vitro to the Intestinal Cell Line RTgutGC

Kristin Schirmer, Hannah Inka Schug

Testing chemicals for fish acute toxicity is a legal requirement in many countries as part of environmental risk assessment. To reduce the number of fish used, substantial efforts have been focused on alternative approaches. Prominently, the cell viability ...

2020

Innovative Tools and in vivo Methods for Tuberculosis Drug Discovery and Development

Raphael Christopher Sommer

Worldwide, tuberculosis (TB) is the leading cause of death due to a single infectious agent, claiming 1.6 million human lives and causing >10 million new cases in 2017 alone, mostly in low-income regions. The intracellular pathogen Mycobacterium tuberculos ...

EPFL2019

Rapid evaluation of bioactive Ti-based surfaces using an in vitro titration method

Paul Bowen, Jacques Lemaître, Weitian Zhao

The prediction of implant behavior in vivo by the use of easy-to-perform in vitro methods is of great interest in biomaterials research. Simulated body fluids (SBFs) have been proposed and widely used to evaluate the bone-bonding ability of implant materia ...

Springer2019

Synthesis, biology, computational studies and in vitro controlled release of new isoniazid-based adamantane derivatives

Stewart Cole, Anthony Vocat

Aim: There is a necessity for new drugs to be more efficient than today's standard due to the emergence of drug-resistant strains of Mycobacterium tuberculosis (Mtb) Results/methodology: 12 new isoniazid-based adamantane derivatives were synthesized and te ...

FUTURE SCI LTD2019

Pharmacokinetics and biodistribution study of self-assembled Gd-micelles demonstrating blood-pool contrast enhancement for MRI

Lothar Helm, Yohan Van de Looij

Magnetic resonance angiography (MRA) requires the use of contrast agents (CAs) to enable accurate diagnosis. There are currently no CAs on the market with appropriate pharmacokinetic (PK) parameters, namely long persistence in the blood, that can be easily ...

2019

Microfluidic and micromechanical in vivo studies of Caenorhabditis elegans

Roger Philippe Krenger

Caenorhabditis elegans is one of the most attractive model organisms in biomedical research for understanding human diseases and for drug testing at a whole-organism level, since many biological pathways and genes have been conserved between itself and hum ...

EPFL2019

An ecotoxicological view on neurotoxicity assessment

The numbers of potential neurotoxicants in the environment are raising and pose a great risk for humans and the environment. Currently neurotoxicity assessment is mostly performed to predict and prevent harm to human populations. Despite all the efforts in ...

SPRINGEROPEN2018

In vivo imaging with microsphere-based super-resolution microscopy

Martinus Adela Maria Gijs, Gergely Huszka, Roger Philippe Krenger

Employing dielectric microspheres in a standard microscope setup is an alternative to image sub-diffraction features optically, however this method suffers from a limited field-of-view. Here, we show our microsphere-based optical super-resolution microscop ...

IEEE2018

Calcium phosphate formation on titanium-based surfaces: bioactivity evaluated by in vitro methods

Weitian Zhao

The prediction of implant behaviors in vivo by the use of easy-to-perform in vitro methods is of great interest in biomaterials research. In the field of bone implants, it is commonly accepted that a spontaneous formation of a hydroxyapatite (HA) layer on ...

EPFL2018

Peptide-based coatings for flexible implantable neural interfaces

Silvestro Micera

In the last decade, the use of flexible biosensors for neuroprosthetic and translational applications has widely increased. Among them, the polyimide (PI)-based thin-film electrodes got a large popularity. However, the usability of these devices is still h ...

Nature Research2018

Subsurface ablation of tissue by ultrafast laser

Thomas Jean Victor Marie Lanvin

Laser-induced optical breakdown (LIOB) is a multiphoton process which can be used for selective removal of material. It revolves around the creation of a plasma in the focal volume of a beam, and requires very high peak intensities in the order of the GW.c ...

EPFL2017

Protein Corona: Impact of Lymph Versus Blood in a Complex In Vitro Environment

Heinrich Hofmann, Duncan Thomas Lindsay Alexander, Marc Moniatte, Marijana Mionic Ebersold, Débora Bonvin, Ulrich Aschauer, Diego Chiappe

In biological environments, the surface of nanoparticles (NPs) are modified by protein corona (PC) that determines their biological behavior. Unfortunately, in vitro tests still give different PC than in vivo tests causing in vitro-in vivo discrepancy; hen ...

Wiley-Blackwell2017

In-Vivo Validation of Fully Implantable Multi-Panel Devices for Remote Monitoring of Metabolism

Giovanni De Micheli, Sandro Carrara, Catherine Dehollain, Camilla Baj-Rossi, Francesca Stradolini, Andrea Cavallini, Enver Gürhan Kilinç

This paper presents the in-vivo tests on a Fully Implantable Multi-Panel Devices for Remote Monitoring of endogenous and exogenous analytes. To investigate issues on biocompatibility, three different covers have been designed, realized and tested in ...

Ieee-Inst Electrical Electronics Engineers Inc2016

Contribution of polymeric materials to progress in xenotransplantation of microencapsulated cells - A review

Sandrine Gerber, Jutta Christine Wandrey, Redouan Mahou, Solène Marcelle Françoise Marie Passemard, François Rémi Pierre Noverraz

Cell microencapsulation and subsequent transplantation of the microencapsulated cells require multidisciplinary approaches. Physical, chemical, biological, engineering, and medical expertise has to be combined. Several natural and synthetic polymeric mater ...

2016